3,224 research outputs found
Evaluation of technical approaches to pronuclei injection
The transgenic technology makes it possible to introduce specific genetic alterations into the genome of all cells in an organism. This has opened entirely new possibilities to analyse the regulation and function of individual genes in a living animal. We have established a facility for the production of transgenic mice and have generated 37 different transgenic mouse strains. Here we analyse if the frequency of transgenic offspring correlates with the length of the introduced transgene and/or with the genetic background of the injected eggs. Our data suggest that the transgenic frequency is relatively independent of the length of the construct. The genetic background is more important and we find that specific steps in the process of generating transgenic mice are considerably more efficient in an F2 intercross between C57BL6 and CBA than in inbred C57BL6 mice. Finally we discuss how we have used the transgenic technology to analyse the regulation and function of genes inthe developing nervous system
Ambidexterity and Paradexterity: A typology of IT Governance contradictions
The theoretical construct of organizational ambidexterity addresses how organizations balance managerial contradictions such as exploitation and exploration or efficiency and flexibility. The underlying argument is that management should involve not a trade-off between two states, but simultaneous handling of contradictions. This paper expands the theory of organizational ambidexterity through introducing a typology of contradictions in the form of dichotomies and dualities within a particular management focus, i.e. IT Governance. The paper utilizes a previous study of IT Governance practice at two large, public universities to propose a typology and the concept of paradexterity. Through this, the paper seeks to add new knowledge to the fields of both organizational ambidexterity and IT Governance
Regeneration failure of Scots pine changes the species composition of young forests
How seedling mortality and browsing affects species composition of regenerating forests has been mostly studied on a small scale. Yet, large-scale analyses based on extensive data are essential for robust prediction of species composition in young forests. In this study, we used a dataset from a national inventory of young forests (1-4 metres in height) to investigate the species composition of young forests across Sweden. We found that most of the regenerated forest area (almost 90%) was planted with Norway spruce (southern Sweden) and Scots pine (northern Sweden). Regeneration of Norway spruce was generally relatively successful but as a consequence of seedling mortality and browsing, almost 40% of the area regenerated with Scots pine will probably not develop into pine-dominated stands. Thus, low survival of Scots pine seedlings and trees can profoundly change the trajectory of species composition of the young forest from what was originally intended, and a large proportion of the young stands may develop into mixtures of conifers and broadleaves. While such mixtures may benefit certain biodiversity and ecosystem services, a loss of Scots pine dominated stands may also have adverse impacts on the economic returns as well as pine-dependent biodiversity and recreational values
Using hybrid modelling to predict basal area and evaluate effects of climate change on growth of Norway spruce and Scots nine stands
When modelling forest growth, capturing the effects of climate change is needed for reliable longterm predictions and management choices. This remains a challenge because commonly used mensurational forest growth and yield models, relying on inventory data, cannot account for climate change effects. We developed hybrid physiological/mensurational basal area growth and yield models, which combine physiological response to climatic conditions and empirical relations. We included climate and site effects by replacing time with light sums of photosynthetically active radiation and modifying the latter with monthly soil water, vapour pressure deficit, temperature, and frost days. When parameterised with permanent sample plot data for Scots pine and Norway spruce across Sweden, the hybrid models could reproduce observations well, although with no increase in precision compared with time-based mensurational models. When considering different climate scenarios, a significant impact on productivity from climate change emerged. For example, a 2 degrees C warming enhanced Scots pine production by up to 14% in regions where temperatures were originally cooler and soil water deficit was low (i.e. northwest Sweden), but depressed it, up to 9%, elsewhere. Hence, climate-sensitive models that take local variations into account are necessary for accurate predictions and sustainable forest management
Early and repeated nutrient additions support far greater stemwood production in Norway spruce than traditional late-rotation fertilisation
Silvicultural techniques aimed at promoting forest biomass production can help meet the growing demand for renewable materials and mitigate climate change. One-time nitrogen (N) addition late in the rotation is a wellestablished method to stimulate growth in coniferous forests in northern Europe, but the potential gains from earlier and repeated fertiliser application remain uncertain. Here, we tested the impact of repeated fertilisation in juvenile Norway spruce stands across 9 sites covering a wide range of growing conditions over a 700 km stretch from central to southern Sweden. We tested the fertilisation effects using two separate studies: i) an interval trial with a fertilisation frequency of one (F1), two (F2), or three years (F3) performed at plot-level across five sites (2002-2014), and ii) a practice-oriented trial with a two-year fertilisation interval (F2) applied at stand-level and replicated at four sites (2003-2013). The composition of the nutrient mix in each plot was optimised based on foliar nutrient analyses. In the interval trial, all three fertilisation schedules strongly increased periodic annual increment (PAI) (F1: 105 %, F2: 93 %, F3: 79 %) relative to the unfertilised control, resulting in more than a doubling of stem volume yield in the F1 and F2 treatments (110 % and 120 %, respectively) and a significantly smaller but still sizeable yield stimulation of 82 % in the F3 treatment. Nitrogen use efficiency (NUE, stemwood volume increase per unit mass of N added) was similar among fertilisation intervals (on average 130 m3 ha-1 1000 kg N-1), indicating that the extra N provided through yearly fertilisation (F1) is redundant given the similar stemwood yields in the F2 treatment. In the practice-oriented trial, the sole F2 treatment increased PAI by 95 % over the control, translating into a yield stimulation of 114 % and an almost identical NUE to that of the interval trial. NUE greatly exceeded the figures typically observed with traditional late-rotation fertilisation and correlated inversely with baseline site productivity (using site index as a proxy) in the F1 and F2 treatments (the latter pooled across the two trials). Our results clearly indicate that nutrient limitation restricts growth and carbon (C) capture in young Norway spruce plantations in northern Europe to less than half of their potential, highlighting repeated fertilisation at nutrient-poor sites as an effective management tool to support a growing bioeconomy and enhance C sequestration
Abandoning conversion from even-aged to uneven-aged forest stands - the effects on production and economic returns
There is growing interest in continuous cover forestry in Sweden. The historical domination of even-aged forest management means there is a need to focus on methods for transforming even-aged to uneven-aged stands. Practical conversion management today is highly adaptive, and the possibility of failures, such as persistent lack of tree recruitment, must be allowed for. We used simulations to evaluate various scenarios in which conversion management is introduced at different development stages in even-aged stands and then abandoned in favour of clear-felling. A shift towards an inverse J-shaped diameter distribution at the end of the simulations was most evident in northern Sweden and with an early introduction of conversion forestry. The largest losses were incurred in a scenario where the conversion management started with removing larger trees at pre-commercial thinning: it reduced the volume of production by up to 25% and, if clear-felled, resulted in a lower land expectation value than conventional management. Earlier decision to abandon conversion management reduced the losses, but the effect of timing was minor. In summary, the results indicate that conversion management could be started and abandoned without any major economic loss during the timeframe of a normal rotation
Modelling potential yield capacity in conifers using Swedish long-term experiments
Information on forest site productivity is a key component to assess the carbon sequestration potential of boreal forests. While site index (SI) is commonly used to indicate forest site productivity, expressions of SI in the form of yield capacity (potential maximum mean annual volume increment) is desirable since volume yield is central to the economic and ecological analyses of a given species and site. This paper assessed the functional relationship between SI and yield capacity on the basis of yield plot data from long-term experiments measured over several decades for Norway spruce (Picea abies), Scots pine (Pinus sylvestris), Lodgepole pine (Pinus contorta) and Larch (Larix decidua and Larix sibirica) in Sweden. Component models of total basal area and volume yield were also developed. SI was determined by existing height development functions using top height and age, whereas functions for stand-level (m2 ha- 1) basal area development were constructed based on age, SI and initial stand density using difference equations and nonlinear mixed-effects models. The relation between volume yield (m3 ha- 1) and top height was adjusted with total basal area production through nonlinear mixed-effects models. Species-specific parametric regression models were used to construct functional relationships between SI and yield capacity. The root mean square errors of the species-specific models ranged from 2 to 6% and 10-18% of the average values for the basal area and volume equations, respectively. For the yield capacity functions, the explained variations (R2) were within 80-96%. We compared our yield capacity functions to earlier functions of the species and significant differences were observed in both lower and higher SI classes, especially, for Scots pine and Norway spruce. The new functions give better prediction of yield capacity in current growing conditions; hence, they could later be used for comparing tree species' production under similar site and management regimes in Sweden
Will intensity of forest regeneration measures improve volume production and economy?
The prevailing regeneration methods in Scandinavian countries are artificial regeneration methods including measures such as site preparation and planting. These measures are considered to be a part of a more intensive forest management and require an initial investment. The use of artificial regeneration measures can, however, increase the growth of a forest stand. In this study, the purpose was to investigate if such an investment is profitable by comparing three different intensity levels (low, medium and high) applied during the regeneration phase, with aspect on both economics (LEV, land expectation value) and growth (MAI, mean annual increment) after a full rotation. The forest stands used in this study were regenerated between 1984 and 1988 and the future growth of the stands was simulated using Heureka StandWise. It was clear that naturally regenerated (low intensity) stands resulted in better economics than stands actively regenerated (medium and high intensity). However, actively regenerated stands resulted in both higher volume production and growth, and the uncertainty of regeneration success was reduced using artificial regeneration measures. These factors are important when considering both the ongoing mitigation of carbon dioxide in the atmosphere and future access to raw material
Pre-Commercial Thinning Increases the Profitability of Norway Spruce Monoculture and Supports Norway Spruce-Birch Mixture over Full Rotations
Pre-commercial thinning (PCT) is a common measure in Norway spruce (Picea abies L. Karst.) stands but the profitability of doing PCT and timing of PCT has not been fully investigated over a full rotation. Further, limited knowledge is available for mixed forest management compared to monocultures. In this study, different PCT strategies were tested to investigate the effect of PCT and timing of PCT on the production and profitability of Norway spruce monocultures and mixed Norway spruce-birch stands. A forest decision support system was used to simulate stand development during the whole rotation. Our study findings show that there is a positive effect of PCT on Norway spruce plantations' long-term profitability but that the timing of PCT has little effect on profitability. However, site variation might influence the effect of PCT timing on the profitability of Norway spruce stands. Moreover, we also found that retaining 1000 Norway spruce ha(-1) and 1000 birch ha(-1) after PCT supports a mixture of Norway spruce and birch over a full rotation, with little or no economic loss compared to pure Norway spruce stands. Therefore, such a mixture can lead to profitable production while still providing other ecosystem services
Modelling effects of regeneration method on the growth and profitability of Scots pine stands
Despite numerous studies there are still uncertainties regarding regeneration strategies that are optimal for productivity and profitability. Thus the aim of this study was to establish effects of three regeneration methods (planting, direct seeding and natural regeneration) on the production and profitability of Scots pine (Pinus sylvestris L.) stands in southern Sweden. Long-term stand development was simulated, with the StandWise application of the Heureka decision support system, starting from short-term regeneration outcomes observed in several field experiments at sites with relatively high productivity (H100 site indices, i.e. heights of dominant pines at 100 years: 27-30 m). Financial and production results of each approach were assessed in terms of Land Expectation Value (LEV) and Mean Annual Increment (MAI), respectively, across a whole rotation. Planting on clear-cuts with 1600-3265 seedlings per hectare resulted in the highest profitability and production, whereas high-density planting (10,000 seedlings per hectare) resulted in negative LEV. However, sensitivity analysis showed that the results depended on the interest rate. Retention of seed-trees incurred additional costs relative to single-operation clear felling. In contrast, retention of shelter-trees had good financial results (at 0% and 2.5% interest rate), although they depended on the site index and average tree size
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