Functional Properties of Gelatin Hydrolysate from Salmon Skin (Salmo salar)

Gelatin hydrolysate is derived from a hydrolysis of gelatin (denatured collagen) to expose the functional properties of the hydrolysate obtained. This study was carried out to determine functional properties of gelatin hydrolysate from salmon skin as influenced by the degree of enzymatic hydrolysis. Aqueous extraction of gelatin from salmon skin was done at 45oC for 60 minutes. The extracted gelatin was then hydrolysed using 1% alcalase at 54.5oC and pH 8. The functional properties determined were molecular weight, solubility, foaming capacity and stability, emulsifying activity and stability index were analysed. It was found that different time of hydrolysis (5, 15, 45 and 180 minutes) resulted in different degree of hydrolysis (DH) (10, 20, 30 and 40%, respectively). The gelatin hydrolysate at 40% DH showed the highest solubility (p<0.05). However, the foaming and emulsifying properties were the highest at 10% degree of hydrolysis (p<0.05)

Kesan masa pendidihan dan simulasi pencernaan ke atas protein dan hidrolisat protein yang dihasilkan daripada sarang burung walit spesies Aerodramus fuciphagus

Sarang burung walit (SBW) dengan kandungan manfaat kesihatan yang pelbagai dihasilkan daripada burung walit Aerodramus fuciphagus. Sarang tersebut dihasilkan menggunakan air liur burung walit yang mengandungi bahan glikoprotein yang tinggi. Kajian ini dijalankan untuk menghasilkan hidrolisat protein SBW dan seterusnya menentukan kesan masa pendidihan (0 - 180 minit) dan proses pencernaan ke atas sampel SBW dan hidrolisat protein SBW. Proses hidrolisis berenzim telah dijalankan dengan menggunakan enzim alkalase untuk menghasilkan hidrolisat protein SBW. Ujian pencernaan terhadap protein SBW dan hidrolisat yang dihasilkan telah dijalankan menggunakan simulasi sistem pencernaan manusia in vitro. Hasil kajian menunjukkan dengan peningkatan masa pendidihan sehingga 180 minit, kandungan peptida yang terhasil daripada pendidihan SBW mentah dan hidrolisat adalah berbeza secara signifikan (p<0.05) dengan nilai kandungan peptida daripada SBW mentah terdidih didapati lebih tinggi daripada hidrolisat SBW. Seterusnya, hasil daripada pencernaan protein terhadap SBW dan hidrolisat SBW menunjukkan nilai kandungan peptida yang berbeza secara signifikan (p<0.05) dengan darjah hidrolisis protein SBW didapati paling tinggi (96.55%); diikuti dengan hidrolisat SBW 60 minit (88.69%), 120 minit (89.32%) dan 180 minit (89.81%) yang tidak berbeza secara signifikan (p<0.05) antara sampel. Hasil kajian ini menunjukkan SBW didegradasi secara aktif dalam masa 30 minit pendidihan berbanding hidrolisat SBW. Perbezaan tersebut menjelaskan bahawa komponen protein pada hidrolisat SBW telah banyak dicernakan oleh tindak balas hidrolisis berenzim dalam penyediaan hidrolisat tersebut. Proses pencernaan in vitro pula menunjukkan bahawa protein SBW dan hidrolisatnya adalah protein makanan yang boleh dicernakan dengan baik oleh sistem pencernaan manusia

Edible bird’s nest: physicochemical properties, production, and application of bioactive extracts and glycopeptides

Edible bird’s nest (EBN), the swiftlet’s nest, has been consumed for centuries as a tonic or health food. The information upon the usage and benefits of the EBN are largely based on historical and observational reports since its discovery, until some were proven scientifically at recent times. Studies have been conducted on the major components of the EBN, i.e. glycoprotein, in attempts to elucidate the roles of the glycoprotein in terms of functionality toward health improvement. This article presents a review of the physicochemical and nutritional composition of EBN from different harvested locations and its functional properties. The conversion of EBN glycoproteins to glycopeptides via suitable hydrolysis methods to produce bioactive EBN glycopeptides was demonstrated, and it showed improvements in the functionality and nutritional values of EBN. Some applications of EBN glycopeptides into the new development of food and beverage products showed positive results, implicated to the bioavailability of the EBN glycopeptides. The combined information in this review will benefit the scientific communities, EBN manufacturers, and nutraceutical industries

Kesan pencernaan gastrousus terhadap ciri fizikokimia dan kebiotersediaan antioksidan produk chia

Chia (Salvia hispanica L.) adalah bijirin pseudo yang kaya dengan asid lemak tak tepu (PUFA) dan protein berfungsi. Kajian ini bertujuan untuk menentukan sifat fizikokimia dan kebiotersediaan antioksidan produk chia iaitu tepung chia ternyah lemak (TCT), pencilan protein chia (IPC), hidrolisat protein chia (HPC) dan nanokapsul hidrolisat protein chia (nHPC). Simulasi model pencernaan gastrousus secara in-vitro telah mengasingkan protein chia kepada empat pecahan berbeza iaitu sampel sebelum dicerna (ND), fraksi tercerna pasca-gastrik (PG), fraksi tercerna pasca-usus yang diserap oleh kolon (PUa) dan fraksi tercerna pasca-usus yang tertinggal dalam kolon (PUb). Sampel nHPC mencatatkan darjah hidrolisis (DH) paling rendah (p<0.05) (19.72%) selepas fasa pencernaan gastrik dan tiada perubahan signifikan (p<0.05) selepas pencernaan usus. Penyusutan (p<0.05) nilai asid amino hidrofobik (AAH) dan asid amino aromatik (AAR) bagi sampel nHPC direkodkan selepas pencernaan gastrousus (PUa), masing-masing sebanyak 4.81 dan 3.95%. Berbanding semua sampel, HPC dan nHPC mencatatkan nilai tertinggi (p<0.05) potensi pemerangkapan radikal DPPH dan ABTS sampel TCT dan IPC, sebaliknya mengurangkan (p<0.05) potensi antioksidan bagi sampel HPC. Berdasarkan ujian FRAP, pencernaan gastrousus tidak memberi kesan (p<0.05) terhadap kapasiti antioksidan bagi sampel nHPC. Kesimpulannya, pencernaan gastrousus mempengaruhi sifat fisikokimia dan kebiotersediaan antioksidan produk chia yang dikaji, memberikan kefahaman penting tentang manfaat kesihatan dan aplikasi produk chia dalam diet pemakanan manusia

Effect of Molecular Weight Reduction of Polypeptides on Angiotensin Converting Enzyme (ACE) Inhibitory Activity in Chicken Skin Hydrolysate (Collagen)

Inhibition of Angiotensin Converting Enzyme (ACE) reduces blood pressure and gives an anti-hypertensive effect. Chicken skin is an undesirable by-product of the poultry industry, disliked by consumer because of the high fat content. The aim of this research is to determine the effect of molecular weight reduction on ACE inhibition activity in chicken skin hydrolysate. Chicken skin is prepared by manually defatting, soaked in acetone and in 0.1M phosphate buffer. Sample hydrolysis is carried out using alcalase enzyme for a duration of 4 hours at 60°C and pH 9.5. The best degree of hydrolysis (DH), at 4th hour, with value of 49.54 ± 0.79 %, is ultrafiltrated and used in ACE inhibition activity detection. The sample weight ≥ 10 kDa , 3 - 10 kDa and ≤ 3 kDa contains 5.63 ± 0.01 g/L, 2.84 ± 0.06 g/L and 1.07 ± 0.18 g/L peptide content respectively whereas soluble protein content is 0.51 mg/mL for sample weight ≥ 10 kDa, 0.27 mg/mL for sample weight 3 - 10 kDa and 0.23 mg/mL for sample weight ≤ 3 kDa. The ACE inhibition activity in sample weight ≤ 3 kDa is highest with value of 80.38 ± 2.69% followed by sample weight 3 - 10 kDa with a value of 49.40 ± 2.63% and sample weight ≥ 10 kDa with value of 42.73 ± 5.08%. Significant differences ( P ≤ 0.05) exist between sample weight ≤ 3 kDa and &gt; 3 kDa. This research shows that molecular weight reduction increases ACE inhibition activity

Bioactive sialylated-mucin (SiaMuc) glycopeptide produced from enzymatic hydrolysis of edible swiftlet’s nest (ESN): degree of hydrolysis, nutritional bioavailability, and physicochemical characteristics

The swiftlet’s nest is an edible bird’s nest (EBN) produced from dried gelatinized saliva secreted by swiftlets during the breeding season. It is widely used in industries for its high nutritional values and health benefits. However, the utilization of ESN is restricted due to its physicochemical properties, such as insolubility. The recently invented bioactive ESN hydrolyzate is reported to improve the ESN’s solubility, functional and nutritional properties to broaden its application. This study has produced bioactive ESN hydrolyzates with different hydrolysis periods to investigate the optimum conditions to achieve maximized degree of hydrolysis (DH) and to compare the physicochemical differences. The result revealed that optimum ESN hydrolysis was at 90 minutes, in which the DH and enhanced solubility (three folds) indicated a complete breakdown of glycoprotein into bioactive glycopeptide. This result is in line with the physicochemical analyses result. The hydrolysis has significantly decreased (p ≤ .05) the ESN protein content, while the levels for peptide, glycopeptide, polysaccharides, and sialic acid showed the opposite. Interestingly, the amino acid from raw ESN and hydrolyzates in all incubation periods showed no significant difference (p ≤ .05). This result suggests that the ESN biological properties remained unchanged with alcalase hydrolysis. Therefore, the findings in this study confirm the feasibility of bioactive ESN hydrolyzate as a versatile product suitable for various industries in utilizing ESN in whole instead of as extracts