407 research outputs found
Application of neural networks to the collision avoidance problem in 2D based on the TensorFlow library
The objective of this paper is the learning and initiation into the world of neural networks using the Google tool, TensorFlow. In order to do this, we consider a series of algorithms whose purpose is the control of a drone which can move in a specific environment, avoiding static and mobile obstacle while, at the same time, guaranteeing a safe navigation. This tool is the main different with respect to older research in this field. Furthermore, we look into the structure and the diverse tools that this platform offers with the intention of discovering the areas in which TensorFlow can be useful. Therefore, the organization of this paper is structured as follows:
In the first place, we offer an introduction that covers Neural Networks that are so important nowadays in the wide range of application available. We also explain what they are based on and how the information is used.
Next, TensorFlow structure is briefly introduced, explaining also how it works and some of the basics tools provided by it.
After that, the setting in which we are currently working is illustrated in three different steps. First, a data set is created, then the TensorFlow algorithms are implemented for the different scenarios and finally the "learning" obtain by the neural networks are analysed.
Lastly in our conclusions we offer two significant points: first, we demonstrate the findings of the different neural networks in the simulator provided and, second, the conclusions that we have reached in this paper and the future line of researches that this study put forth.Universidad de Sevilla. Grado en Ingeniería Electrónica, Robótica y Mecatrónic
Mutations and amplification of oncogenes in endometrial cancer
Alterations in oncogenes are critical steps in the development of endometrial cancer. To investigate the potential clinical relevance of the amplification of the oncogenes c-erbB2, c-myc, and int-2 and the mutation of K-ras in endometrial cancer, 112 tumors were examined using PCR-based fluorescent DNA technology. Amplification of the three oncogenes and the mutation of K-ras were correlated with age, tumor size, lymph node status, metastases, stage, histological types, grade, steroid hormone receptor expression (estrogen receptor, ER; progesterone receptor, PgR), family history of cancer, previous history of cancer or precursor lesions, and previous history of hormone replacement therapy. Oncogene amplification of c-erbB2 was detected in 18.9%, of c-myc in 2.7% and of int-2 in 4.2%, and K-ras mutation in 11.6%. No significant correlations could be detected between amplification of c-erbB2 and any of the other parameters. Mutation of K-ras is associated with positive expression of PgR. This might indicate that mutation and activation of K-ras are involved in the development of hormonal independence in endometrial cancer
Der Nachweis von isolierten Tumorzellen in Knochenmark und Blut von Patientinnen mit primärem Mammakarzinom – Standardisierte Methodik und klinische Relevanz
Mutation detection in familial and sporadic breast cancers by denaturing high-performance liquid chromatography (DHPLC)
Role of DNA methylation in miR-200c/141 cluster silencing in invasive breast cancer cells
<p>Abstract</p> <p>Background</p> <p>The miR-200c/141 cluster has recently been implicated in the epithelial to mesenchymal transition (EMT) process. The expression of these two miRNAs is inversely correlated with tumorigenicity and invasiveness in several human cancers. The role of these miRNAs in cancer progression is based in part on their capacity to target the EMT activators ZEB1 and ZEB2, two transcription factors, which in turn repress expression of E-cadherin. Little is known about the regulation of the mir200c/141 cluster, whose targeting has been proposed as a promising new therapy for the most aggressive tumors.</p> <p>Findings</p> <p>We show that the miR-200c/141 cluster is repressed by DNA methylation of a CpG island located in the promoter region of these miRNAs. Whereas in vitro methylation of the miR-200c/141 promoter led to shutdown of promoter activity, treatment with a demethylating agent caused transcriptional reactivation in breast cancer cells formerly lacking expression of miR-200c and miR-141. More importantly, we observed that DNA methylation of the identified miR-200c/141 promoter was tightly correlated with phenotype and the invasive capacity in a panel of 8 human breast cancer cell lines. In line with this, in vitro induction of EMT by ectopic expression of the EMT transcription factor Twist in human immortalized mammary epithelial cells (HMLE) was accompanied by increased DNA methylation and concomitant repression of the miR-200c/141 locus.</p> <p>Conclusions</p> <p>The present study demonstrates that expression of the miR-200c/141 cluster is regulated by DNA methylation, suggesting epigenetic regulation of this miRNA locus in aggressive breast cancer cell lines as well as untransformed mammary epithelial cells. This epigenetic silencing mechanism might represent a novel component of the regulatory circuit for the maintenance of EMT programs in cancer and normal cells.</p
Evaluation of HER2 expression in urothelial carcinoma cells as a biomarker for circulating tumor cells
Background
Detection of circulating tumor cells (CTC) by techniques based on epithelial cell adhesion molecule (EpCAM) is suboptimal in urothelial carcinoma (UC). As HER2 is thought to be broadly expressed in UC, we explored its utility for CTC detection.
Methods
HER2 and EpCAM expression was analyzed in 18 UC cell lines (UCCs) by qRT-PCR, western blot and fluorescence-activated cell scanning (FACS) and compared to the strongly HER2-expressing breast cancer cell line SKBR3 and other controls. HER2 expression in UC patient tissues was measured by qRT PCR and correlated with data on survival and risk for metastasis. UCCs with high EpCAM and variable HER2 expression were used for spike-in experiments in the CellSearch system. Twenty-one blood samples from 13 metastatic UC patients were analyzed for HER2-positive CTCs with CellSearch.
Results
HER2 mRNA and protein were broadly expressed in UCC, with some heterogeneity, but at least 10-fold lower than in the HER-2+ SKBR3 cells. Variations were unrelated to cellular phenotype or clinicopathological characteristics. EpCAM expression was essentially restricted to UCCs with epitheloid phenotypes. Heterogeneity of EpCAM and HER2 expression was observed also in spike-in experiments. The 7 of 21 blood samples from 6 of 13 patients were enumerated as CTC positive via EpCAM, but only one sample stained weakly positive (1+) for HER2.
Conclusions
Detection rate of CTCs by EpCAM in UC is poor, even in metastatic patients. Because of its widespread expression, particularly in patients with high risk of metastasis, detection of HER2 could improve identification of UC CTCs, which is why combined detection using antibodies for EpCAM and HER2 may be beneficial
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