Additional file 1: of Whole-genome analysis of human papillomavirus genotypes 52 and 58 isolated from Japanese women with cervical intraepithelial neoplasia and invasive cervical cancer

Lineage/sublineage-specific SNPs of HPV52/58. (DOCX 53 kb

Comparison between DNA and RNA of HPV E6/E7 and human β2-microglobulin (B2M) genes.

<p>The size of E6/E7 DNA is 652bp for HeLa and 622bp for CaSki, same as E6 mRNA. The size of B2M DNA is 775bp and B2M RNA is 148bp.</p

E6/E7 mRNA analyses and HPV genotyping in LBC samples from patients with cervical neoplastic diseases.

<p>E6/E7 mRNA analyses and HPV genotyping in LBC samples from patients with cervical neoplastic diseases.</p

Kaplan-Meier curves for upgraded Pap-test results in followed-up patients with CIN1-2.

<p><i>A</i>, cases positive for both E6 and E7 mRNAs (n = 3) <i>vs</i>. the remainder (n = 28); <i>B</i>, cases with positive E7 mRNAs (n = 4) <i>vs</i>. negative E7 mRNAs (n = 27); <i>C</i>, cases with positive E6 mRNA (n = 15) <i>vs</i>. negative E6 mRNA (n = 16); <i>D</i>, cases with positive HR-HPV DNA (n = 26) <i>vs</i>. negative HR-HPV DNA (n = 5); <i>E</i>, cases with positive HPV16/18 DNA (n = 8) vs. negative HPV16/18 DNA (n = 23).</p

Study design.

<p>HPV: human papillomavirus, CIN: cervical intraepithelial neoplasia, ICC: invasive cervical cancer.</p

Detection of E6/E7 mRNAs from patients.

<p>E6 transcript is detected in samples 1 and 3, and 2 kinds of E7 transcript are detected in samples 2 and 3.</p

Relationship between E6/E7 mRNAs and HPV genotypes.

<p>Relationship between E6/E7 mRNAs and HPV genotypes.</p

E6/E7 mRNA expression patterns by RT-PCR in human cervical cancer cell lines.

<p>H: full-length E6/E7 (E6), M: spliced E6*I/E7 (E7), L: spliced E6*II/E7 (E7).</p