Uji Efikasi Bioinsektisida Jamur Entomopatogen Berformulasi Cair Terhadap Plutella Xylostella (L.) Di Laboratorium

Efficacy test of liquid bio-insecticide of entomopathogenic fungi in control against Plutella xylostella in the laboratory. The insect pest P. xylostella could reduce crop production of Brassicaceae. The aim of research was to test the efficacy liquid bio insecticide with active ingredient of Beauveria bassiana and Metarhizium anisopliae fungi to control P. xylostella. Bio-insecticide was applied by spraying on mustard leaves infested with 50 individuals of third instar larvae of P. xylostella and a density of 1x106 conidia ml-1. Larval mortality was observed every 2 hours and LT50 of larvae was calculated. The study showed that the highest percentage of mortality found in Mt ES and Mt ES (cf) isolates was 99.6%, the lowest mortality at Mt NES isolate was 96.80%. LT50 and LT95 values Bb ES were the lowest i.e. 2.04 days and 2.95 days. The highest LT50 and LT95 of Mt NES isolate were 2.24 days and 3.32 days. The liquid bio-insecticide of entomopathogenic fungus B. bassiana and M. anisopliae were effective to control the larvae of P. xylostella

EKSPLORASI, ISOLASI DAN SELEKSI JAMUR ENTOMOPATOGEN PLUTELLA XYLOSTELLA (LEPIDOPTERA: YPONOMEUTIDAE) PADA PERTANAMAN CAISIN (BRASSICA CHINENSIS) DI SUMATERA SELATAN

Plutella xylostella is the most destructive insect pests of the brassicae family. The research objective was to explore, isolate and select entomopathogenic fungi as biological agents for control of P. xylostella. This study used 20 fungal isolates originating from soil and infected insects around the farmers' field in lowland and highland of South Sumatra. The fungal isolates were tested to third instar larvae of P.xylostella. The suspension of entomopathogenic fungus was topical inoculated with a density of 1x106 conidia ml-1 on the test insect and five replicates. The result showed that the highest (83%) and the lowest (41%) mortality of the larvae P.xylostella was induced by fungal BPluS and BNIPTr, respectively. Moreover, the shortest (2.1 days) and the highest (4.3days) lethal times of the infected host were induced by fungal BPluS and BNIPTr, respectively