¹³C enrichment in plasma lactate of embryos incubated at two different eggshell temperatures (study III).

<p>¹³C enrichment in plasma lactate after injecting a single bolus of [U-¹³C]glucose (1.0 mg in 250 µL sterile water) at day 17.5 of incubation in the chorio-allantoic fluid of embryos incubated at a normal (37.8°C) or high (38.9°C) eggshell temperature (EST) from day 10.5 of incubation onward. *Significant difference between EST treatments (<i>P</i><0.05). At day 17.5, 17.8, 18.2, and 18.9 of incubation, no chickens were hatched yet. At day 19.7 of incubation, 22% of the chickens of the high EST treatment and 10% of the chickens of the normal EST treatment were hatched. At day 20.4 of incubation, 80% of the chickens of the high EST treatment were hatched and all chickens of the normal EST treatment were hatched. At day 20.9 and 21.6 of incubation, all chickens had been hatched.</p

Plasma uric acid concentration of embryos incubated at two different eggshell temperatures (study III).

<p>Plasma uric acid concentration of embryos incubated at a normal (37.8°C) or high (38.9°C) eggshell temperature (EST) from day 10.5 of incubation onward. *Significant difference between EST treatments (<i>P</i><0.05). At day 17.5, 17.8, 18.2, and 18.9 of incubation, no chickens were hatched yet. At day 19.7 of incubation, 22% of the chickens of the high EST treatment and 10% of the chickens of the normal EST treatment were hatched. At day 20.4 of incubation, 80% of the chickens of the high EST treatment were hatched and all chickens of the normal EST treatment were hatched. At day 20.9 and 21.6 of incubation, all chickens had been hatched.</p

¹³C enrichment in expired CO₂ of embryos incubated at two different eggshell temperatures (study III).

<p>¹³C enrichment in expired CO₂ after injecting a single bolus of [U-¹³C]glucose (1.0 mg in 250 µL sterile water) at day 17.5 of incubation in the chorio-allantoic fluid of embryos incubated at a normal (37.8°C) or high (38.9°C) eggshell temperature (EST) from day 10.5 of incubation onward. From day 17.6 until 17.8 of incubation,¹³C enrichment in expired CO₂ was higher for the high EST compared to the normal EST treatment (<i>P</i><0.05).</p

Embryo and chicken yolk-free body mass during incubation at two different eggshell temperatures (study III).

<p>Yolk-free body mass of embryos incubated at a normal (37.8°C) or high (38.9°C) eggshell temperature (EST) from day 10.5 of incubation onward. *Significant difference between EST treatments (<i>P</i><0.05). At day 17.5, 17.8, 18.2, and 18.9 of incubation, no chickens were hatched yet. At day 19.7 of incubation, 22% of the chickens of the high EST treatment and 10% of the chickens of the normal EST treatment were hatched. At day 20.4 of incubation, 80% of the chickens of the high EST treatment were hatched and all chickens of the normal EST treatment were hatched. At day 20.9 and 21.6 of incubation, all chickens had been hatched.</p

¹³C enrichment in plasma glucose of embryos incubated at two different eggshell temperatures (study III).

<p>¹³C enrichment in plasma glucose after injecting a single bolus of [U-¹³C]glucose (1.0 mg in 250 µL sterile water) at day 17.5 of incubation in the chorio-allantoic fluid of embryos incubated at a normal (37.8°C) or high (38.9°C) eggshell temperature (EST) from day 10.5 of incubation onward. At day 17.5, 17.8, 18.2, and 18.9 of incubation, no chickens were hatched yet. At day 19.7 of incubation, 22% of the chickens of the high EST treatment and 10% of the chickens of the normal EST treatment were hatched. At day 20.4 of incubation, 80% of the chickens of the high EST treatment were hatched and all chickens of the normal EST treatment were hatched. At day 20.9 and 21.6 of incubation, all chickens had been hatched.</p

¹³C in hepatic glycogen of embryos incubated at two different eggshell temperature (study III).

<p>¹³C in hepatic glycogen after injecting a single bolus of [U-¹³C]glucose (1.0 mg in 250 µL sterile water) at day 17.5 of incubation in the chorio-allantoic fluid of embryos incubated at a normal (37.8°C) or high (38.9°C) eggshell temperature (EST) from day 10.5 of incubation onward. *Significant difference between EST treatments (<i>P</i><0.05). At day 17.5, 17.8, 18.2, and 18.9 of incubation, no chickens were hatched yet. At day 19.7 of incubation, 22% of the chickens of the high EST treatment and 10% of the chickens of the normal EST treatment were hatched. At day 20.4 of incubation, 80% of the chickens of the high EST treatment were hatched and all chickens of the normal EST treatment were hatched. At day 20.9 and 21.6 of incubation, all chickens had been hatched.</p

¹³C enrichment in expired CO₂ in eggs incubated at two different eggshell temperatures (study I).

<p>¹³C enrichment in expired CO₂ after injecting a single bolus of [U-¹³C]glucose (1.0 mg in 250 µL sterile water) in the chorio-allantoic fluid at day 14.5 of incubation of embryos incubated at a normal (37.8°C) or high (38.9°C) eggshell temperature (EST) from day 13 of incubation onward.</p

¹³C enrichment in expired CO₂ of embryos incubated at two different eggshell temperatures (Study II).

<p>¹³C enrichment in expired CO₂, after injecting a single bolus of [U-¹³C]glucose (0.73 mg in 250 µL sterile water) for four consecutive days from day 14.5 of incubation in the chorio-allantoic fluid of embryos incubated at a normal (37.8°C) or high (38.9°C) eggshell temperature (EST) from day 13 of incubation onward.</p

Hepatic glycogen concentration of embryos incubated at two different eggshell temperatures (study III).

<p>Hepatic glycogen concentration of embryos incubated at a normal (37.8°C) or high (38.9°C) eggshell temperature (EST) from day 10.5 of incubation onward. *Significant difference between EST treatments (<i>P</i><0.05). At day 17.5, 17.8, 18.2, and 18.9 of incubation, no chickens were hatched yet. At day 19.7 of incubation, 22% of the chickens of the high EST treatment and 10% of the chickens of the normal EST treatment were hatched. At day 20.4 of incubation, 80% of the chickens of the high EST treatment were hatched and all chickens of the normal EST treatment were hatched. At day 20.9 and 21.6 of incubation, all chickens had been hatched.</p