Proliferative lesions of the breast lobules. A morphometric analysis.

A morphometric study was carried out on proliferative lesions of the breast lobules, including cases of atypical lobular hyperplasia (ALH), lobular carcinoma in situ (LCIS) and infiltrating lobular carcinoma (ILC) with axillary metastases (META). Seven nuclear parameters were assessed, such as perimeter, area, shape factor, diameter of circle of equivalent area, longest axis, shortest axis and axes ratio. In comparison with nuclei of normal lobules, a statistically significant increase in the mean values of 5 nuclear parameters was observed in all pathologic lesions examined, whereas shape factor and axes ratio exhibited no significant difference. Nuclear area was found to be the most valuable parameter. No difference was observed between nuclear estimates of ALH and LCIS. Mean nuclear values were higher in ILC cases than in in-situ lesions, but no difference was detected between ILC and axillary metastases

Histological and ultrastructural evaluation of human decellularized matrix as a hernia repair device

Recently, interest has been increasing for human decellularized matrices, due to their ability to reduce numerous side effects related to hernia repair. To date, only animal studies investigated the biological interaction post-implant of human decellularized matrices for soft tissue repair. Therefore, the aim of this study was to evaluate the morphological response one year post implant of human decellularized matrix, through morphological analysis of human biopsies. The histological and ultrastructural results revealed a perfect cellular repopulation and neoangiogenesis, with minimal inflammatory response and a well-organized collagen matrix. The results have indicated that this scaffold can be an effective treatment for hernia

The use of an acellular matrix derived from human dermis for the treatment of full-thickness skin wounds.

Full-thickness skin wounds occur in many different clinical cases and the use of biological acellular dermal matrices (ADMs) to reconstruct the damaged area is increasing in the field of plastic and reconstructive surgery. In particular, the ability of ADMs to maintain the structural properties of extracellular matrix as well as to provide a suitable environment for cell growth makes their use suitable for the improvement of wound healing and the reduction of side effects deriving from contracture and scar tissue formation. In this study, we describe the clinical use of a recently developed human dermal matrix (HDM) in combination with graft skin as an alternative reconstructive solution for the treatment of full-thickness skin wounds. The HDM was applied in combination with autologous graft skin on three different clinical cases in which full-thickness skin wounds occurred. The clinical outcomes were evaluated in the patients during their follow-up. Histological as well as ultra-structural analysis were also performed on skin biopsy of the clinical case 3 one year after the treatment with HDM. The use of HDM stimulates the wound healing process in all clinical cases of full-thickness skin wounds here described with a functional and aesthetic rescue of the damaged area. Histological and ultra-structural analysis show a regenerative healing of the wound area with well-organized/oriented connective tissue in which cellular infiltration as well as blood vessels are evident. Our results support the clinical use of HDM as a permanent dermal replacement for the treatment of full-thickness skin wounds