6 research outputs found
Embryogenic Protoplast Cultures of Orange Jessamine (Murraya Paniculata) and Their Regeneration Into Plants Flowering in Vitro
Embryogenic callus was induced from the hypocotyl region of seedlings germinated from immature embryos
of orange jessamine (Murraya paniculata (L.) Jack) on Murashige & Tucker (1969) medium containing 50 g 1-1
sucrose, 5.0 mg 1- l benzyladenine, 2.5 mg 1-1 2,4-dichlorophenoxyacetic acid and 600 mg 1-1 malt extract. Isolated protoplasts divided to produce callus on Murashige & Tucker (1969) medium containing 50 g 1-1 sucrose, 0.01
mg 1-1 gibberellin A4+7 and 600 mg 1-l malt extract. Callus developed to plantlets via somatic embryogenesis on
Murashige & Tucker (1969) medium with 50 g 1 -l lactose but no plant growth regulators. These plantlets flowered
in vitro on half strength Murashige & Tucker (1969) medium containing 50 g 1-l sucrose after 2 months culture
In vitro flowering of Fortunella hindsii (Champ.)
Abstract. Branch internodes of mature plants and stem
internodes of seedlings of Fortunella hindsii flowered in
vitro on half-strength MT (Murashige and Tucker 1969)
basal medium supplemented with benzyladenine, adenine,
6-~-~dimethylallylaminopurine and kinetin. The highest
percentage of flowering was achieved with explants ori-
ginating from branch internodes of flowering plants close
to the apex on half-strength MT basal medium containing
5% sucrose and 0.01 mg 1-1 BA in light. Exposure to
darkness for more than 3 weeks followed by re-exposure
to light reduced flowering. Flowering required a 4-day
exposure to BA, but shoot formation could be initiated
even without exposure to BA. First branch internode
segments on MT basal medium containing 5% sucrose
were prolific in flower (85%) production. The sucrose
treatment affected the flower bud size distribution. There
were about 13 flower buds per culture in the largest size
category (>5 ram)
Plant Regeneration Via Somatic Embryogenesis From Protoplasts Of Uganda Cherry Orange (Citropsis Schweinfurthii)
Protoplasts isolated from embryogenic callus of
Citropsis schweinfurthii (Engl.) Swing. & M. Kell were
cultured in MT (Murashige and Tucker 1969) basal
medium containing 5% sucrose supplemented with 0.0,
0.001, 0.01, 0.1 or 1.0 mg 1-1 BA, 0, 300, 600 or 900 mg
1-1 malt extract and 0.6 M sorbitol. The highest plating
efficiency was obtained on MT basal medium containing
5% sucrose supplemented with 0.01 mg 1-1 BA and 600
mg 1-1 malt extract. MT basal medium containing 5%
sucrose and supplemented with 0.01 mg 1-1 kinetin was
found to be a medium suitable for the development of
globular somatic embryos derived from protoplasts into
heart-shaped somatic embryos with cotyledon-like struc-
tures. The highest percentage of shoot formation was
obtained using 0.1 mg 1-1 GA 3. A complete protoplast-
to-plant system was developed for C. schweinfurthii,
which could facilitate the transfer of nuclear and cyto-
plasmic genes from this species into cultivated Citrus
through protoplast fusion
Plant Regeneration Via somatic Embryogenesis from Protoplasts of Six Plant Species Related to Citrus
Protoplasts isolated from embryogenic callus of
Fortunella polyandra (Ridl.), Atalantia bilocularis (Pieree
ex Guill.), Hesperethusa crenulata (Roxb.), Glycosmis
pentaphylla (Re~.) Ca)rr., Triphasia trifolia (Burm. f.) P.
Wils. and Murraya koenigii (L.) Spreng. were cultured in
MT (Murashige and Tucker 1969) basal medium contain-
ing 5% sucrose supplemented with 0.0, 0.001, 0.01, 0.1 or
1.0 mg 1-1 BA and 0.6 M sorbitol. The highest plating
efficiencies for all species were obtained on MT basal
medium containing 5% sucrose supplemented with 0.001
mg 1 -a B.A. E polyandra produced higher percentages of
globular ,somatic embryo development, while A. bilocu-
laris consistently showed a lower percentage of globular
somatic embryo development in all 5 concentrations of
BA. MT basal medium containing 5% sucrose and sup-
plemented with 0.001 mg 1-1 BA was found to be a suit-
able medium for development of globular somatic em-
bryos derived from protoplasts to form heart-shaped
somatic embryos with cotyledon-like structures. The
highest percentages of shoot fl)rmation for all 6 species
were obtained using 0.1 mg 1 -] GA. 3. A complete proto-
plast-to-plant system was developed for E polyandra, A.
bilocularis and Z trifolia, which could facilitate the trans-
fer of nuclear and cytoplasmic genes from these species
into cultivated Citrus through protoplast fusion