Software for full-color 3D reconstruction of the biological tissues internal structure

A software for processing sets of full-color images of biological tissue histological sections is developed. We used histological sections obtained by the method of high-precision layer-by-layer grinding of frozen biological tissues. The software allows restoring the image of the tissue for an arbitrary cross-section of the tissue sample. Thus, our method is designed to create a full-color 3D reconstruction of the biological tissue structure. The resolution of 3D reconstruction is determined by the quality of the initial histological sections. The newly developed technology available to us provides a resolution of up to 5 - 10 {\mu}m in three dimensions.Comment: 11 pages, 8 figure

AUTOLOGOUS PLATELET-RICH PLASMA IN THE TREATMENT OF PATIENTS WITH KNEE ARTHRITIS III STAGE

The purpose of the study - to determine the effectiveness of using PRP in the treatment of patients with stage III knee arthritis. Material for the study - 81 patients , two clinical groups with unilateral ( 9 patients - 15%) or bilateral ( 51 patients - 85%) with stage III knee arthritis. The first group of patients in an amount of 40 people were treated by introducing into the knee joint of autologous platelet-rich plasma 1 every three weeks. In the comparison group of 41 patients were symptomatic treatment with non-steroidal anti-inflammatory drugs. Evaluation of the results was performed using the LEKEN scale, VAS, verbal evaluation of the effectiveness of pain at 3, 6, 9 weeks after the treatment. Results: satisfactory result has increased to 52.5 %±0.2% of patients in week 9 of the main group. Score VAS and LEKEN scale gives a clear picture to the marked reduction in pain 9 weeks to 4 observation points. Intraarticular injection of PRP in the treatment of patients with stage III can reduce the severity of pain, improve knee function and quality of life for 9 weeks during their preparation for surgical treatment. Adherence to treatment of patients with stage III receiving intraarticular PRP, close to 98%

3D bioactive coatings with a new type of porous ridge/cavity structure

In this study, biocompatibility of plasma-sprayed 3D CP Ti-coated wire implants has been evaluated. Plasma- sprayed 3D CP Ti coatings have a porous structure consisting of ridges and cavities whose height is equal to that of the thickness of the coating. The porosity of plasma-sprayed 3D CP Ti coatings was found to be higher in cavities and it made up 46%. To study the clinical success of these implants, post-mortem computed tomography (CT) of distal femurs of animals (mainly dogs) was carried out. In vivo studies failed to demonstrate an increase in the amount of new bone tissue around uncoated implants. The amount of new bone tissue around implants with additional ceramic coatings made up 60% after 8 weeks. The amount of new bone tissue around 3D CP Ti-coated implants was only 5% after 8 weeks; however, after 16 weeks, an intensive growth of bone tissue around implants was observed. The amount of new bone tissue around implants with an additional ceramic coating was insufficient after 16 weeks. Forty-eight weeks after implant placement, the distribution of bone tissue in 3D CP Ti, 3D CP Ti-HA- and 3D CP Ti-HA-CaP-coated implants was 74.5%, 89.2% and 92.5%, respectively. The amount of bone tissue around uncoated implants did not exceed 10%. 3D CP Ti-HA coatings demonstrated better osseointegration compared with 3D CP Ti coatings and were found to have higher shear strength (9.8 ± 1.54 MPa) at early stages of osseointegration