The percentages of people who had the saving appearance responses (SARs).

<p>MCI: mild cognitive impairment; AD: Alzheimer’s disease; DLB: dementia with Lewy bodies; *: <i>P</i> < 0.05 with Bonfferoni correction.</p

Results of logistic regression analysis for the relationship between saving appearance responses and AD in people with AD and MCI.

<p>Results of logistic regression analysis for the relationship between saving appearance responses and AD in people with AD and MCI.</p

Results of logistic regression analysis for the relationship between saving appearance responses and AD in people with AD and DLB.

<p>Results of logistic regression analysis for the relationship between saving appearance responses and AD in people with AD and DLB.</p

Demographics of participants.

<p>Demographics of participants.</p

Effect of miR-222 overexpression on insulin signaling in primary mouse hepatocytes.

<p>(A) Primary hepatocytes were transfected with 30 nM negative control oligos (control) or miR-222 mimic using HilyMax. After 2 days of transfection, miR-222 overexpression in the cells was confirmed by qRT-PCR (n = 6 per group). (B) Cells overexpressing miR-222 were treated with insulin (100 nM) for 10 min. Cells were harvested, and protein levels involved in insulin signaling were determined by WB. The IRS-1 and P-Akt levels were quantified by normalization with β-actin and total Akt. The values are expressed as mean ± SD from 4 independent experiments. (C) <i>Irs-1</i> and <i>Irs-2</i> mRNA expression in the cells overexpressing miR-222 were analyzed by qRT-PCR. (D) Each mRNA involved in insulin signaling was analyzed in the cells overexpressing miR-222 (n = 10 per group). Data are presented as means ± SD. *<i>p</i> < 0.05, **<i>p</i> < 0.01 compared with the control group.</p

Effect of miR-222 overexpression in HuH-7 cells.

<p>(A) HuH-7 cells were transfected with 30 nM of the negative control or miR-222 mimic. After 2 days of transfection, miR-222 overexpression was confirmed by qRT-PCR (n = 6 per group). (B) Cells overexpressing miR-222 were treated with insulin (100 nM) for 10 min. Cells were harvested, and protein levels involved in insulin signaling were determined with WB. The IRS-1 and P-Akt levels were quantified by normalization with β-actin and total Akt. The values are expressed as the mean ± SD from 4 independent experiments. (C) The pmirGLO-based 3' UTR reporter vector consisted of luciferase cDNA followed by the 3' UTR of the WT or Mut human <i>IRS-1</i> mRNA. (D) HEK-293 cells were co-transfected with the luciferase reporter construct containing WT or Mut 3' UTR of human <i>IRS-1</i> and the miR-222 mimic or the negative control oligos. After 2 days of treatment, a dual-luciferase assay of these cells was measured (n = 10 per group). Data are presented as mean ± SD. *<i>p</i> < 0.05, **<i>p</i> < 0.01 compared with the control group.</p

Effect of miR-222 overexpression on insulin signaling in primary mouse hepatocytes.

<p>(A) Primary hepatocytes were transfected with 30 nM negative control oligos (control) or miR-222 mimic using HilyMax. After 2 days of transfection, miR-222 overexpression in the cells was confirmed by qRT-PCR (n = 6 per group). (B) Cells overexpressing miR-222 were treated with insulin (100 nM) for 10 min. Cells were harvested, and protein levels involved in insulin signaling were determined by WB. The IRS-1 and P-Akt levels were quantified by normalization with β-actin and total Akt. The values are expressed as mean ± SD from 4 independent experiments. (C) <i>Irs-1</i> and <i>Irs-2</i> mRNA expression in the cells overexpressing miR-222 were analyzed by qRT-PCR. (D) Each mRNA involved in insulin signaling was analyzed in the cells overexpressing miR-222 (n = 10 per group). Data are presented as means ± SD. *<i>p</i> < 0.05, **<i>p</i> < 0.01 compared with the control group.</p

miR-222 expression is up-regulated in the livers of G+HFHSD-fed mice.

<p>(A) Body weight was measured during each treatment in NC or G+HFHSD-fed mice (n = 7–8 per group). (B, C) Fasting blood glucose and insulin levels were measured after 24 weeks of treatment. (D) HOMA-IR was calculated using fasting blood glucose and insulin levels after 24 weeks of treatment. (E, F) After 24 weeks of treatment, microRNA was collected from the livers of these mice. miR-222 and miR-221 expression were analyzed by qRT-PCR (n = 8 per group). (G) The proteins in the livers of these mice were analyzed with WB. The IRS-1 and IRS-2 levels were quantified by normalization with β-actin. (n = 4 per group). (H) <i>Irs-1</i> mRNA expression in the livers of these mice were analyzed by qRT-PCR. Data are presented as mean ± SD. *<i>p</i> < 0.05, **<i>p</i> < 0.01 compared with NC-fed mice.</p