Geldanamycin and herbimycin A induce apoptotic killing of B chronic lymphocytic leukemia cells and augment the cells' sensitivity to cytotoxic drugs.

We studied the actions of geldanamycin (GA) and herbimycin A (HMA), inhibitors of the chaperone proteins Hsp90 and GRP94, on B chronic lymphocytic leukemia (CLL) cells in vitro. Both drugs induced apoptosis of the majority of CLL isolates studied. Whereas exposure to 4-hour pulses of 30 to 100 nM GA killed normal B lymphocytes and CLL cells with similar dose responses, T lymphocytes from healthy donors as well as those present in the CLL isolates were relatively resistant. GA, but not HMA, showed a modest cytoprotective effect toward CD34+ hematopoietic progenitors from normal bone marrow. The ability of bone marrow progenitors to form hematopoietic colonies was unaffected by pulse exposures to GA. Both GA and HMA synergized with chlorambucil and fludarabine in killing a subset of CLL isolates. GA- and HMA-induced apoptosis was preceded by the up-regulation of the stress-responsive chaperones Hsp70 and BiP. Both ansamycins also resulted in down-regulation of Akt protein kinase, a modulator of cell survival. The relative resistance of T lymphocytes and of CD34+ bone marrow progenitors to GA coupled with its ability to induce apoptosis following brief exposures and to synergize with cytotoxic drugs warrant further investigation of ansamycins as potential therapeutic agents in CLL

Does rotation of B stars depend on metallicity? preliminary results from GIRAFFE spectra

We show the vsini distribution of main sequence B stars in sites of various metallicities, in the absolute magnitude range -3.34 < Mv < -2.17. These include Galactic stars in the field measured by Abt et al. (2002), members of the h & chi Per open clusters measured by North et al. (2004), and five fields in the SMC and LMC measured at ESO Paranal with the FLAMES-GIRAFFE spectrograph, within the Geneva-Lausanne guaranteed time. Following the suggestion by Maeder et al. (1999), we do find a higher rate of rapid rotators in the Magellanic Clouds than in the Galaxy, but the vsini distribution is the same in the LMC and in the SMC in spite of their very different metallicities.Comment: 2 pages, 1 figure, poster presented at the ESO/Arcetri Workshop on "Chemical abundances and mixing in stars in the Milky Way and its satellites", 13-17 Sept. 200

Mental health promotion for young people - the case for yoga in schools

Peer reviewedPublisher PD

The early days of the Sculptor dwarf spheroidal galaxy

We present the high resolution spectroscopic study of five -3.9<=[Fe/H]<=-2.5 stars in the Local Group dwarf spheroidal, Sculptor, thereby doubling the number of stars with comparable observations in this metallicity range. We carry out a detailed analysis of the chemical abundances of alpha, iron peak, light and heavy elements, and draw comparisons with the Milky Way halo and the ultra faint dwarf stellar populations. We show that the bulk of the Sculptor metal-poor stars follows the same trends in abundance ratios versus metallicity as the Milky Way stars. This suggests similar early conditions of star formation and a high degree of homogeneity of the interstellar medium. We find an outlier to this main regime, which seems to miss the products of the most massive of the TypeII supernovae. In addition to its value to help refining galaxy formation models, this star provides clues to the production of cobalt and zinc. Two of our sample stars have low odd-to-even barium isotope abundance ratios, suggestive of a fair proportion of s-process; we discuss the implication for the nucleosynthetic origin of the neutron capture elements.Comment: Replacement after language editio

Definitive spectroscopic determination of the transverse interactions responsible for the magnetic quantum tunneling in Mn12-acetate

We present detailed angle-dependent single crystal electron paramagnetic resonance (EPR) data for field rotations in the hard plane of the S=10 single molecule magnet Mn12-acetate. A clear four-fold variation in the resonance positions may be attributed to an intrinsic fourth order transverse anisotropy (O44). Meanwhile, a four-fold variation of the EPR lineshapes confirms a recently proposed model wherein disorder associated with the acetic acid of crystallization induces a locally varying quadratic (rhombic) transverse anisotropy (O22). These findings explain most aspects of the magnetic quantum tunneling observed in Mn12-acetate.Comment: 7 pages, including figures, accepted for publication in Phys. Rev. Let

Mapping the complete glycoproteome of virion-derived HIV-1 gp120 provides insights into broadly neutralizing antibody binding

The surface envelope glycoprotein (SU) of Human immunodeficiency virus type 1 (HIV-1), gp120SU plays an essential role in virus binding to target CD4+ T-cells and is a major vaccine target. Gp120 has remarkably high levels of N-linked glycosylation and there is considerable evidence that this “glycan shield” can help protect the virus from antibody-mediated neutralization. In recent years, however, it has become clear that gp120 glycosylation can also be included in the targets of recognition by some of the most potent broadly neutralizing antibodies. Knowing the site-specific glycosylation of gp120 can facilitate the rational design of glycopeptide antigens for HIV vaccine development. While most prior studies have focused on glycan analysis of recombinant forms of gp120, here we report the first systematic glycosylation site analysis of gp120 derived from virions produced by infected T lymphoid cells and show that a single site is exclusively substituted with complex glycans. These results should help guide the design of vaccine immunogens

Analysis of clean coal technology in Nigeria for energy generation

Abstract: An analysis of clean coal technologies for the recovery of energy from Nigerian coals was carried out. The coal mines studied are Onyeama, Ogwashi, Ezimo, Inyi, Amasiodo, Okaba, Lafia-Obi, Owukpa Owukpa, Ogboyoga and Okpara. The estimated reserves of the ten coal deposit amount to 2.1 Gt, which is about 84 % of the total coal reserves of the country 2.5 Gt of coal Nigeria. The key clean coal technologies studied are Ultra-Supercritical Combustion (USC), Supercritical-Fluidised Bed Combustion (FBC), Integrated Gasification Combined Cycle (IGCC) and Coal bed Methane (CBM) and the results were compared with conventional subcritical pulverised fuel combustion (PF). The total potential energy recovery from these technologies are: PF 5800 TWh, FBC 7250 TWh, IGCC 7618 TWh, and USC 8519 TWh. This indicates an increase of about 31% in the total electricity generation if USC technology is used instead of the conventional sub-critical PF technology..

Understanding Face and Shame: A Servant-Leadership and Face Management Model

Clergy can have a negative impact on churches and other individuals when they knowingly or unknowingly attempt to save face, that is, try to protect their standing or reputation. The desire to gain face and the fear of losing face and feeling ashamed will likely permeate clergy’s decision-making processes without even being noticed. This study explores the essence of face and face management and the relationship between face management and two characteristics of servant-leadership—awareness and healing—in both Chinese and American churches through the methodology of hermeneutic phenomenology. Prior to this study, to my knowledge, no hermeneutic phenomenological research of face management has been conducted in a church setting. Through a review of the literature, four areas are explored: face and shame, face management, servant-leadership, and face, shame, and face management within the church. This study obtained approval from the Institutional Review Board and informed consent from the participants. Three Chinese and three American Christian ministers were chosen to complete a question sheet and participate in two semi-structured interview sessions. A first cycle of open coding and second cycle of pattern coding were used during data analysis. Face experiences are discussed in light of eight major themes: body, triggers, becoming, face concepts, strategies, emotions, servant-leadership, and the church. Findings from the study help build a servant-leadership and face management model, which can offer an anchored approach for clergy and pastoral counselors to address face and shame and to develop therapeutic interventions

Semiconductive and Photoconductive Properties of the Single Molecule Magnets Mn12_{12}-Acetate and Fe8_8Br8_8

Resistivity measurements are reported for single crystals of Mn$_{12}$-Acetate and Fe$_8$Br$_8$. Both materials exhibit a semiconductor-like, thermally activated behavior over the 200-300 K range. The activation energy, $E_a$, obtained for Mn$_{12}$-Acetate was 0.37 $\pm$ 0.05 eV, which is to be contrasted with the value of 0.55 eV deduced from the earlier reported absorption edge measurements and the range of 0.3-1 eV from intramolecular density of states calculations, assuming $2E_a$= $E_g$, the optical band gap. For Fe$_8$Br$_8$, $E_a$ was measured as 0.73 $\pm$ 0.1 eV, and is discussed in light of the available approximate band structure calculations. Some plausible pathways are indicated based on the crystal structures of both lattices. For Mn$_{12}$-Acetate, we also measured photoconductivity in the visible range; the conductivity increased by a factor of about eight on increasing the photon energy from 632.8 nm (red) to 488 nm (blue). X-ray irradiation increased the resistivity, but $E_a$ was insensitive to exposure.Comment: 7 pages, 8 figure

Perceived importance of components of asynchronous music in circuit training

This study examined regular exercisers’ perceptions of specific components of music during circuit training. Twenty-four men (38.8 years, s = 11.8 years) and 31 women (32.4 years, s = 9.6 years) completed two questionnaires immediately after a circuit training class. Participants rated the importance of 13 components of music (rhythm, melody, etc.) in relation to exercise enjoyment, and each completed the Affect Intensity Measure (Larsen, 1984) to measure emotional reactivity. Independent t tests were used to evaluate gender differences in perceptions of musical importance. Pearson correlations were computed to evaluate the relationships between affect intensity, age and importance of musical components. Consistent with previous research and theoretical predictions, rhythm response components (rhythm, tempo, beat) were rated as most important. Women rated the importance of melody significantly higher than did men, while men gave more importance to music associated with sport. Affect intensity was found to be positively and significantly related to the perceived importance of melody, lyrical content, musical style, personal associations and emotional content. Results suggest that exercise leaders need to be sensitive to personal factors when choosing music to accompany exercise. Qualitative research that focuses on the personal meaning of music is encouraged