Additional file 1: Figure S1. of The first genome sequence of a metatherian herpesvirus: Macropodid herpesvirus 1

Relative transcript levels for the unique hypothetical MaHV-1 ORFs PW1 to PW6 at 4 h (grey bars) and 12 h (black bars) post infection (hpi) in wallaby fibroblast cells. Expression was normalised to the host housekeeping gene, GAPDH, and analysed by calculating mean normalised expression values. No viral transcripts were detected in uninfected cells. Error bars indicate standard deviation of three biological replicates. (TIF 8005 kb

Additional file 2: Table S1. of The first genome sequence of a metatherian herpesvirus: Macropodid herpesvirus 1

Oligonucleotides used for ORF transcription studies of the unique hypothetical MaHV-1 ORFs (PW1 to PW6) and the host housekeeping gene GAPDH. (DOC 37 kb

Additional file 1: Table S1. of Low genetic diversity among historical and contemporary clinical isolates of felid herpesvirus 1

FHV-1 sequencing metrics, viral genome sequence details. (DOCX 17 kb

Supplementary Fig. Relative transcript levels for the unique hypothetical MaHV-1 ORFs

<a href="https://static-content.springer.com/esm/art%3A10.1186%2Fs12864-016-2390-2/MediaObjects/12864_2016_2390_MOESM1_ESM.tif">Additional file 1: Figure S1.</a> Relative transcript levels for the unique hypothetical MaHV-1 ORFs PW1 to PW6 at 4 h (grey bars) and 12 h (black bars) post infection (hpi) in wallaby fibroblast cells. Expression was normalised to the host housekeeping gene, GAPDH, and analysed by calculating mean normalised expression values. No viral transcripts were detected in uninfected cells. Error bars indicate standard deviation of three biological replicates

Univariable analysis assessing select epidemiological variables as predictors for the presence of herpesvirus DNA in koalas ^a.

<p>^a Reference levels are indicated by odds ratio of 1.0. Results highlighted in bold (log likelihood p ≤ 0.25) represent variables included in the initial multivariable model, with the exception of season as the timing of sampling correlated with the location at which it occurred and was thus excluded. In the final model (n = 68) only the presence of <i>Chlamydia pecorum</i> was identified as a significant factor (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133807#pone.0133807.t008" target="_blank">Table 8</a>). n/a = not applicable.</p><p>Univariable analysis assessing select epidemiological variables as predictors for the presence of herpesvirus DNA in koalas <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133807#t005fn001" target="_blank">^a</a>.</p

Univariable analysis assessing select epidemiological variables as predictors for the presence of herpesvirus DNA in Tasmanian devils ^a.

<p>^a Reference levels are indicated by odds ratio of 1.0. Results highlighted in bold (log likelihood p ≤ 0.25) represent variables included in the initial multivariable model, with the exception of season as it was directly influenced by timing of management procedures, and therefore correlated with captive status and was thus excluded. In the final model (n = 50) only captivity was identified as a significant factor (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133807#pone.0133807.t008" target="_blank">Table 8</a>). n/a = not applicable.</p><p>Univariable analysis assessing select epidemiological variables as predictors for the presence of herpesvirus DNA in Tasmanian devils <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133807#t006fn001" target="_blank">^a</a>.</p

Electron micrographs of novel herpesviruses.

<p>Transmission electron microscopy was used to visualise herpesviruses in cultures of primary wombat kidney cells. Herpesvirus capsids (arrowheads) of VoHV-1 (A) and VoHV-2 (B) are shown. Bar = 100 nm.</p

Univariable analysis assessing select epidemiological variables as predictors for the presence of herpesvirus DNA in eastern grey kangaroos ^a.

<p>^a Reference levels are indicated by odds ratio of 1.0. Results highlighted in bold (log likelihood p ≤ 0.25) represent variables included in the initial multivariable model, with the exception of presence of pouch young/lactation as it is correlated with sex and thus excluded. Backward elimination of non-significant variables yielded no significant variables. Multivariable analysis was repeated including presence of pouch young/lactation as a variable instead of sex. Age was excluded from the model due to collinearity. In the final model (n = 42) only the absence of pouch young/lactation was identified as a significant factor (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133807#pone.0133807.t008" target="_blank">Table 8</a>). n/a = not applicable.</p><p>Univariable analysis assessing select epidemiological variables as predictors for the presence of herpesvirus DNA in eastern grey kangaroos <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133807#t004fn001" target="_blank">^a</a>.</p

Anatomical sites of herpesvirus DNA detection in swab samples collected from Australian marsupials in 2010 and 2011.

<p>^a Only species that were sampled in relatively large numbers, from multiple anatomical sites, are included.</p><p>^b Herpesvirus DNA was sometimes detected in more than one swab from the same animal, swabs were not collected from every anatomical site from every animal.</p><p>Anatomical sites of herpesvirus DNA detection in swab samples collected from Australian marsupials in 2010 and 2011.</p

Univariable analysis assessing select epidemiological variables as predictors for the presence of active herpesvirus infection in common wombats ^a.

<p>^a Reference levels are indicated by odds ratio of 1.0. Results highlighted in bold (log likelihood p ≤0.25) represent variables included in the initial multivariable model, with the exception of pouch young which was excluded due to zero prevalence of herpesvirus infection in females lactating/with pouch young. In the final model (n = 33) only age (adult/aged) and body condition score (≤ 2) were identified as significant factors (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133807#pone.0133807.t008" target="_blank">Table 8</a>). n/a = not applicable.</p><p>Univariable analysis assessing select epidemiological variables as predictors for the presence of active herpesvirus infection in common wombats <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133807#t007fn001" target="_blank">^a</a>.</p