Gut bacteria and necrotizing enterocolitis: cause or effect?

Development of necrotising enterocolitis (NEC) is considered to be dependent on the bacterial colonisation of the gut. With little concordance between published data and a recent study failing to detect a common strain in infants with NEC, more questions than answers are arising about our understanding of this complex disease

Social Vulnerability to Large Wildfires in the Western USA

Federal land managers in the US can be informed with quantitative assessments of the social conditions of the populations affected by wildfires originating on their administered lands in order to incorporate and adapt their management strategy to achieve a more targeted prioritization of community wildfire protection investments. In addition, these assessments are valuable to socially vulnerable communities for quantifying their exposure to wildfires originating on adjacent land tenures. We assessed fire transmission patterns using fire behavior simulations to understand spatial variations across three diverse study areas (North-central Washington; Central California; and Northern New Mexico) to understand how different land tenures affect highly socially vulnerable populated places. Transboundary wildfire structure exposure was related to populations with limited adaptive capacity to absorb, recover and modify exposure to wildfires, estimated with the Social Vulnerability Index using US Census unit data (block groups). We found geographic heterogeneity in terms of land tenure composition and estimated fire exposure. Although high social vulnerability block groups covered small areas, they had high population and structure density and were disproportionately exposed per area burned by fire. Structure exposure originated primarily from three key land tenures (wildland-urban interface, private lands and national forests). Federal lands proportionately exposed, on an area basis, populated places with high social vulnerability, with fires ignited on Forest Service administered lands mostly affecting north-central Washington and northern New Mexico communities

Tracking large carnivore dispersal using isotopic clues in claws: An application to cougars across the Great Plains

1. Cougar (Puma concolor) populations, like other large carnivores, have increased during recent decades and may be recolonizing their former ranges in Midwestern North America. The dispersal routes taken by these animals from established populations are unknown and insight into these movements would facilitate their conservation and management. 2. We inferred the origin and migration route of four dispersing cougars using stable hydrogen (δD) and carbon (δ13C) isotope values along one of their claws. We compared isotopic variations within claws to regional and large-scale isoscapes of δD and δ13C values in prey species. Using a likelihood-based assignment approach, we predicted the most likely dispersal route of each cougar (among several least-cost dispersal paths to potential source populations) in a chronological sequence dating back from its final location. 3. Our model predicted the origin of a radio-collared short-distance disperser and inferences about the most likely dispersal corridors for two long-distance dispersers matched reported information from re-sighting events and genetic investigations. 4. Insights about the most likely migration corridors may help identify critical areas and guide future conservation efforts of cougars and other large carnivores. We encourage managers to extend regional isoscapes based on sedentary prey species as they prove to be valuable tools in isotopic tracking of long-distance migration. 5. Our isotopic approach may be extended to other metabolically inert tissues that grow continuously, to investigate dispersal paths of species of interest, providing that individuals disperse across known isotopically structured landscapes

Ergebnisse der Begleitforschung zum Singangebot «Singen kennt kein Alter»

Beim kostenlosen Singangebot «Singen kennt kein Alter» (SikkA) besuchen freiwillige Singteams alle zwei Wochen Alters- und Pflegeheime in der Region Bern und singen mit den Bewohnenden. Die Singteams bestehen aus drei bis vier Personen über 65 Jahren, die jeweils ein Heim betreuen. Aktuell wird SikkA an drei Standorten angeboten. Der Trägerverein SikkA beauftragte das Institut Alter der Berner Fachhochschule im Jahr 2022 mit der wissenschaftlichen Begleitung des Singangebots. Ziel war es die Perspektive der Freiwilligen, Heimvertreter*innen und SikkA-Organisatorinnen zu sammeln und zu analysieren. Die Beteiligten äusserten sich zu bewährten Methoden für die Durchführung der Singstunden und zur Bedeutung von SikkA für sie selbst

Protein Replacement Therapy Partially Corrects the Cholesterol-Storage Phenotype in a Mouse Model of Niemann-Pick Type C2 Disease

Niemann-Pick type C2 (NPC2) disease is a fatal autosomal recessive neurovisceral degenerative disorder characterized by late endosomal-lysosomal sequestration of low-density lipoprotein derived cholesterol. The breach in intracellular cholesterol homeostasis is caused by deficiency of functional NPC2, a soluble sterol binding protein targeted to the lysosomes by binding the mannose-6-phosphate receptor. As currently there is no effective treatment for the disorder, we have investigated the efficacy of NPC2 replacement therapy in a murine gene-trap model of NPC2-disease generated on the 129P2/OlaHsd genetic background. NPC2 was purified from bovine milk and its functional competence assured in NPC2-deficient fibroblasts using the specific cholesterol fluorescent probe filipin. For evaluation of phenotype correction in vivo, three-week-old NPC2−/− mice received two weekly intravenous injections of 5 mg/kg NPC2 until trial termination 66 days later. Whereas the saline treated NPC2−/− mice exhibited massive visceral cholesterol storage as compared to their wild-type littermates, administration of NPC2 caused a marked reduction in cholesterol build up. The histological findings, indicating an amelioration of the disease pathology in liver, spleen, and lungs, corroborated the biochemical results. Little or no difference in the overall cholesterol levels was observed in the kidneys, blood, cerebral cortex and hippocampus when comparing NPC2−/− and wild type mice. However, cerebellum cholesterol was increased about two fold in NPC2−/− mice compared with wild-type littermates. Weight gain performance was slightly improved as a result of the NPC2 treatment but significant motor coordination deficits were still observed. Accordingly, ultrastructural cerebellar abnormalities were detected in both saline treated and NPC2 treated NPC2−/− animals 87 days post partum. Our data indicate that protein replacement may be a beneficial therapeutic approach in the treatment of the visceral manifestations in NPC2 disease and further suggest that neurodegeneration is not secondary to visceral dysfunction

“What if There's Something Wrong with Her?”‐How Biomedical Technologies Contribute to Epistemic Injustice in Healthcare

While there is a steadily growing literature on epistemic injustice in healthcare, there are few discussions of the role that biomedical technologies play in harming patients in their capacity as knowers. Through an analysis of newborn and pediatric genetic and genomic sequencing technologies (GSTs), I argue that biomedical technologies can lead to epistemic injustice through two primary pathways: epistemic capture and value partitioning. I close by discussing the larger ethical and political context of critical analyses of GSTs and their broader implications for just and equitable healthcare delivery