Fluorescence-based monitoring of ribosome assembly landscapes

Background Ribosomes and functional complexes of them have been analyzed at the atomic level. Far less is known about the dynamic assembly and degradation events that define the half-life of ribosomes and guarantee their quality control. Results We developed a system that allows visualization of intact ribosomal subunits and assembly intermediates (i.e. assembly landscapes) by convenient fluorescence-based analysis. To this end, we labeled the early assembly ribosomal proteins L1 and S15 with the fluorescent proteins mAzami green and mCherry, respectively, using chromosomal gene insertion. The reporter strain harbors fluorescently labeled ribosomal subunits that operate wild type-like, as shown by biochemical and growth assays. Using genetic and chemical perturbations by depleting genes encoding the ribosomal proteins L3 and S17, respectively, or using ribosome-targeting antibiotics, we provoked ribosomal subunit assembly defects. These defects were readily identified by fluorometric analysis after sucrose density centrifugation in unprecedented resolution. Conclusion This strategy is useful to monitor and characterize subunit specific assembly defects caused by ribosome-targeting drugs that are currently used and to characterize new molecules that affect ribosome assembly and thereby constitute new classes of antibacterial agents

Effect of cold rolling on consistent pattern and mechanisms deformation, structural and phase transitions and mechanical properties of single crystals steel Fe-28Mn-2.6Al-1.3C

The dependence of microhardness of Fe-28Mn-2.6Al-1.3C single crystals on the degree of precipitation and deformation temperature is established. It can be divided into three sections: in sections I and III, the microhardness is slightly dependent on the orientation of the sample, while in section II the orientation dependence of microhardness is manifested. The orientation dependence of the microhardness is determined by the orientation dependence of the dislocation structure and the deformation mechanism (slip and / or twinning). Rolling does not lead to the realization of deformation phase transitions of steel Fe-28Mn-2.6Al-1.3C

Система управления персоналом в рудоуправлении "Кальмакир" АО "Алмалыкский ГМК"

Целью написаний данной выпускной квалификационной работы является исследование системы управления персоналом в рудоуправлении Кальмакир АО "АГМК".Цель исследования реализуется в решении следующих задач: -раскрыть основные понятия, характеристику и структуру системы управления персоналом предприятия; -исследовать элементы и методы управления персоналом; -исследовать отечественный и зарубежный опыт управления персоналом; -дать экономическую характеристику деятельности рудоуправления Кальмакир АО "АГМК"; -проанализировать систему управления персоналом рудоуправления Кальмакир АО "АГМК"; -разработать рекомендации по совершенствованию управления персоналом рудоуправления Кальмакир АО "АГМК"The purpose of the writing of this final qualifying work is to study the personnel management system in the mine Kalmakir JSC "AGMK".The purpose of the study is implemented in solving the following tasks: - to reveal the basic concepts, characteristics and structure of the personnel management system of the enterprise; -to study the elements and methods of personnel management; -to study the domestic and foreign experience of personnel management; - to give an economic description of the activities of the mine management Kalmakir JSC "AGMK"; - to analyze the personnel management system of the mine management Kalmakir JSC " AGMK"; -to develop recommendations to improve personnel management of the mine group Kallmaker JSC "Amursk metallurgical plant

Design of the HTS Fusion Conductors for TF and CS Coils

The main electrical and mechanical requirements for the LTS fusion conductors of DEMO are retained as a starting point for the development of HTS fusion cables. Based on the HTS coated conductor technology, a flat cable design was proposed by CRPP Swiss Plasma Center (SPC) using the strands made of twisted stack of tapes soldered into copper profiles. Analytical modeling of the cable geometry is developed and presented in this work. The model was used to estimate various properties of cable. Addressing the issue of bending strain and related performance degradation, an optimization model of the cable properties was built, which allows to best fulfill the cable requirements. Design options are developed for both toroidal field (TF) coils operating at 63 kA and central solenoid (CS) coils operating at 50 kA. Paying attention to the DC and pulsed operation of the TF and CS coils, proposals for the design of the forced-flow HTS conductors are reported and discussed for each type of the coils

Design Optimization of Round Strands Made by Twisted Stacks of HTS Tapes

Atwisted stack of high-temperature superconducting (HTS) tapes soldered into copper profiles was used as a strand in the fabrication of the 60-kA/ 12-T HTS cable prototype at the Center for Research in Plasma Physics (CRPP). Considering the strand in general as a modular element for the high-current cables, design parameters of the strand need to be optimized in order to best fulfill requirements of appropriate application field. During the development program of the HTS fusion cable at CRPP, influence of the design parameters on the strand's performance was obtained as input data for the optimization process. In this paper, we summarize the results of the twisting, bending, and transverse pressure tests on strands of various tapes and profile's geometries. Finite-element model for the transverse pressure test was developed, validated with the test results, and used for the design proposals. Effect of the stack aspect ratio, tape properties, and anisotropy, as well as selection of HTS material for the strand, will be discussed

Potsdam Library of Astronomical Photographic Plates

ACM Computing Classification System (1998): J.2.We present the Potsdam photographic plate library at the Leibniz Institute for Astrophysics Potsdam. It includes plate archives, data from plate index catalogues and extracts from astronomical logbooks, as well as digitized plate images and interfaces for accessing all the available information. The plate index catalogues and the digitized images of the Potsdam astronomical photographic plates are prepared according to the standards of the Wide-Field Plate Database and German Astrophysical Virtual Observatory. The high-resolution digitization of the plates (in standardized FITS file format), the low-resolution plate previews (in TIFF and JPEG file format), as well as suitable digitization of the catalogues, logbooks, and relevant scientific research papers (in JPEG and TIFF) are made using commercial flatbed scanners.This work is supported by the bilateral project STE 710/6-1, 2009-11-20, between BAS/DFG, and partially by BG NSF DO-02-273 and DO-02-275 grants

Multifaceted Stoichiometry Control of Bacterial Operons Revealed by Deep Proteome Quantification

More than half of the protein-coding genes in bacteria are organized in polycistronic operons composed of two or more genes. It remains under debate whether the operon organization maintains the stoichiometric expression of the genes within an operon. In this study, we performed a label-free data-independent acquisition hyper reaction monitoring mass-spectrometry (HRM-MS) experiment to quantify the Escherichia coli proteome in exponential phase and quantified 93.6% of the cytosolic proteins, covering 67.9% and 56.0% of the translating polycistronic operons in BW25113 and MG1655 strains, respectively. We found that the translational regulation contributes largely to the proteome complexity: the shorter operons tend to be more tightly controlled for stoichiometry than longer operons; the operons which mainly code for complexes is more tightly controlled for stoichiometry than the operons which mainly code for metabolic pathways. The gene interval (distance between adjacent genes in one operon) may serve as a regulatory factor for stoichiometry. The catalytic efficiency might be a driving force for differential expression of enzymes encoded in one operon. These results illustrated the multifaceted nature of the operon regulation: the operon unified transcriptional level and gene-specific translational level. This multi-level regulation benefits the host by optimizing the efficiency of the productivity of metabolic pathways and maintenance of different types of protein complexes

System size and centrality dependence of the balance function in A+A collisions at sqrt[sNN]=17.2 GeV

Electric charge correlations were studied for p+p, C+C, Si+Si, and centrality selected Pb+Pb collisions at sqrt[sNN]=17.2 GeV with the NA49 large acceptance detector at the CERN SPS. In particular, long-range pseudorapidity correlations of oppositely charged particles were measured using the balance function method. The width of the balance function decreases with increasing system size and centrality of the reactions. This decrease could be related to an increasing delay of hadronization in central Pb+Pb collisions

System size and centrality dependence of the balance function in A + A collisions at sqrt s NN = 17.2 GeV

Electric charge correlations were studied for p+p, C+C, Si+Si and centrality selected Pb+Pb collisions at sqrt s_NN = 17.2$ GeV with the NA49 large acceptance detector at the CERN-SPS. In particular, long range pseudo-rapidity correlations of oppositely charged particles were measured using the Balance Function method. The width of the Balance Function decreases with increasing system size and centrality of the reactions. This decrease could be related to an increasing delay of hadronization in central Pb+Pb collisions