Molecular and phylogenetic investigation of Chiromantes boulengeri (Decapoda, Brachyura, Sesarmidae) species from Arvand river

In this study, the molecular characteristics of Chiromantes boulengeri were investigated from intertidal waters of Arvand River, Minoo Island in 2014. For this reason, samples from intertidal zones of two areas across Minoo Island were collected and preserved in 70% ethanol, followed by transferring to the laboratory for further examinations. Genomic DNA was extracted using phenol- chloroform method and mitochondrial gene 16SrRNA was sequenced and compared with other species from GenBank NCBI. The morphological examinations using identification keys and phylogeny sequence results showed that all specimens belonged to Chiromantes boulengeri in the study area. Phylogenetic analysis revealed that there are mutations within C. boulengeri from two areas of Arvand River. It seems that, this species most probably is adapted to the different ecological conditions in this area