Expression analysis of Notch signaling pathway between normal and PE placentas.

<p><b>P-value (c):</b> crude p-value; <b>P-value (a):</b> P-value adjusted for gestation period, mode of delivery and smoking during pregnancy. All values are presented as mean ± SEM.</p><p>Expression analysis of Notch signaling pathway between normal and PE placentas.</p

Primer sequences, annealing temperatures and amplicon sizes for the qRT-PCR analysis of the studied genes.

<p>Primer sequences, annealing temperatures and amplicon sizes for the qRT-PCR analysis of the studied genes.</p

Notch signaling pathway pair-wise mRNA co-expression analysis in normal placentas.

<p><b>CC</b>: Correlation coefficient.</p><p>Notch signaling pathway pair-wise mRNA co-expression analysis in normal placentas.</p

Clinical characteristics of the study groups.

<p>^a Student’s T test</p><p>^b Mann-Whitney U test</p><p>^c Chi-square test</p><p>^d Fisher’s exact test</p><p>All tests were 2-tailed.</p><p>Clinical characteristics of the study groups.</p

Notch signaling pathway pair-wise mRNA co-expression analysis in preeclamptic placentas.

<p><b>CC</b>: Correlation coefficient.</p><p>Notch signaling pathway pair-wise mRNA co-expression analysis in preeclamptic placentas.</p

Schematic representation of Notch pathway molecules mRNA expression profile in our series of preeclampsia-complicated late preterm and term placentas.

<p>Red: Downregulation; White: Normal expression; Sky Blue: Overexpression.</p

Schematic depiction of the studied Notch signaling pathway molecules in late preterm and term placentas.

<p>Schematic depiction of the studied Notch signaling pathway molecules in late preterm and term placentas.</p

Box and whisker plots depicting statistically significant associations in preeclampsia-complicated late preterm and term placentas.

<p>(A) <i>NOTCH3</i> was not expressed in preeclamptic (PE) women that smoked during their pregnancy versus women with PE that did not smoke (0.00±0.00 vs. 0.29±0.09, p = 0.029). (B) <i>DLL3</i> mRNA expression was higher in babies born from PE pregnancies with Birth Weight Centile (BWC) <5 compared with babies born from PE pregnancies with BWC >5 (0.91±0.30 vs. 0.42±0.19, p = 0.041). (C) <i>HEY2</i> transcript levels were increased in women with PE who were on their fist parity versus women with PE that gave birth to their 2^nd or 3^rd child (0.44±0.13 vs. 0.05±0.02, p = 0.034). (D) <i>HEY2</i> mRNA expression was higher in women with PE who gave birth with a Caesarian Section compared with women with PE who gave birth naturally (0.63±0.17 vs. 0.15±0.05, p = 0.028). (E) NOTCH3 intracellular domain (NICD3) protein levels were higher in babies born from women with pregnancies complicated by PE with BWC <5 versus babies born from women with pregnancies complicated by PE with BWC >5 (1.15±0.24 vs. 0.16±0.07, p = 0.028). The thick line near the center of each rectangular box represents the median value, the bottom and top edges of the box indicate the 1^st (Q₁) and 3^rd (Q₃) quartiles, and the ends of the whiskers depict the 10^th (P₁₀) and 90^th (P₉₀) percentiles.</p