学会抄録

A description of the results of the cross platform (385 K CGH and SNP50 chip) verification of CNV regions [72, 73]. (PDF 8 kb

Additional file 6: of Identification of genes directly responding to DLK1 signaling in Callipyge sheep

Cellular localization of DNTTIP1. C2C12 cells were transfected with pDNTTIP1-pcDNA3.2 /V5 construct and stained with anti-DNTTIP1 antibody (red), anti-V5 epitope tag antibody (green) and DAPI (nuclei, blue). The merged cells (orange) showed the detection of the same cells by anti-DNTTIP1 and anti-V5 antibodies. DNTTIP1 localized exclusively in nucleus in C2C12 cells. (PDF 43 kb

Additional file 1: of Identification of genes directly responding to DLK1 signaling in Callipyge sheep

Quantitative PCR primer sequences and amplification conditions. (XLSX 13 kb

Additional file 5: of Identification of genes directly responding to DLK1 signaling in Callipyge sheep

Transcript abundance of RPLP0 in 7 muscles. Least square means and standard errors for log transcript abundance are shown for each muscle and genotype, callipyge (+/C) and normal (+/+). The hypertrophied muscles are LD, SM, ST, and TB and the non-hypertrophied muscles are SS, IS and HT. RPLP0 was used as a control to confirm equivalent RNA input into cDNA synthesis and qPCR assays. No significant differences were detected between the two genotypes in all the muscles analyzed. (PDF 21 kb

Additional file 2: of Identification of genes directly responding to DLK1 signaling in Callipyge sheep

The plasmids compositions of effector luciferase assay. (XLSX 9 kb

Additional file 3: of Identification of genes directly responding to DLK1 signaling in Callipyge sheep

Quantitative analysis of gene expression in 7 muscles (log). (XLSX 16 kb

Additional file 4: of Identification of genes directly responding to DLK1 signaling in Callipyge sheep

Analysis of variance of genotype effects in qPCR analysis. (XLSX 14 kb

Additional file 8: of Identification of genes directly responding to DLK1 signaling in Callipyge sheep

Cellular localization of METTL21E and GW-CAT. C2C12 cells were transfected with pMETTL21E-pcDNA3.2 /V5 construct (A to C) or pGWCAT- pcDNA3.2/V5 (D to F) and stained with anti-METTL21E or anti-GW-CAT antibody (green), and DAPI (nuclei, blue). METTL21E (A to C) localized both in the cytoplasm and nucleus but mostly accumulated in cytoplasm in C2C12 cells. GW-CAT (D to F) localized mostly in nucleus. (PDF 51 kb

Population divergence measured as <i>F</i>_ST (%).

1<p>The geographic region of breed development is given as SE (Southern Europe), NE (Northern Europe), (CE) Continental Europe or A (Asia).</p>2<p><i>F</i>_ST (given as a percentage) is given above the diagonal and its standard deviation (SD) for each combination is given below (x1000).</p

Genetic relationship between Gulf Coast Native and 12 other breeds.

<p>Individuals were clustered using PCA of allele sharing and plotted for PCA1 and 2 (<b>A</b>) and PCA1 and 6 (<b>B</b>). Individuals from different breeds are given using different colored symbols as follows: Gulf Coast Native (GCN gold), Merino (MER dark blue), Poll Dorset (APD dark green), Poll Merino (APM red), Castellana (CAS brown), Churra (CHU pink), Meat Lacaune (MEL light blue), Milk Lacaune (MIL black), Ojalada (OJA orange), Rambouillet (RAM purple), Rasa Aragonesa (RAS pale green), Sumatra (SUN violet) and Tibetan (TIB yellow). The two largest principal components (<b>A</b>) separated Asian (SUM, TIB) and Northern European animals (APD) from a cluster containing breeds developed in southern Europe (including RAM, MER and APM). Plotting PCA1 and PCA6 revealed the genetic division within GCN (<b>B</b>).</p