5 research outputs found
Changes of sFas and sFasL, oxidative stress markers in serum and follicular fluid of patients undergoing IVF
The Fas-Fas Ligand interaction is one of the essential events for the
induction of apoptosis whereas the exact role of their soluble forms in
the reproductive system is still not fully understood. Also oxidative
stress in the pathogenesis of infertility causing diseases in women and
has been suggested as one of the important factors that negatively
affect IVF outcome. In this study, our aim was to evaluate serum and
follicular fluid levels of soluble Fas soluble Fas Ligand,
malondialdehyde, superoxide dismutase and total antioxidant capacity in
patients undergoing IVF and compared with controls.
This study included 109 patients. Patients were classified as
unexplained infertility (N = 31), PCOS (N = 19), tubal factor (N = 9)
and endometriosis (N = 10) and compared with male factor infertility (N
= 40) that was the control group. sFas and sFasL levels were measured by
immunoassay method. MDA, SOD and TAC levels were measured by
colorimetric method.
Patients with unexplained infertility, PCOS and tubal factor had
significantly lower sFas levels compared with their controls
(respectively, p < 0.01, p < 0.05, p < 0.05). However, SOD activity in
unexplained infertility, PCOS and endometriosisgroupswere significantly
higher than control group (p < 0.01).Decreased follicular fluid TAC
levels were found in all patient groups compared with controls
(respectively, p < 0.01, p < 0.05, p < 0.01, p < 0.01).Patients with
tubal factor had significantly higher serum sFasL (p < 0.05), but lower
follicular fluid sFasL levels (p < 0.05) compared with unexplained
infertility. Tubal factor and endometriosis groups had lowerfollicular
fluid TAC levels compared to unexplained infertility and PCOSgroups (p <
0.01).
In this study, serum and follicular fluid sFas levels were decreased and
antioxidant activity was impaired in infertility, possibly implying
increased apoptosis. Especially in unexplained infertility group changes
in this parametres more remarkable