2 research outputs found
Melatonin as a new postharvest treatment for increasing cut carnation (Dianthus caryophyllus L.) vase life
The marketability of cut flowers is directly affected by their vase life, which determines acceptability for commercial
purposes. In carnations and other species of cut flowers, corolla is one of the most affected parts during
flower senescence due to the petal withering which is accelerated by metabolic processes occurring after separation
from the mother plant. Melatonin (MT) is a compound with antioxidant properties, naturally present in
plant tissues that plays important roles in the regulation of different metabolic processes. In this research work
the effect of different MT concentrations (0.01, 0.1 and 1 mM) on the vase life of cut carnations flowers cv.
Baltico was evaluated. The greatest delay in senescence was observed with 0.1 mM MT concentration, increasing
vase life up to 10 days more as compared to control carnations. Although all MT concentrations assayed
significantly (P < 0.05) maintained initial levels of fresh weight, membrane stability index, bioactive compounds
and antioxidant activity for longer time, the lowest concentrations were those that had the most relevant impact
on vase life. The highest dose evaluated (1 mM) maintained all the parameters evaluated but showed the wilting
symptoms earlier. For this reason, 0.1 MT concentration could be a tool capable of improving carnation vase life
for longer time, increasing the commercial potential of this cut flower
Extraction Processes with Several Solvents on Total Bioactive Compounds in Different Organs of Three Medicinal Plants
The extraction of secondary metabolites by water, MeOH:water (8:2) containing NaF, methanol, ethanol and acetone (all of them diluted (7:3) in water)from the different parts (leaves, flowers, stems and roots) of Passiflora caerulea L., Physalis peruviana L. and Solanum muricatum Aiton via decoction and maceration methods was studied. The highest extraction yields were recorded by methanol for decoction and acetone for maceration. The total polyphenol content (TPC) obtained by decoction had the highest TPC contents, and MeOH containing NaF was the best solvent for the extraction of TPC. Maceration was suitable for flavonoid extractions, with ethanol and acetone being the best solvents. In general, the highest levels of TPC and flavonoids were obtained from Passiflora leaves regardless of the solvent or extraction method applied. Furthermore, the roots of Physalis and Solanum showed important levels of these compounds in consonance with the total antioxidant activity (TAA) evaluated in the different organs of the plant in the three species. In this study, the solvents and extraction methods applied were tools that determined significantly the level of extraction of bioactive compounds, showing a different impact on plant organs for each medicinal species studied