68 research outputs found

    Composite Steel-Concrete Beam Analysis using Different Methods

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    The paper compares the following methods for analysis of composite steel-concrete beams: the “exact” analysis method, simplified method, effective modulus method and the method proposed by Eurocode 4 design code. The exact analysis method is based on the application of linear integral operators. Simplified analysis is derived from the exact method and adopts the assumption that generalized displacements change linearly with the concrete creep function. Effective modulus method and the method proposed by the Eurocode 4 design code are algebraic methods, widely used in practice. The results obtained using the mentioned four methods are compared on one example

    Synthesis, structural characterization and antimicrobial activity of silver(I) complexes with 1-benzyl-1H-tetrazoles

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    Herein, we report the synthesis and structural characteristics of three tetrazole-containing compounds, 1-benzyl-1H-tetrazole (bntz), 1-benzyl-1H-tetrazol-5-amine (bntza) and 1-(4-methoxybenzyl)-1H-tetrazol-5-amine (mbntza) and the corresponding silver(I) complexes of the general formula [Ag(NO3-O)(L-N4)(2)](n), L = bntz (1), bntza (2) and mbntza (3). Silver(I) complexes 1-3 and 1-benzyl-1H-tetrazoles have been studied in detail by NMR, IR and UV-Vis spectroscopic methods and the structures of 1 and 2 have been determined by single-crystal X-ray diffraction analysis. The results of these analyses revealed a monodentate coordination of the ligands to Ag(I) ion via the N4 tetrazole nitrogen. The antimicrobial potential of silver(I) complexes 1-3 was evaluated against the broad panel of Gram-positive and Gram-negative bacteria and fungi, displaying their remarkable inhibiting activity with MIC (minimal inhibitory concentration) values in the range 2-8 and 0.16-1.25 mu g/mL (3.8-16.3 and 0.31-2.15 mu M), respectively. On the other hand, 1-benzyl-1H-tetrazoles used for the synthesis of the silver(I) complexes were not active against the investigated strains, suggesting that the activity of the complexes originates from the Ag(I) ion exclusively. Moreover, silver(I) complexes 1-3 have good therapeutic potential, which can be deduced from their moderate cytotoxicity on the human fibroblast cell line MRC5, with IC50 values falling in the range 30-60 mu g/mL (57.7-103.4 mu M).Related to published version: [https://imagine.imgge.bg.ac.rs/handle/123456789/1144]This is the peer-reviewed version of the article: Andrejević, T. P., Nikolić, A. M., Glišić, B., Wadepohl, H., Vojnović, S., Zlatović, M., Petković, M., Nikodinović-Runić, J., Opsenica, I. M., & Djuran, M. (2018). Synthesis, structural characterization and antimicrobial activity of silver(I) complexes with 1-benzyl-1H-tetrazoles. Polyhedron, 154, 325–333. [https://doi.org/10.1016/j.poly.2018.08.001

    Utjecaj ekspozicije krošnje na morfološka svojstva iglica devet četinjača

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    The aim of this research was to investigate if the crown exposure of some conifers influenced to needle properties. The leaf morphological traits of sixty-six trees of nine conifers: Atlas cedar, Austrian pine, Blue spruce, Douglas fir, European spruce, European yew, Serbian spruce, Silver fir, and White fir, from six Belgrade parks, were analyzed. Five needles were measured from each of the four main crown exposures. Length, width, area and perimeter of needles were investigated. Species, parks in which they were found, as well as crown exposures, differed mostly in needle length and needle width. Correlations between measured needle traits were determined by linear regression analysis. Strong positive correlations were found between the length, perimeter, and area of needles. The differences among the species in terms of light requirement determine species for individual planting as light-loving or partial shade species (Atlas cedar, European spruce, Serbian spruce, Blue spruce, Austrian pine and Douglas fir), or for group planting as shade-loving species (Silver fir, White fir and European yew).Analizirano je 66 stabala devet vrsta četinjača: atlaskog cedra, crnog bora, bodljikave smreke, duglazije, obične smreke, šumske tise, Pančićeve omorike, koloradske  jele i obične jele, iz šest beogradskih parkova. Analizirano je pet iglica sa svake od četiri glavne ekspozicije krošnje. Ispitivana je duljina, širina, površina i opseg iglica. Vrste, parkovi u kojima su pronađene, kao i ekspozicije njihovih kruna, razlikovali su se po duljini i širini iglica. Korelacije između izmjerenih svojstava iglica određene su linearnom regresijskom analizom. Utvrđene su jake pozitivne korelacije između duljine, opsega i površine iglica. Razlike među vrstama u zahtjevima za svjetlom određuju vrste za pojedinačnu sadnju kao vrste koje vole svjetlo ili polusjenu (atlaski cedar, obična smreka, Pančićeva omorika, bodljikava smreka, crni bor i duglazija) ili za grupnu sadnju kao sjenoljubne vrste (obična jela, dugoigličava jela i šumska tisa)

    Projekat BEWARE - približavanje otvorenim podacima o klizištima

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    Project on “Harmonization of landslide data and training of municipalities for its monitoring (BEyond landslide aWAREness) BEWARE“, was implemented by the Geological Survey of Serbia, and the University of Belgrade Faculty of Mining and Geology during 2015-2016. The Project partners were UNDP Office in Serbia, Ministry of Mining and Energy and Agency for Public Investments of the Republic of Serbia. The overall aim of BEWARE project was to standardize post-event landslide database and closely involve local community of 27 municipalities affected by May 2014 flooding and landslides episode in Serbia (Figure 1a, 1c), and prepare them to cope with catastrophic events in the future. In this paper, we are presenting main BEWARE project activities and results implemented in local communities of Serbia after the May 2014 event

    Terpenes as Useful Markers in Differentiation of Natural Populations of Relict Pines Pinus heldreichii , P . nigra , and P . peuce

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    Comparative analysis of terpene diversity and differentiation of relict pines Pinus heldreichii, P. nigra, and P. peuce from the central Balkans was performed at the population level. Multivariate statistical analyses showed that the composition of needle terpenes reflects clear divergence among the pine species from different subgenera: P. peuce (subgenus Strobus) vs. P. nigra and P. heldreichii (subgenus Pinus). In addition, despite the described morphological similarities and the fact that P. nigra and P. heldreichii may spontaneously hybridize, our results indicated differentiation of their populations naturally growing in the same area. In accordance with recently proposed concept of 'flavonic evolution' in the genus Pinus, we assumed that the terpene profile of soft pine P. peuce, defined by high amounts of six monoterpenes, is more basal than those of hard pines P. nigra and P. heldreichii, which were characterized by high content levels of mainly sesquiterpenes. In order to establish precise positions of P. heldreichii, P. nigra and P. peuce within the taxonomic and phylogenetic tree, as well as develop suitable conservation strategies and future breeding efforts, it is necessary to perform additional morphological, biochemical, and genetic studies

    Distinct cytotoxic mechanisms of pristine versus hydroxylated fullerene

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    The mechanisms underlying the cytotoxic action of pure fullerene suspension (nano-C-60) and water-soluble polyhydroxylated fullerene [C-60(OH)(n)] were investigated. Crystal violet assay for cell viability demonstrated that nano-C-60 was at least three orders of magnitude more toxic than C-60(OH)(n) to mouse L929 fibrosarcoma, rat C6 glioma, and U251 human glioma cell lines. Flow cytometry analysis of cells stained with propidium iodide (PI), PI/annexin V-fluorescein isothiocyanate, or the redox-sensitive dye dihydrorhodamine revealed that nano-C-60 caused rapid (observable after few hours), reactive oxygen species (ROS)-associated necrosis characterized by cell membrane damage without DNA fragmentation. In contrast, C-60(OH)(n) caused delayed, ROS-independent cell death with characteristics of apoptosis, including DNA fragmentation and loss of cell membrane asymmetry in the absence of increased permeability. Accordingly, the antioxidant N-acetylcysteine protected the cell lines from nano-C-60 toxicity, but not C-60(OH)(n) toxicity, while the pan-caspase inhibitor z-VAD-fmk blocked C-60(OH)(n)-induced apoptosis, but not nano-C-60-mediated necrosis. Finally, C-60(OH)(n) antagonized, while nano-C-60 synergized with, the cytotoxic action of oxidative stress-inducing agents hydrogen peroxide and peroxynitrite donor 3-morpholinosydnonimine. Therefore, unlike polyhydroxylated C-60 that exerts mainly antioxidant/cytoprotective and only mild ROS-independent pro-apoptotic activity, pure crystalline C-60 seems to be endowed with strong pro-oxidant capacity responsible for the rapid necrotic cell death

    Application of Landsat-derived NDVI in monitoring and assessment of vegetation cover changes in Central Serbia

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    This paper evaluates the application of the Normalised Difference Vegetation Index (NDVI) in the monitoring and assessment of temporal vegetation cover changes (from 2006 to 2014) in three municipalities of Central Serbia: Topola, Jagodina and Kuršumlija. Additionally, special focus is placed on the analysis of the forest areas and the possible use of NDVI in the forest management sector. Results of the NDVI applied through Idrisi software identify all vegetation cover types and their typical values for presented case studies and observed periods. Obtained results for Serbian case studies indicate two major observations outlined for the investigated period. It was noticed that vegetation cover is experiencing a certain decrease, and that certain discrepancies exists between the NDVI and official forest area statistics for certain municipalities. The study outlines the positive outcomes of the applied remote sensing techniques, especially for southern Serbian municipalities where illegal logging activities are pronounced. Hence, this method proved very promising for countries performing national forest inventories, such as Serbia, providing local forest managers with several essential up-to-date information about vegetation cover changes on an annual basis. © 2019 Carpathian Journal of Earth and Environmental Sciences

    Common variable immunodeficiency complicated with hemolytic uremic syndrome

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    Common variable immunodeficiency is a primary immunodeficiency disease characterized by reduced serum immunoglobulins and heterogeneous clinical features. Recurrent pyogenic infections of upper and lower respiratory tracts are the main clinical manifestations of common variable immunodeficiency. Hemolytic uremic syndrome is a multisystemic disorder characterized by thrombocytopenia, microangiopathic hemolytic anemia, and organ ischemia due to platelet aggregation in the arterial microvasculature. This is one of the rare cases of patients diagnosed with common variable immunodeficiency, which was complicated by hemolytic uremic syndrome

    Morpho-anatomical characteristics and content of nutritive macro elements in needles of fir and spruce and their varieties in Serbia

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    The aim of the study is to establish, based on the morphological characteristics of shoots and morpho-anatomical characteristics of needles as well as the content of micro-elements in needles, whether there are differences between Pyramidal Fir (Abies alba var. pyramidalis Carr.) and European Silver Fir (Abies alba Mill.), and between Mišić’s spruce (Picea abies var. misicii Matović & Pavlović) and Common Spruce (Picea abies (L.) Karst.), and to determine in which characteristics these differences are most pronounced. This study showed that Pyramidal fir (PF) has a two-times higher number of lateral shoots in comparison to European silver fir (ESF), but also shorter and thinner shoots and a lower number of needles, which are slightly less densely distributed on a shoot. In addition, PF terminal shoots are significantly longer and thicker than the lateral, and have a higher number of needles. Two-year-old PF needles are longer, wider and thicker in comparison to ESF needles, and have higher values with respect to diameter of the central cylinder, thickness of cuticle and epidermis, thickness of hypodermis and diameter of resin canals. The most pronounced differences between PF and ESF are related to length of shoots, needle width and diameter of the central cylinder (statistically significant differences). In comparison to ESF, PF has a higher percentage of nitrogen, phosphorus and potassium in needles; however, this is statistically significant only in case of phosphorus. The most apparent morphological specificity of Mišić’s spruce (MS) is a rare occurrence of lateral shoots (only once in several years), unlike Common spruce (CS), which has 2-3 new shoots every year. MS has far longer and thicker shoots, while its needles are more numerous, longer, thinner and more densely distributed on the shoot in comparison to CS. Additionally, in comparison to CS, MS needles have a larger diameter of central cylinder, but lower thickness of cuticle with epidermis, lower thickness of hypodermis and smaller diameter of the resin canals. The most pronounced differences between MS and CS are related to length, width and thickness of needles and diameter of central cylinder (statistically significant differences). In comparison to CS, MS has a higher percentage of nitrogen, phosphorus and potassium in needles; however, this is not statistically significant
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