Microbial Consortium Synergism for Promising Freshwater Culture Probiotic

Freshwater culture productivity can be improved by intensification. One of the most effective intensifications is the addition of probiotic agents. Bacillus subtilis, Lactobacillus lactis, and Saccharomyces cerevisiae have great potential as probiotic by improving water quality in freshwater culture. In the microbial consortium, the member of microbes has to have the ability to form synergism relationship to perform probiotic functions. Synergism interactions can be based on material transfer that relates to the energetic, cell to cell communication or physical protection. Advantage of microbial consortium synergism as a probiotic candidate has higher effectiveness and causes a broad spectrum effect than a single culture. The purpose of this study was to establish a synergism relationship between B. subtilis, L. lactis, and S. cerevisiaeas probiotic candidates in freshwater culture by testing using cross streak method and to know growth profile between themicrobes. The results showed that synergism occurred between all microbes depicting none inhibition zones between isolates. A half logarithmic phase at the growth curve can be known so microbial consortium probiotic formed