2 research outputs found
Investigation of the Peptide Adsorption on ZrO<sub>2</sub>, TiZr, and TiO<sub>2</sub> Surfaces as a Method for Surface Modification
Specific surface binding peptides
offer a versatile and interesting possibility for the development
of biocompatible implant materials. Therefore, eight peptide sequences
were examined in regard to their adsorption on zirconium oxide (ZrO<sub>2</sub>), titanium zircon (TiZr), and titanium (c.p. Ti). Surface
plasmon resonance (SPR) measurements were performed on Ti coated sensor
chips to determine the kinetics of the interactions and kinetic rate
constants (<i>k</i><sub>on</sub>, <i>k</i><sub>off</sub>, <i>K</i><sub>D</sub>, and <i>R</i><sub>max</sub>). We also investigated the interactions which are
present in our system. Electrostatic and coordinative interactions
were found to play a major role in the adsorption process. Four of
the eight examined peptide sequences showed a significant adsorption
on all investigated materials. Moreover, the two peptides with the
highest adsorption could be quantified (up to 370 pmol/cm<sup>2</sup>). For potential biomaterials applications, we proved the stability
of the adsorption of selected peptides in cell culture media, under
competition with proteins and at body temperature (37 °C), and
their biocompatibility via their effects on the adhesion and proliferation
of human mesenchymal stem cells (hMSCs). The results qualify the peptides
as anchor peptides for the biofunctionalization of implants