Metabolic transcription analysis of engineered strains that overproduce L-phenylalanine-3

<p><b>Copyright information:</b></p><p>Taken from "Metabolic transcription analysis of engineered strains that overproduce L-phenylalanine"</p><p>http://www.microbialcellfactories.com/content/6/1/30</p><p>Microbial Cell Factories 2007;6():30-30.</p><p>Published online 19 Sep 2007</p><p>PMCID:PMC2089068.</p><p></p> carbon central metabolism, fermentative pathways and the connection with the common aromatic amino acid pathway. Most relevant transcriptional responses in host strains without plasmids are shown: PB12 (first value), PB13 (second value) as compared to JM101. According to the significance criterion, only those relative gene transcription values ≥ 2 (up-regulation, data in red) or ≤ 0.5 (down-regulation, data in blue), as compared to JM101 reference strain, are shown. The relative gene transcription value for JM101 is always equal to 1 and for that reason was omitted. No significant values were written in black. Metabolites abbreviations: GLC, glucose; G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; PBP, fructose-1,6-biphosphate; DHAP, dihydroxyacetone phosphate; GAP, glyceraldehyde 3-phosphate; 1,3-BGP, 1,3-biphosphoglycerate; 3PG, 3-phosphoglycerate; 2PG, 2-phophoglycerate; PEP, phosphoenolpyruvate; PYR, pyruvate; 6PGLN, 6-phosphoglucono-δ-lactone; 6PGNT, 6-phophogluconate; Ru5P, ribulose-5-phosphate; R5P, ribose-5-phosphate; Xu5P, xylulose-5-phosphate; S7P, sedoheptulose-7-phosphate; E4P, erythrose-4-phosphate; Ac-CoA, acetyl coenzyme A; Ac-P, acetyl phosphate; Ac-AMP, acetyl-AMP; CIT, citrate; ICT, isocitrate; GOX, glyoxylate; α-KG, α-ketoglutarate; SUC-CoA, succinyl-coenzyme A, SUC, succinate; FUM, fumarate; MAL, malate; OXA, oxaloacetate

Metabolic transcription analysis of engineered strains that overproduce L-phenylalanine-4

<p><b>Copyright information:</b></p><p>Taken from "Metabolic transcription analysis of engineered strains that overproduce L-phenylalanine"</p><p>http://www.microbialcellfactories.com/content/6/1/30</p><p>Microbial Cell Factories 2007;6():30-30.</p><p>Published online 19 Sep 2007</p><p>PMCID:PMC2089068.</p><p></p> carbon central metabolism, fermentative pathways and the connection with the common aromatic amino acid pathway. Most relevant transcriptional responses in L-Phe overproducing strains are shown: JM101-ev2 (first value), PB12-ev2 (second value) and PB13-ev3 (third value), as compared to JM101. Metabolites abbreviations are depicted in Figure 3 legend

Metabolic transcription analysis of engineered strains that overproduce L-phenylalanine-0

<p><b>Copyright information:</b></p><p>Taken from "Metabolic transcription analysis of engineered strains that overproduce L-phenylalanine"</p><p>http://www.microbialcellfactories.com/content/6/1/30</p><p>Microbial Cell Factories 2007;6():30-30.</p><p>Published online 19 Sep 2007</p><p>PMCID:PMC2089068.</p><p></p> and aromatic amino acid pathways are shown

Metabolic transcription analysis of engineered strains that overproduce L-phenylalanine-2

<p><b>Copyright information:</b></p><p>Taken from "Metabolic transcription analysis of engineered strains that overproduce L-phenylalanine"</p><p>http://www.microbialcellfactories.com/content/6/1/30</p><p>Microbial Cell Factories 2007;6():30-30.</p><p>Published online 19 Sep 2007</p><p>PMCID:PMC2089068.</p><p></p>aromatic amino acid pathways. Metabolites abbreviations: PEP, phosphoenolpyruvate; E4P, erythrose 4-phosphate; DAHP, 3-deoxy-D--heptulosonate-7-phosphate; DHQ, 5-dehydroquinate; DHS, 5-dehydroshikimate; SHIK, shikimate; S3P, shikimate 5-phosphate; ESPS, 3-enolpyruvylshimate-5-phosphate; CHO, chorismate; PPA, prephenate; PPY, phenylpyruvate; HPP, 4-hydroxyphenylpyruvate; ANTA, anthranilate; PRAA, N-(5'-Phosphoribosyl)-anthranilate; CDRP, enol-1-o-carboxyphenylamino-1-deoxy-ribulose phosphate; I3GP, indol-3-glycerol phosphate; IND, indole; L-Phe, phenylalanine; L-Tyr, tyrosine; L-Trp, tryptophan, L-Ser, serine; L-Gln, glutamine; L-Glu, glutamate

Metabolic transcription analysis of engineered strains that overproduce L-phenylalanine-1

<p><b>Copyright information:</b></p><p>Taken from "Metabolic transcription analysis of engineered strains that overproduce L-phenylalanine"</p><p>http://www.microbialcellfactories.com/content/6/1/30</p><p>Microbial Cell Factories 2007;6():30-30.</p><p>Published online 19 Sep 2007</p><p>PMCID:PMC2089068.</p><p></p> carbon central metabolism, fermentative pathways and the connection with the common aromatic amino acid pathway. Most relevant transcriptional responses in L-Phe overproducing strains are shown: JM101-ev2 (first value), PB12-ev2 (second value) and PB13-ev3 (third value), as compared to JM101. Metabolites abbreviations are depicted in Figure 3 legend

Characterization of C and ethanologenic strains CCE14 and KO11 in M9 mineral media with 40 g/l glucose

Cell mass formation (A), Glucose (B), Pyruvate (C), Formate (D), Ethanol (E), Acetate (F), Lactate (G), and Succinate (H).<p><b>Copyright information:</b></p><p>Taken from "Metabolic regulation analysis of an ethanologenic strain based on RT-PCR and enzymatic activities"</p><p>http://www.biotechnologyforbiofuels.com/content/1/1/8</p><p>Biotechnology for Biofuels 2008;1():8-8.</p><p>Published online 1 May 2008</p><p>PMCID:PMC2396614.</p><p></p

Specific enzyme activities values of PDCand ADHfor strains KO11 and CCE14 during exponential (A) and stationary phases (B)

<p><b>Copyright information:</b></p><p>Taken from "Metabolic regulation analysis of an ethanologenic strain based on RT-PCR and enzymatic activities"</p><p>http://www.biotechnologyforbiofuels.com/content/1/1/8</p><p>Biotechnology for Biofuels 2008;1():8-8.</p><p>Published online 1 May 2008</p><p>PMCID:PMC2396614.</p><p></p

RT-PCR values for strain CCE14 normalized to C during the exponential phase

Higher values are represented in a continuous borderline and lower values are in dotted borderline. A -student test with a value of ≤ 0.05 was applied to each set of normalized values in order to determinate statistical differences in expression levels. Glucose transporter protein EIICB(), Glucokinase (), Phosphoglucose isomerase (), Phosphofructokinase (), Fructose bisphosphate aldolase (), Triose Phosphate isomerase (), Glyceraldehyde-3P dehydrogenase (), Phosphoglycerate kinase (), Phosphoglycerate mutase (), Enolase (), Pyruvate kinase A (), Pyruvate kinase B (), Glucose-6P-1-dehydrogenase (), 6-Phosphogluconate dehydrogenase (), Ribulose phosphate epimerase (), Ribose-5-phosphate isomerase A (), Ribose-5-phosphate isomerase B (), Transketolase A (), Transketolase B (), Transaldolase A (), Transaldolase B (), 2-keto-3-deoxy-phosphogluconate aldolase (), Phosphogluconate dehydratase (), Xylulose-5-phosphate (Xyl 5P), Ribulose-5-phosphate (Ril 5P), Glyceldehyde-3-phosphate (Gly 3P), Sedoheptulose-7-phosphate (S 7P), Erytrose 4-phosphate (Ery 4P), Fructose 6-phosphate (Fru 6P), 2-keto-3-deoxy-gluconate-6-phosphate (KDPGNT), Fumarate reductase A (), Fumarate reductase B (), Fumarate reductase C (), Fumarate reductase D (), pyruvate formate lyase B (), pyruvate formate lyase D (), Malate dehydrogenase (), Fumarase A (), Fumarase B (), Fumarase C (), alcohol dehydrogenase (), lactate dehydrogenase (), Transcriptional regulator CRP (), Transcriptional repressor MLC (), Transcriptional repressor MLC (), Transcriptional regulator FRUR (), High affinity maltose receptor (), Galactose ABC transporter (), Galactose permease (), alcohol dehydrogenase (), pyruvate dehydrogenase ().<p><b>Copyright information:</b></p><p>Taken from "Metabolic regulation analysis of an ethanologenic strain based on RT-PCR and enzymatic activities"</p><p>http://www.biotechnologyforbiofuels.com/content/1/1/8</p><p>Biotechnology for Biofuels 2008;1():8-8.</p><p>Published online 1 May 2008</p><p>PMCID:PMC2396614.</p><p></p

MOESM2 of Deletion of the 2-acyl-glycerophosphoethanolamine cycle improve glucose metabolism in Escherichia coli strains employed for overproduction of aromatic compounds

Additional file 2: Table S2. Mutation rates in both evolved strains based on mutation appearance in the rpoB locus. Mutation rates were determined by a modified Luria-Delbrück fluctuation test, employing the MSS-MLE and the LC methods in the estimation of the number of mutants [30–32]. The mutation frequency also was determinated