12 research outputs found
Fundamental physics activities with pulsed neutron at J-PARC(BL05)
"Neutron Optics and Physics (NOP/ BL05)" at MLF in J-PARC is a beamline for
studies of fundamental physics. The beamline is divided into three branches so
that different experiments can be performed in parallel. These beam branches
are being used to develop a variety of new projects. We are developing an
experimental project to measure the neutron lifetime with total uncertainty of
1 s (0.1%). The neutron lifetime is an important parameter in elementary
particle and astrophysics. Thus far, the neutron lifetime has been measured by
several groups; however, different values are obtained from different
measurement methods. This experiment is using a method with different sources
of systematic uncertainty than measurements conducted to date. We are also
developing a source of pulsed ultra-cold neutrons (UCNs) produced from a
Doppler shifter are available at the unpolarized beam branch. We are developing
a time focusing device for UCNs, a so called "rebuncher", which can increase
UCN density from a pulsed UCN source. At the low divergence beam branch, an
experiment to search an unknown intermediate force with nanometer range is
performed by measuring the angular dependence of neutron scattering by noble
gases. Finally the beamline is also used for the research and development of
optical elements and detectors. For example, a position sensitive neutron
detector that uses emulsion to achieve sub-micrometer resolution is currently
under development. We have succeeded in detecting cold and ultra-cold neutrons
using the emulsion detector.Comment: 9 pages, 5 figures, Proceedings of International Conference on
Neutron Optics (NOP2017
Phosphoproteomics-Based Modeling Defines the Regulatory Mechanism Underlying Aberrant EGFR Signaling
BACKGROUND: Mutation of the epidermal growth factor receptor (EGFR) results in a discordant cell signaling, leading to the development of various diseases. However, the mechanism underlying the alteration of downstream signaling due to such mutation has not yet been completely understood at the system level. Here, we report a phosphoproteomics-based methodology for characterizing the regulatory mechanism underlying aberrant EGFR signaling using computational network modeling. METHODOLOGY/PRINCIPAL FINDINGS: Our phosphoproteomic analysis of the mutation at tyrosine 992 (Y992), one of the multifunctional docking sites of EGFR, revealed network-wide effects of the mutation on EGF signaling in a time-resolved manner. Computational modeling based on the temporal activation profiles enabled us to not only rediscover already-known protein interactions with Y992 and internalization property of mutated EGFR but also further gain model-driven insights into the effect of cellular content and the regulation of EGFR degradation. Our kinetic model also suggested critical reactions facilitating the reconstruction of the diverse effects of the mutation on phosphoproteome dynamics. CONCLUSIONS/SIGNIFICANCE: Our integrative approach provided a mechanistic description of the disorders of mutated EGFR signaling networks, which could facilitate the development of a systematic strategy toward controlling disease-related cell signaling
個々の患者に適応した処方監査 病院情報システムからの患者基本情報の抽出と処方監査への応用
出版社版薬剤や患者の特性に合わせたチェック項目を病院情報システムおよび疑義照会データベースより取得し、処方せんと同時にプリントアウト(チェックシート)できるようなシステムを開発した。チェックシートの導入による処方監査について評価・考察した。薬歴および基本検査値のチェックにおいて極めて便利であるとの評価が得られた。結果として、重複処方(他科での処方と)発見、腎・肝機能等の低下に注意すべき薬剤の処方変更などが効果として挙げられた