Identificación de micobacterias atípicas aisladas de muestras clínicas en el Laboratorio Central de Salud Pública, Paraguay (2010-2013)

La micobacteriosis es una patología causada por micobacterias conocidas como atípicas (MA) o no MT (MNT), de complejo diagnóstico y tratamiento. De las casi 200 especies descriptas, alrededor de 25 son las más comúnmente involucradas como agentes infecciosos oportunistas. La aparición del VIH y los tratamientos estéticos invasivos, propiciaron su manifestación como enfermedad emergente en los últimos años. Se determinó la frecuencia de aislamiento de micobacterias atípicas en muestras clínicas y cepas ingresadas al Laboratorio Central de Salud Pública entre los años 2010 y 2013. De 2765 cultivos positivos para micobacterias, 171 aislamientos correspondían a micobacterias atípicas, las que fueron identificadas por la técnica molecular PRA-hsp65 (PCR Restriction Analysis-hsp65), y por secuenciación de los genes 16S rRNA, hsp-65 y rpo ? en los aislamientos que no pudieron identificarse por PRA-hsp65. La frecuencia total de aislamientos fue del 6,2%, correspondiendo a la siguiente distribución: M. intracellulare 25,9%, M. avium 25,9%, M. abscessus 13,3% y M. fortuitum 12,7%; el 17,1% restante involucra a 14 especies diferentes; y en 9 casos (5,1%) no se llegó a identificación. Fueron mayormente aisladas en muestras de origen pulmonar (88,9%); en la adultez (32,2%) y tercera edad (26,9%); en el sexo masculino (62,6%); así como en pacientes PVVS (12,3%). Los resultados obtenidos coinciden con las publicaciones regionales en cuanto a especies más frecuentemente aisladas, y coinfección con VIH. En cuanto al origen de la muestra, edad y sexo no existe un consenso general de relación con estas variables en las publicaciones

Detection, Subgroup Specificity, and Genotype Diversity of Rotavirus Strains in Children with Acute Diarrhea in Paraguay

Of a total of 220 stool specimens from children with acute diarrhea, mostly under the age of 3 years, collected in Paraguay between January 1999 and March 2000, 70 (31.8%) were found positive for rotaviruses (RV). Positive samples were characterized by electropherotyping and subgrouping. Sixty-one (87.1%) were classified as group A, subgroup II; one (1.4%) was classified as group A, subgroup I; six (8.6%) were group A, non-I non-II; and two (2.9%) were not tested. RV strains were G and P genotyped by reverse transcription-PCR. The following G types were detected: G4 (34.3%), G1 (21.4%), G2 (1.4%), and G9 (5.7%). Mixtures of human and animal genotypes were detected in 15 (21.4%) samples, and 11 samples (15.7%) were nontypeable. The following P types were detected: P[8] (48.6%), P[4] (1.4%), and P[1] (1.4%). A mixed type was found in 10% of samples, and an unexpectedly high percentage (38.6%) of nontypeable samples was found. The common human G- and P-type combinations P[8], G4 (15.7%) and P[8], G1 (14.2%) were detected. Mixed human and animal genotypes were observed as the following combinations: G4 + G5, G4 + G5 + G10, and G1 + G10 for G types and P[8]-P[1] for P types. The emerging G9 genotype was detected in four samples. These results show for the first time the diversity of RV circulating among children in Paraguay and contribute to the knowledge of this pathogen required to devise strategies to prevent diarrheal illness in this country. The finding of mixed genotypes may indicate interspecies transmission of RV between humans and animals.

First South american network of biomedical research. Education and biotechnology for health

Made available in DSpace on 2015-09-21T17:25:13Z (GMT). No. of bitstreams: 2 license.txt: 1914 bytes, checksum: 7d48279ffeed55da8dfe2f8e81f3b81f (MD5) wilson_savino_etal_IOC_2013.pdf: 221595 bytes, checksum: e75e4f7146bbef3d04e2d08e63acf897 (MD5) Previous issue date: 2013Instituto de Investigación en Biomedicina de Buenos Aires. CONICET-Partner de la Sociedad Max-Planck. Buenos Aires, Argentina.Intituto de Investigaciónes en Ciencias de la Salud (IICS). Paraguay.Centro para el Desarrollo de Investigación Científica (CEDIC). Paraguay.Laboratorio Central de Salud Pública del Ministerio de Salud (LCSP). ParaguayLaboratorio Central de Salud Pública del Ministerio de Salud (LCSP). ParaguayFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Institut Pasteur de Montevideo. (IP Montevideo), Uruguay.Instituto de Investigación en Biomedicina de Buenos Aires. CONICET-Partner de la Sociedad Max-Planck. Buenos Aires, Argentina.Se da a conocer la creación del primer programa de integración regional de una red de Institutos de Investigación en Biomedicina pertenecientes a países miembros del MERCOSUR. Se analizan las bases que dieron sustento a su creación y sus objetivos en el mediano y largo plazo. Además, se estima el potencial de los resultados de este programa en los campos de la investigación médica aplicada, educación y biotecnología.First South American Network of Biomedical Research. Education and Biotechnology for Health. It is in our interest, in this brief manuscript, to report the creation of the first program of regional integration of a network of research institutes in Biomedicine belonging to members of the MERCOSUR countries. We discuss some of the foundations that gave sustenance to its creation and its objectives in the medium and long term. In addition, we consider the potential of the results of this program in the fields of applied medical research, education and biotechnology

Primera Red Sudamericana de Biomedicina: Investigación, educación y biotecnología aplicadas a la salud

Se da a conocer la creación del primer programa de integración regional de una red de Institutos de Investigación en Biomedicina pertenecientes a países miembros del MERCOSUR. Se analizan las bases que dieron sustento a su creación y sus objetivos en el mediano y largo plazo. Además, se estima el potencial de los resultados de este programa en los campos de la investigación médica aplicada, educación y biotecnología

Dengue virus type 3 isolated from a fatal case with visceral complications induces enhanced proinflammatory responses and apoptosis of human dendritic cells

O artigo encontra-se disponível em acesso aberto no site do Editor.Submitted by Manoel Barata ([email protected]) on 2018-09-18T19:33:15Z No. of bitstreams: 1 JournalVirolj5374.pdf: 1684849 bytes, checksum: ad2c1970d09d920e97d0fac732573033 (MD5)Approved for entry into archive by Manoel Barata ([email protected]) on 2018-09-19T16:45:53Z (GMT) No. of bitstreams: 1 JournalVirolj5374.pdf: 1684849 bytes, checksum: ad2c1970d09d920e97d0fac732573033 (MD5)Made available in DSpace on 2018-09-19T16:45:53Z (GMT). No. of bitstreams: 1 JournalVirolj5374.pdf: 1684849 bytes, checksum: ad2c1970d09d920e97d0fac732573033 (MD5) Previous issue date: 2011Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil / Universidade de la Republica. Facultad de Ciencias. Sección Virologia, Montevideo, Uruguai.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Instituto de Previsión Social. Laboratorio Central de Salud Publica. Servicio de Pediatria, Asuncion, Paraguay.Instituto de Previsión Social. Laboratorio Central de Salud Publica. Servicio de Pediatria, Asuncion, Paraguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Universidade Federal de Santa Catarina. Laboratório de Imunofarmacologia e Doenças Infecciosas. Florianópolis, SC, BrazilFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.A recent (2007 to 2009) dengue outbreak caused by dengue virus (DENV) in Paraguay presented unusual severe clinical outcomes associated with 50% mortality rates. Although it has been reported that inflammatory responses influence the severity of dengue virus infection (T. Pang, M. J. Cardosa, and M. G. Guzman, Immunol. Cell Biol. 85:43-45, 2007), there remains a paucity of information on virus-innate immunity interactions influencing clinical outcome. Using human dendritic cells from a major innate immune cell population as an in vitro model, we have investigated signature cytokine responses as well as infectivity-replicative profiles of DENV clinical isolates from either a nonfatal case of classical dengue fever (strain DENV3/290; isolated in Brazil in 2002) or a fatal case of dengue fever with visceral complications isolated in Paraguay in 2007 (strain DENV3/5532). Strain DENV3/5532 was found to display significantly higher replicative ability than DENV3/290 in monocyte-derived dendritic cells (mdDCs). In addition, compared to DENV3/290 results, mdDCs exposed to DENV3/5532 showed increased production of proinflammatory cytokines associated with higher rates of programmed cell death, as shown by annexin V staining. The observed phenotype was due to viral replication, and tumor necrosis factor alpha (TNF-α) appears to exert a protective effect on virus-induced mdDC apoptosis. These results suggest that the DENV3/5532 strain isolated from the fatal case replicates within human dendritic cells, modulating cell survival and synthesis of inflammatory mediators

Retrospective Spatio-Temporal Dynamics of Dengue Virus 1, 2 and 4 in Paraguay

Dengue virus (DENV) has been a major public health concern in Paraguay, with frequent outbreaks occurring since early 1988. Although control measures have been implemented, dengue remains a significant health threat in the country, and continued efforts are required for prevention and control. In response to that, in collaboration with the Central Public Health Laboratory in Asunción, we conducted a portable whole-genome sequencing and phylodynamic analysis to investigate DENV viral strains circulating in Paraguay over the past epidemics. Our genomic surveillance activities revealed the co-circulation of multiple DENV serotypes: DENV-1 genotype V, the emerging DENV-2 genotype III, BR4-L2 clade, and DENV-4 genotype II. Results additionally highlight the possible role of Brazil as a source for the international dispersion of different viral strains to other countries in the Americas emphasizing the need for increased surveillance across the borders, for the early detection and response to outbreaks. This, in turn, emphasizes the critical role of genomic surveillance in monitoring and understanding arbovirus transmission and persistence locally and over long distances