Bacterial Detection Causing Subclinical Mastitis on Etawah Crossbreed Goat in Kokap, Kulon Progo, Yogyakarta Province

Resident in Kokap, Kulon Progo, Yogyakarta Province has another income sector, that is dairy goat (Etawah Crossbreed) farming and they have been joining into farm group, which is called Kelompok Ternak. There are many bacteria that causing dairy goat intra mamary infection, thus the milk production will be contaminated. Two hundred and four (204) milk samples from 103 lactation goat in four farm groups in Kokap, Kulon Progo, Yogyakarta is used in this study. Out of these, 21 samples (10,29%) were detected strong positive (++ and +++) for subclinical mastitis by using California Mastitis Test (CMT). Among this positive result, the next step was bacteria detection. This tests were held in Preclinic Laboratory Animal Health Study Program, Vocational School, UGM. The samples were inoculated in blood agar, then colony was identify by Gram staining. The next steph is biochemical tests using McConkey Agar (MCA) and eosin methylene blue (EMB) as a selective media for Gram negative bacteria,  manitol salt agar (MSA) as selective media for Staphylococcus, sugar fermented broth (glucose, lactose, mannitol, maltose, and sacharose), Kigler iron agar (KIA), lysine iron agar (LIA), motility indole ornithin (MIO), and Simmon’s citrate. The result was Staphylococcus aureus (80%), Staphylococcus epidermidis (20%), Escherichia coli (10%), and Klebsiella pneumoniae (10%).

Deteksi Bakteri Penyebab Mastitis Subklinis pada Kambing Peranakan Etawah di Kokap, Kulon Progo, Daerah Istimewa Yogyakarta

Resident in Kokap, Kulon Progo, Yogyakarta Province has another income sector, that is dairy goat (Etawah Crossbreed) farming and they have been joining into farm group, which is called Kelompok Ternak. There are many bacteria that causing dairy goat intra mamary infection, thus the milk production will be contaminated. Two hundred and four (204) milk samples from 103 lactation goat in four farm groups in Kokap, Kulon Progo, Yogyakarta is used in this study. Out of these, 21 samples (10,29%) were detected strong positive (++ and +++) for subclinical mastitis by using California Mastitis Test (CMT). Among this positive result, the next step was bacteria detection. This tests were held in Preclinic Laboratory Animal Health Study Program, Vocational School, UGM. The samples were inoculated in blood agar, then colony was identify by Gram staining. The next steph is biochemical tests using McConkey Agar (MCA) and eosin methylene blue (EMB) as a selective media for Gram negative bacteria, manitol salt agar (MSA) as selective media for Staphylococcus, sugar fermented broth (glucose, lactose, mannitol, maltose, and sacharose), Kigler iron agar (KIA), lysine iron agar (LIA), motility indole ornithin (MIO), and Simmon’s citrate. The result was Staphylococcus aureus (80%), Staphylococcus epidermidis (20%), Escherichia coli (10%), and Klebsiella pneumoniae (10%).

Karakterisasi Feno-Genotipik Kemampuan Hemolisa Isolat Staphylococcus aureus Asal Susu Kambing Mastitis dan Daging Ayam Segar

Abstract  Staphylococcus aureus (S. aureus) is a pathogenic bacterium with many virulence factors, such as toxins, immunomodulatory factors, and exoenzymes. Hemolysin is one of the essential toxins in the pathogenesis of S. aureus, with the action of lysing erythrocytes and other cells. This study aimed to determine the phenotypic and genotypic characteristics of S. aureus isolates from animal origin. Eight S. aureus isolates from goat mastitis and raw chicken meat phenotypically evaluated the ability to hemolyze sheep blood. At the same time, genotypic were determinated by detecting the hla and hlb genes. The results showed that most 87.5% (7/8) of S. aureus isolates had β-hemolysis phenotypic. PCR determination showed that all (8/8) isolates were positive for the α-toxin (hla) encoding gene, while half (4/8) of the isolates were positive for the β-toxin (hlb) encoding gene. The use of 5% defibrinated sheep blood agar plates is recommended to determine the hemolytic character of S. aureus tested. Keywords: Character; Hemolysis; S. aureus   Abstrak  Staphylococcus aureus (S. aureus) merupakan bakteri patogen yang memiliki banyak faktor virulensi seperti toksin, faktor imunomodulator, dan eksoenzim. Hemolisin merupakan salah satu toksin penting dalam patogenesis S. aureus, dengan aksi kerja melisiskan eritrosit dan sel lainnya. Penelitian ini bertujuan untuk mengetahui karakter hemolisa isolat S. aureus asal hewan secara fenotipik dan genotipik. Delapan isolat S. aureus asal susu kambing mastitis dan daging ayam segar dievaluasi secara fenotipik untuk melihat kemampuan menghemolisa darah domba, sedangkan determinasi genotipik dengan deteksi gen hla dan gen hlb. Hasil penelitian menunjukkan mayoritas 87.5% (7/8) isolat S. aureus memiliki fenotipik β-hemolisa. Determinasi PCR menunjukkan 100% (8/8) isolat positif gen penyandi α-toksin (hla), sedangkan 50% (4/8) isolat positif gen penyandi β-toksin (hlb). Penggunaan 5% defibrinated pelat agar darah domba lebih direkomendasikan untuk melihat karakter hemolisa S. aureus yang diuji. Kata kunci: Hemolisa; Karakter; S. aureu