Bounds on Compactness for LMXB Neutron Stars from X-ray Burst Oscillations

We have modelled X-ray burst oscillations observed with the Rossi X-ray Timing Explorer (RXTE) from two low mass X-ray binaries (LMXB): 4U 1636-53 with a frequency of 580 Hz, and 4U 1728-34 at a frequency of 363 Hz. We have computed least squares fits to the oscillations observed during the rising phase of bursts using a model which includes emission from either a single circular hot spot or a pair of circular antipodal hot spots on the surface of a neutron star. We model the spreading of the thermonuclear hot spots by assuming that the hot spot angular size grows linearly with time. We calculate the flux as a function of rotational phase from the hot spots and take into account photon deflection in the relativistic gravitational field of the neutron star assuming the exterior spacetime is the Schwarzschild metric. We find acceptable fits with our model and we use these to place constraints on the compactness of the neutron stars in these sources. For 4U 1636-53, in which detection of a 290 Hz sub-harmonic supports the two spot model, we find that the compactness (i.e., mass/radius ratio) is constrained to be M/R < 0.163 at 90% confidence (G = c = 1). This requires a relatively stiff equation of state (EOS) for the stellar interior. For example, if the neutron star has a mass of 1.4 Msun then its radius must be > 12.8 km. Fits using a single hot spot model are not as highly constraining. We discuss the implications of our findings for recent efforts to calculate the EOS of dense nucleon matter and the structure of neutron stars.Comment: 27 pages, 6 figures, AASTeX. Revised and expanded version. Resubmitted to Astrophysical Journa

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Gaseous abundances and methane supersaturation in Titan\u27s troposphere

Various properties of Titan\u27s troposphere are inferred from an analysis of Voyager 1 infrared spectrometer (IRIS) data between 200 and 600 cm-1. Two homogeneous spectral averages acquired at widely separated emission angles are chosen for the analysis. Both data sets are associated with northern low latitudes very close to that of the radio science ingress occultation point. Solutions require simultaneous nonlinear least-squares fits to the two IRIS data sets, coupled with iteration of the radio occultation refractivity data. Values and associated 1-σ uncertainties of several parameters are inferred from our analysis. These include mole fractions for molecular hydrogen (∼0.0011), argon (small), and methane near the surface (∼0.057). Solutions are also obtained for the hydrogen para-fraction (close to equilibrium, with considerable uncertainty), air temperature near the surface (∼93 K), surface temperature discontinuity (∼1 K), and maximum degree of methane supersaturation in the upper troposphere (∼1.5). Actual values for the above-mentioned parameters depend on the amount of ethane cloud near the tropopause. There is no evidence for methane clouds in the upper troposphere, nor is their presence compatible with large degrees of supersaturation. A wave number dependence for the stratospheric haze opacity is inferred similar to that found for a polymeric residue created in laboratory discharge experiments. This haze appears to be uniformly distributed with latitude between altitudes of 40 and 160 km, provided those nighttime data at southern high latitudes that are subject to possible systematic calibration errors are discounted. Assuming uniform haze distribution, both the air temperature and methane vapor mole fraction near the surface are symmetrically distributed about the equator, with lower values at higher latitudes. Either the tropopause temperature or the maximum degree of methane supersaturation is asymmetrically distributed about the equator. In either case, the data are consistent with a decrease of methane supersaturation toward the poles, which suggests an increase in mean annual precipitation at high latitudes compared with the equatorial region. If methane vapor is in saturation equilibrium with Titan\u27s surface, the derived latitudinal gradient of the near surface methane vapor mole fraction implies that the liquid content of the surface is ethane-enriched near the poles. Published by Elsevier Science Ltd