4 research outputs found
α-amanitin resistance in Drosophila melanogaster: A genome-wide association approach
© This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. We investigated the mechanisms of mushroom toxin resistance in the Drosophila Genetic Reference Panel (DGRP) fly lines, using genome-wide association studies (GWAS). While Drosophila melanogaster avoids mushrooms in nature, some lines are surprisingly resistant to α-amanitin-a toxin found solely in mushrooms. This resistance may represent a preadaptation, which might enable this species to invade the mushroom niche in the future. Although our previous microarray study had strongly suggested that pesticide-metabolizing detoxification genes confer α-amanitin resistance in a Taiwanese D. melanogaster line Ama-KTT, none of the traditional detoxification genes were among the top candidate genes resulting from the GWAS in the current study. Instead, we identified Megalin, Tequila, and widerborst as candidate genes underlying the α-amanitin resistance phenotype in the North American DGRP lines, all three of which are connected to the Target of Rapamycin (TOR) pathway. Both widerborst and Tequila are upstream regulators of TOR, and TOR is a key regulator of autophagy and Megalin-mediated endocytosis. We suggest that endocytosis and autophagy of α-amanitin, followed by lysosomal degradation of the toxin, is one of the mechanisms that confer α-amanitin resistance in the DGRP lines
Candidate genes resulting from the 180-line GWAS on 2.0 μg/g and the 37-line GWAS.
<p>Candidate genes resulting from the 180-line GWAS on 2.0 μg/g and the 37-line GWAS.</p
Manhattan plots for the three GWAS.
<p>A) 37-line GWAS using LC<sub>50</sub> values, B) 180-line GWAS on 2.0 μg/g α-amanitin, C) 180-line GWAS on 0.2 μg/g α-amanitin. Selected significant gene names are printed on the top right of the corresponding dots in the graphs.</p
Larval viability variation in the DGRP lines in response to α-amanitin.
<p>The y-axis shows individual viability values, while the x-axis represents the individual DGRP lines. The lines are sorted from lowest α-amanitin resistance (left) to highest α-amanitin resistance (right). The error bars represent the standard error of the mean (SEM). A) 180 lines tested on 0.2 μg/g α-amanitin. (Individual line numbers are not shown but can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0173162#pone.0173162.s001" target="_blank">S1 Table</a>). The y-axis represents the average number of flies hatched from 10 larvae placed on toxic food. B) 180 lines tested on 2.0 μg/g α-amanitin. (Individual line numbers are not shown but can be can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0173162#pone.0173162.s001" target="_blank">S1 Table</a>). The y-axis represents the average hatch counts out of 10 larvae placed on toxic food. C). The y-axis represents the LC<sub>50</sub> values of the 37-line subset. The line numbers are shown on the x-axis.</p