4 research outputs found
Lysosomal Alterations in Peripheral Blood Mononuclear Cells of Parkinson's Disease Patients
Background: Reduced expression of lysosomal-associated membrane protein
2a and heatshock-cognate 70 proteins, involved in chaperone-mediated
autophagy and of glucocerebrosidase, is reported in PD brains. The aim
of this study was to identify systemic alterations in
lysosomal-associated membrane protein 2a, heatshock cognate-70, and
glucocerebrosidase levels/activity in peripheral blood mononuclear cells
from PD patients.
Methods: Protein/mRNA levels were assessed in PD patients from
genetically undetermined background, alpha-synuclein (G209A/A53T), or
glucocerebrosidase mutation carriers and age-/sex-matched controls.
Results: Heatshock cognate 70 protein levels were reduced in all PD
groups, whereas its mRNA levels were decreased only in the genetically
undetermined group. Glucocerebrosidase protein levels were decreased
only in the genetic PD groups, whereas increased mRNA levels and
decreased activity were detected only in the glucocerebrosidase mutation
group.
Conclusions: Reduced heatshock cognate-70 levels are suggestive of an
apparent systemic chaperone-mediated autophagy dysfunction irrespective
of genetic background. Glucocerebrosidase activity may serve as a
screening tool to identify glucocerebrosidase mutation carriers with PD.
(C) 2015 International Parkinson and Movement Disorder Societ