29 research outputs found
Agronomic behavior of banana cultivars in the geographic microregion of Assis, São Paulo, Brazil
Abstract The characterization of banana cultivars is an important stage, which allows the identification of materials adapted to a particular region. The aim of this study was to evaluate the agronomic performance in the edaphoclimatic conditions of the geographic microregion of Assis, State of São Paulo, during the first three production cycles. The experiment was installed at São José Farm, Palmital, SP, where ten banana cultivars (Grande Naine, IAC 2001, FHIA 02, Bucaneiro, FHIA 17, Calypso, Ambrosia, Thap Maeo, BRS Princesa and Caipira) were evaluated during the three production cycles. The experimental design was in randomized blocks, with four replicates, each plot with 8.75 m2 and 5 plants per plot. The evaluated variables were plant height, pseudostem perimeter, number of leaves at flowering and harvesting, bunch mass, number of hands per bunch, fruit and pulp yield. Results were submitted to analysis of variance, means comparison tests and multivariate clustering or tree clustering analysis. ‘Grande Naine’ and ‘IAC 2001’ cultivars, as well as those of the Cavendish subgroup, are the most suitable for cultivation in the geographic microregion of Assis, SP, considering production performance in the evaluated production cycles
Análise Do Fluxo Sanguíneo Nas Doenças Da Retina E Da Coroide, Utilizando Tecnologias Em Tomografia De Coerência Óptica
Purpose: To Analyze Blood Flow Of Chorioretinal Diseases Utilizing New Algorithms On Optical Coherence Tomography (Oct). Methods: Healthy Individuals And Patients With Chorioretinal Disorders Were Evaluated In Two Oct Devices: Spectral-Domain (Sd-Oct) And An Ultra-High Speed (400 Khz A-Scans/S) Swept-Source (Ss-Oct) Prototype. Comparison Between These Two Technologies Was Made, And New Algorithms Applied: En Face Doppler Oct, To Measure Total Retinal Blood Flow (Trbt) And; Variable Interscan Time Analysis (Vista), Pairing 1.5ms And 3ms Interscan Times To Create A Color-Coded Blood Flow Speed Display, Using Oct Angiography (Octa). Results: Total Choroidal Neovascularization (Cnv) Area Was Significantly Greater When Evaluated On Ss-Oct Compared To The Sd-Oct On Both 3"3mm, And 6"6mm Scan Size. En Face Doppler Oct Exhibited Lower Trbf In Eyes With Diabetic Macular Edema. In Healthy Subjects, Vista Showed Higher Speeds Flow Gradient Associated With The Larger Retinal Vasculatures And Slower Speeds Associated WithObjetivo: Realizar Análise Do Fluxo Sanguíneo Em Doenças Da Retina E Da Coroide, Utilizando Tecnologias Em Tomografia De Coerência Óptica (Oct). Métodos: Pessoas Saudáveis E Pacientes Acometidos Por Doenças Da Retina E Da Coroide Foram Analisados Utilizando Duas Tecnologias De Oct: Domínio-Espectral (Sd-Oct) E Protótipo Ultrarrápido (400 Khz A-Scans/S) De Fonte De Varredura (Ss-Oct). Foram Realizadas Comparações Entre As Duas Tecnologias E Aplicação De Novos Algoritmos: En Face Doppler Oct, Para Quantificar Fluxo Sanguíneo Total Da Retina (Fstr) E "Análise Variável Do Tempo Entre Scans" (Vista), Pareando Scans De 1,5 Ms E 3 Ms Para Gerar Um Mapa De Cores Da Velocidade Do Fluxo, Utilizando A Angiografia Por Oct (Octa). Resultados: A Área Total Da Neovascularização De Coroide (Nvc) Foi Significativamente Maior No Ss-Oct Do Que No Sd-Oct Tanto Nos Tamanhos 3"3mm, Quanto No 6"6mm. O Fstr Foi Significativamente Mais Baixo Em Pacientes Portadores De Edema Macular Diabético. A Análise Com O Algoritmo Vista Mostrou QDados abertos - Sucupira - Teses e dissertações (2018
Vital Dyes in Ophthalmology: a Chemical Perspective
Vital dyes have advanced diagnosis and surgical technique in various specialties, including oncology, gastroenterology and ophthalmology. Intra-operative and diagnostic dyes are finding uses in all areas of ophthalmology, including cornea, cataract, retina, glaucoma, orbit and conjunctiva. We provide a summary of current knowledge of the chemical concepts of vital dyes in ophthalmology. We review the properties of dyes, techniques of application, indications and complications in ocular surgery. Vital dyes represent an expanding area of research, and novel dyes deserve further investigation.Universidade Federal de São Paulo, Vis Inst, Paulista Sch Med, Dept Ophthalmol, São Paulo, BrazilUniversidade Federal de São Paulo, Vis Inst, Paulista Sch Med, Dept Ophthalmol, São Paulo, BrazilWeb of Scienc
Comparison of anterior segment optical coherence tomography angiography and fluorescein angiography for iris vasculature analysis
The aim of this study is to perform imaging of irises of different colors using spectral domain anterior segment optical coherence tomography angiography (AS-OCTA) and iris fluorescein angiography (IFA) and compare their effectiveness in examining iris vasculature. This is a cross-sectional observational clinical study. Patients with no vascular iris alterations and different pigmentation levels were recruited. Participants were imaged using OCTA adapted with an anterior segment lens and IFA with a confocal scanning laser ophthalmoscope (cSLO) adapted with an anterior segment lens. AS-OCTA and IFA images were then compared. Two blinded readers classified iris pigmentation and compared the percentage of visible vessels between OCTA and IFA images. Twenty eyes of 10 patients with different degrees of iris pigmentation were imaged using AS-OCTA and IFA. Significantly more visible iris vessels were observed using OCTA than using FA (W = 5.22p < 0.001). Iris pigmentation was negatively correlated to the percentage of visible vessels in both imaging methods (OCTA, rho = - 0.73, p < 0.001IFA, rho = - 0.77, p < 0.001). Unlike FA, AS-OCTA could not detect leakage of dye, delay, or impregnation. Nystagmus and inadequate fixation along with motion artifacts resulted in lower quality images in AS-OCTA than in IFA. AS-OCTA is a new imaging modality which allows analysis of iris vasculature. In both AS-OCTA and IFA, iris pigmentation caused vasculature imaging blockage, but AS-OCTA provided more detailed iris vasculature images than IFA. Additional studies including different iris pathologies are needed to determine the most optimal scanning parameters in OCTA of the anterior segment.Univ Fed Sao Paulo, Dept Oftalmol & Ciencias Visuais, Escola Paulista Med, Sao Paulo, BrazilPontificia Univ Catolica Valparaiso, Fac Ciencias, Carrera Tecnol Med, Valparaiso, ChileCAPES CNPq, PEC PG, Programa Estudantes Convenio Posgrad, Sao Paulo, BrazilUniv Illinois, Dept Ophthalmol & Visual Sci, Chicago, IL USAUniv Fed Sao Paulo, Dept Oftalmol & Ciencias Visuais, Escola Paulista Med, Sao Paulo, BrazilWeb of Scienc
Large colloid drusen analyzed with structural en face optical coherence tomography
Drusen are extracellular deposits between the basal lamina of the retinal pigment epithelium (RPE) and the inner collagenous layer of Bruch's membrane. Large colloid drusen (LCD) are located below the RPE and are characterized by multiple, large, dome-shaped RPE detachments, with marked attenuation of the ellipsoid zone overlaying the drusen. This report presents the structural en face optical coherence tomography (OCT) findings of LCD and relates them to findings from fluorescein and indocyanine green angiography. We describe the case of a 55-year-old woman who presented with the chief complaint of a 5-year history of progressively worsening vision. Her best-corrected visual acuities were 20/40 and 20/400 in the right eye and the left eye, respectively. Fundus examination showed large bilateral, symmetrical, sub-retinal, yellowish lesions compatible with LCD. We describe the structural en face OCT characteristics and angiographic findings from this patient.Hosp Brigadeiro, Dept Ophthalmol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo UNIFESP, Dept Ophthalmol & Visual Sci, EPM, Sao Paulo, SP, BrazilUniv Fed Sao Paulo UNIFESP, Dept Ophthalmol & Visual Sci, EPM, Sao Paulo, SP, BrazilWeb of Scienc
Development of an Experimental Model of Proliferative Retinopathy by Intravitreal Injection of VEGF165
Purpose: To develop an experimental model of proliferative retinopathy by intravitreal injection of vascular endothelial growth factor 165 (VEGF165) in pigmented rabbits.Methods: A prospective, controlled, comparative intervention study. Six pigmented rabbits (Chinchilla breed) were subjected to intravitreal injection of VEGF165 in their right eye. the left eye was used as control and received an injection of balanced salt solution. in group 1, 3 rabbits received a 10-mu g injection, and in group 2, 3 rabbits received a 20-mu g injection. At baseline, all subjects were analyzed by anterior biomicroscopy, retinography, fluorescein angiography, and optical coherence tomography (OCT) fundus images. Biomicroscopy and all ancillary examinations were repeated at weeks 1, 2, and 5. in the fifth week after the injection, the rabbits were euthanized and the eyes were enucleated and subjected to histological evaluation.Results: Seven days after the intravitreal VEGF165 injection, all rabbits developed intense neovascularization of the retina and anterior segment. Neovascularization of the posterior pole was similar in both groups, and the anterior segment was more florid in group 2. At weeks 1 and 2, neovascularization persisted with a minor decrease in conjunctival hyperemia in both groups. At week 5, there was a partial regression of neovascularization of the posterior pole, which was more prominent in group 1 than group 2, with persistent anterior neovascularization in both groups. OCT showed a statistically significant increase in retinal thickness, hyaloid detachment, and tractional retinal detachment. After the 5-week period, ocular histopathological evaluation showed an increase in retinal thickness, hyaloid detachment, and intense neovascularization in both groups, especially group 2.Conclusion: This pilot study of a neovascularization model using intravitreal injection of VEGF165 in pigmented rabbits showed that both doses of 10 and 20 mu g were successful and effective in inducing vascular growth in the retina and anterior segment and can therefore be used for evaluating drug efficacy in future studies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Dept Ophthalmol, Vis Inst IPEPO, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ophthalmol, Vis Inst IPEPO, BR-04023062 São Paulo, BrazilFAPESP: 2011/17363-3Web of Scienc
Optical coherence tomography angiography features in retinal artery occlusion
Univ Fed Sao Paulo, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Sao Paulo, SP, BrazilWeb of Scienc