249 research outputs found
Stem cells in dentistry – Part I: Stem cell sources
AbstractStem cells can self-renew and produce different cell types, thus providing new strategies to regenerate missing tissues and treat diseases. In the field of dentistry, adult mesenchymal stem/stromal cells (MSCs) have been identified in several oral and maxillofacial tissues, which suggests that the oral tissues are a rich source of stem cells, and oral stem and mucosal cells are expected to provide an ideal source for genetically reprogrammed cells such as induced pluripotent stem (iPS) cells. Furthermore, oral tissues are expected to be not only a source but also a therapeutic target for stem cells, as stem cell and tissue engineering therapies in dentistry continue to attract increasing clinical interest. Part I of this review outlines various types of intra- and extra-oral tissue-derived stem cells with regard to clinical availability and applications in dentistry. Additionally, appropriate sources of stem cells for regenerative dentistry are discussed with regard to differentiation capacity, accessibility and possible immunomodulatory properties
Recent advances in the understanding of cilia mechanisms and their applications as therapeutic targets
The primary cilium is a single immotile microtubule-based organelle that protrudes into the extracellular space. Malformations and dysfunctions of the cilia have been associated with various forms of syndromic and non-syndromic diseases, termed ciliopathies. The primary cilium is therefore gaining attention due to its potential as a therapeutic target. In this review, we examine ciliary receptors, ciliogenesis, and ciliary trafficking as possible therapeutic targets. We first discuss the mechanisms of selective distribution, signal transduction, and physiological roles of ciliary receptors. Next, pathways that regulate ciliogenesis, specifically the Aurora A kinase, mammalian target of rapamycin, and ubiquitin-proteasome pathways are examined as therapeutic targets to regulate ciliogenesis. Then, in the photoreceptors, the mechanism of ciliary trafficking which takes place at the transition zone involving the ciliary membrane proteins is reviewed. Finally, some of the current therapeutic advancements highlighting the role of large animal models of photoreceptor ciliopathy are discussed
Development of High Precision Timing Counter Based on Plastic Scintillator with SiPM Readout
High-time-resolution counters based on plastic scintillator with silicon
photomultiplier (SiPM) readout have been developed for applications to high
energy physics experiments for which relatively large-sized counters are
required. We have studied counter sizes up to mm^3 with
series connection of multiple SiPMs to increase the sensitive area and thus
achieve better time resolution. A readout scheme with analog shaping and
digital waveform analysis is optimized to achieve the highest time resolution.
The timing performance is measured using electrons from a Sr-90 radioactive
source, comparing different scintillators, counter dimensions, and types of
near-ultraviolet sensitive SiPMs. As a result, a resolution of ps at 1 MeV energy deposition is obtained for counter size mm^3 with three SiPMs ( mm^2 each) at each end of the
scintillator. The time resolution improves with the number of photons detected
by the SiPMs. The SiPMs from Hamamatsu Photonics give the best time resolution
because of their high photon detection efficiency in the near-ultraviolet
region. Further improvement is possible by increasing the number of SiPMs
attached to the scintillator.Comment: 11 pages, 17 figures, accepted by IEEE Trans. Nucl. Sc
Characterization of α-gustducin
Aims/Introduction: Taste receptors, T1rs and T2rs, and the taste‐selective G‐protein, α‐gustducin, are expressed outside the taste‐sensing system, such as enteroendocrine L cells. Here, we examined whether α‐gustducin also affects nutrition sensing and insulin secretion by pancreatic β‐cells.
Materials and Methods: The expression of α‐gustducin and taste receptors was evaluated in β‐cell lines, and in rat and mouse islets either by quantitative polymerase chain reaction or fluorescence immunostaining. The effects of α‐gustducin knockdown on insulin secretion and on cyclic adenosine monophosphate and intracellular Ca2+ levels in rat INS‐1 cells were estimated. Sucralose (taste receptor agonist)‐induced insulin secretion was investigated in INS‐1 cells with α‐gustducin suppression and in islets from mouse disease models.
Results: The expression of Tas1r3 and α‐gustducin was confirmed in β‐cell lines and pancreatic islets. Basal levels of cyclic adenosine monophosphate, intracellular calcium and insulin secretion were significantly enhanced with α‐gustducin knockdown in INS‐1 cells. The expression of α‐gustducin was decreased in high‐fat diet‐fed mice and in diabetic db/db mice. Sucralose‐induced insulin secretion was not attenuated in INS‐1 cells with α‐gustducin knockdown or in mouse islets with decreased expression of α‐gustducin.
Conclusions: α‐Gustducin is involved in the regulation of cyclic adenosine monophosphate, intracellular calcium levels and insulin secretion in pancreatic β‐cells in a manner independent of taste receptor signaling. α‐Gustducin might play a novel role in β‐cell physiology and the development of type 2 diabetes
Spectral and Timing Analysis of the accretion-powered pulsar 4U 1626-67 observed with Suzaku and NuSTAR
We present an analysis of the spectral shape and pulse profile of the
accretion-powered pulsar 4U 1626-67 observed with Suzaku and NuSTAR during a
spin-up state. The pulsar, which experienced a torque reversal to spin-up in
2008, has a spin period of 7.7 s. Comparing the phase-averaged spectra obtained
with Suzaku in 2010 and with NuSTAR in 2015, we find that the spectral shape
changed between the two observations: the 3-10 keV flux increased by 5% while
the 30-60 keV flux decreased significantly by 35%. Phase-averaged and
phase-resolved spectral analysis shows that the continuum spectrum observed by
NuSTAR is well described by an empirical NPEX continuum with an added broad
Gaussian emission component around the spectral peak at 20 keV. Taken together
with the observed Pdot value obtained from Fermi/GBM, we conclude that the
spectral change between the Suzaku and NuSTAR observations was likely caused by
an increase of the accretion rate. We also report the possible detection of
asymmetry in the profile of the fundamental cyclotron line. Furthermore, we
present a study of the energy-resolved pulse profiles using a new relativistic
ray tracing code, where we perform a simultaneous fit to the pulse profiles
assuming a two-column geometry with a mixed pencil- and fan-beam emission
pattern. The resulting pulse profile decompositions enable us to obtain
geometrical parameters of accretion columns (inclination, azimuthal and polar
angles) and a fiducial set of beam patterns. This information is important to
validate the theoretical predictions from radiation transfer in a strong
magnetic field.Comment: 19 pages, 14 figures, Accepted for publication in ApJ on May 5, 201
Japan and the European Union in the global economy. Bruegel Blueprint 22, 9 April 2014
This book is the final output of a strategic European Union-Japan research partnership, which we initiated in order to deepen our understanding of two major economies facing
similar challenges and opportunities. This publication brings together scholars from Kobe University and Bruegel along with selected outside contributors from Japan and
the EU from government and leading policy institutions
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