Peripheral Nervous System Genes Expressed in Central Neurons Induce Growth on Inhibitory Substrates

Trauma to the spinal cord and brain can result in irreparable loss of function. This failure of recovery is in part due to inhibition of axon regeneration by myelin and chondroitin sulfate proteoglycans (CSPGs). Peripheral nervous system (PNS) neurons exhibit increased regenerative ability compared to central nervous system neurons, even in the presence of inhibitory environments. Previously, we identified over a thousand genes differentially expressed in PNS neurons relative to CNS neurons. These genes represent intrinsic differences that may account for the PNS’s enhanced regenerative ability. Cerebellar neurons were transfected with cDNAs for each of these PNS genes to assess their ability to enhance neurite growth on inhibitory (CSPG) or permissive (laminin) substrates. Using high content analysis, we evaluated the phenotypic profile of each neuron to extract meaningful data for over 1100 genes. Several known growth associated proteins potentiated neurite growth on laminin. Most interestingly, novel genes were identified that promoted neurite growth on CSPGs (GPX3, EIF2B5, RBMX). Bioinformatic approaches also uncovered a number of novel gene families that altered neurite growth of CNS neurons

Product Lifecycle Management Strategy for the Definition and Design Process of Face Implants Oriented to Specific Patients

Part 2: Collaborative Environments and New Product DevelopmentInternational audienceThe main purpose of this research was oriented to generate a structured model from an organizational vision to the definition and development of precise osteosynthesis prosthesis. Implants were adapted to the Colombian population anthropometry allowing fracture reductions and craniofacial defects corrections based on technologies for specific patients. This research was developed taking into account the first three PLM stages: Imagination, definition, and realization. Procedures, stages, roles, and activities that take part in the design and pre-surgical planning were identified for the patient-specific implants PSI, carried out through a study case. It was established as a definition model for design and fabrication process of patient-specific implants (PSI). It was possible that technology included in a collaborative workflow wherein the roles which intervene in the design process and the pre-surgical planning were related. The ability to design implants for specific patients and surgical guides was obtained different pathology situations including face trauma. According to the PLM strategy for designing custom implant, it would be possible to build innovation capabilities. With those, an organization could generate a collaborative workflow integrating stages, roles, activities, applying technology and local human resource. Further work related to the subject is necessary to enhance the process by iteration and improve the clinical cases management