月下詠嘆とその構図 ―『伊勢物語』西の対の段における―

片桐洋一教授古稀記念特

Round Robin: A New Qualitative Methodology for Identifying Drivers of Violence Against Children

Violence affecting children (VAC) is a significant global health and human rights issue. This article highlights a new qualitative methodology, the Round Robin, for understanding the drivers of violence against children. Traditionally, qualitative research exploring VAC has focused on identifying the risk and protective factors which affect the likelihood a child will experience or witness violence. In recent years, scholars have recognised the need to situate children in their socio-cultural context and consider what causes risk and protective factors; that is, what drives violence at the structural and institutional levels of society. The Round Robin methodology sits within the participatory paradigm and contributes not only to the field of violence research, but to qualitative research more broadly, as it can be adapted to fit diverse social issues and contexts. The Round Robin combines focus groups and participatory techniques in an intensive three-day workshop model inspired by the World Café. In this paper, we firstly introduce the Round Robin methodology and situate it in relation to other approaches. We then describe and critique how the Round Robin methodology was piloted with 136 young people in Zimbabwe to identify drivers of violence affecting children. We then justify the methods used to collect data, and the strategy for data recording and analysis. We conclude by identifying the strengths and weaknesses we uncovered piloting this new methodology in Zimbabwe

Identification of protease serine S1 family member 53 as a mitochondrial protein in murine islet beta cells

The aim of this study was to identify genes that are specifically expressed in pancreatic islet β-cells (hereafter referred to as β-cells). Large-scale complementary DNA-sequencing analysis was performed for 3,429 expressed sequence tags derived from murine MIN6 β-cells, through homology comparisons using the GenBank database. Three individual ESTs were found to code for protease serine S1 family member 53 (Prss53). Prss53 mRNA is processed into both a short and long form, which encode 482 and 552 amino acids, respectively. Transient overexpression of myc-tagged Prss53 in COS-7 cells showed that Prss53 was strongly associated with the luminal surfaces of organellar membranes and that it underwent signal peptide cleavage and N-glycosylation. Immunoelectron microscopy and western blotting revealed that Prss53 localized to mitochondria in MIN6 cells. Short hairpin RNA-mediated Prss53 knockdown resulted in Ppargc1a downregulation and Ucp2 and Glut2 upregulation. JC-1 staining revealed that the mitochondria were depolarized in Prss53-knockdown MIN6 cells; however, no change was observed in glucose-stimulated insulin secretion. Our results suggest that mitochondrial Prss53 expression plays an important role in maintaining the health of β-cells

Relationship between peak oxygen uptake and regional body composition in Japanese subjects

AbstractPurposeTo investigate the link between peak oxygen uptake and regional body composition by dual energy X-ray absorptiometry (DEXA) in Japanese subjects.MethodsA total of 93 men (42.2 ± 12.3 years old) and 106 women (43.5 ± 12.3 years old) were enrolled in this cross-sectional investigation study. Peak oxygen uptake was measured by the breath-by-breath method. Regional body composition i.e., body fat mass, lean body mass, and body fat percentage was evaluated using DEXA. In addition, metabolic risk parameters were also evaluated.ResultsPeak oxygen uptake was 37.6 ± 8.7 mL/kg/min in men and 31.1 ± 6.4 mL/kg/min in women, and decreased with age in both genders. Peak oxygen uptake was significantly correlated with total body fat percentage (men: r = −0.684, p < 0.0001; women: r = −0.681, p < 0.0001). These associations remained even after adjusting for age and total lean body mass. However, peak work rate was positively and significantly correlated with leg lean body mass.ConclusionPeak oxygen uptake was closely correlated with total body fat percentage in both genders. Aerobic exercise as well as leg resistance training might be useful for improving peak oxygen uptake in Japanese subjects

Interdisciplinary Class on the Climate Environment around the Japan Islands in Association with the Seasonal Feeling (with Attention to the Transition Stage from Autumn to Winter)

The complicated seasonal variations are found in East Asia influenced by the Asian monsoon, resulting in the variety of “seasonal feeling”. For example, although the air temperature around the Japan Islands is still rather higher from November to early December than in the midwinter, the wintertime weather pattern often appears then due to the development of the Siberian high. In the Hokuriku District, the Japan Sea side of the central Japan, the shallow convective rainfall called the “Shi-gu-re” frequently occurs associated with the air mass transformation process over the Japan Sea in the cold air outbreak situation from the continent. It is also well known that the “Shi-gu-re” is often used for expression of the “seasonal feeling” in the Japanese classical literature. Thus the present study tried to develop an interdisciplinary class on the climate environment around the Japan Islands in association with the “seasonal feeling”, with attention to the transition stage from autumn to winter. The present paper will discuss on the joint activity of meteorology with the Japanese classical literature, the music and the art, for the class at the Faculty of Education, Okayama University, and that at the Okayama-Ichinomiya High School