Frequencies (Fq) and absolute value (N) of coding HLA-G alleles, 5′URR and 3′UTR SNPs in the Malian population.

<p>Results are compared with previously published results on VBMD <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0082517#pone.0082517-DiCristofaro1" target="_blank">[41]</a>. Statistical differences between the two populations frequencies were measured using a chi-squared test (NS: not significant; *: p<0.05; **: p<0.01; ***: p<0.001).</p

Two-locus linkage disequilibrium (LD) in the Malian population (n = 230) between polymorphisms in the 5′URR (−725 C/G or T; −716 G/T; −201 G/A and −56 C/T), in the coding region (codons 13C/T; 31 A/T; 54 A/G; 110 C/A; 130 C/T; 159 T/C; 219 C/T and 258 C/T) and in the 3′UTR (<i>ins/del</i> exon 8; 3142 C/G; 3187 G/A and 3196 C/G).

<p>Statistical significance (p value at junction between two loci) is indicated by color (light gray: p<0.05, gray: p<0.01 and black p<0.001).</p

Correlation between sHLA-G levels and age in women.

<p>Statistical correlation between age and sHLA-G levels was tested using Spearman's rank test (rS = −0.1905, p = 0.004).</p

Comparison between UTR-2 alleles G*01:05N/G*01:05N (n = 5 141.4±13.9 UI/ml) and all except G*01:05N/G*01:05N (n = 9 129.5±16.8 UI/ml).

<p>Statistical comparison was based on Kruskal-Wallis one-way ANOVA followed by Dunn post-hoc test.</p

Comparison between A*02≈UTR-5≈G*01:03 (n = 24) and A*x≈UTR-5≈G*01:03 (n = 15, x corresponds to all HLA-A alleles except A*02).

<p>Statistical comparison was based on Mann-Whitney t-test (A*02≈UTR-5≈G*01:03 154.9±28.9 UI/ml; A*x≈UTR-5≈G*01:03 133.9±22.5 UI/ml). When outer points were excluded (212.4 UI/ml, 201.9 UI/ml, 92.2 UI/ml and 92.2 UI/ml for A*02≈UTR-5≈G*01:03; 86.4 UI/ml for A*x≈UTR-5≈G*01:03), the difference remained significant (p<0.05).</p

Comparison between UTR-2 (n = 103) and non-UTR-2 (n = 116) individuals related to sHLA-G production.

<p>Statistical comparison was based on Mann-Whitney t-test. (UTR-2 137.5±30.6 UI/ml vs. all except UTR-2 148.2±30.7 UI/ml). When six outer points were excluded (238.6 UI/ml, 217.6 UI/ml and 53.8 UI/ml for UTR-2; 242.2 UI/ml, 229.8 UI/ml and 226.5 UI/ml for non-UTR-2) UTR-2 individuals still displayed significantly lower values (p<0.05).</p

Comparison between two groups of women (3–25 years old n = 39 and over 26 years old n = 60) and men (3–25 years old n = 41 and over 26 years old n = 79) related to sHLA-G levels.

<p>Statistical comparison was based on Kruskal-Wallis one-way ANOVA followed by Dunn post-hoc test (girls 3–25 years old: 158.4±31.6 UI/ml; boys 3–25 years old: 152.0±31.6 UI/ml; women over 26 years old: 137.5±26.6 UI/ml; men over 26 years old: 135.5±26.4 UI/ml). NS: not significant; *: p>0.05; **: p<0.01; ***: p<0.001).</p

Description of the UTR haplotypes in the Malian population: nomenclature, polymorphism composition, estimated frequencies (Fq) with EM algorithm and association with coding HLA-G alleles.

<p>Comparison with previously published results on VBMD <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0082517#pone.0082517-DiCristofaro1" target="_blank">[41]</a>. Statistical differences between the two population frequencies were measured using chi-squared test (NS: no significant; *: p<0.05; **: p<0.01; ***: p<0.001).</p