Filipe Carreira da Silva, Mead and Modernity. Science, Selfhood and Democratic Politics

George H. Mead: A Therapy for the Malaise of Modernity? In his recent volume on Mead, Filipe Carreira da Silva proposes an interpretation of the pragmatist’s thought that develops through three fundamental points of reference. According to the author, science, selfhood and democratic politics constitute “the pillars” of a new approach to the problem of modernity; an approach in which the mutual interchange between these moments projects on the theoretical level a reflection of the relational ..

Individuality

Emerson’s thought played an important and well recognized role in the development of American pragmatism, but the role exerted by the Transcendentalist in some other philosophical European contexts was even more direct and incisive. On some circumstances, the “adventure” of Emerson’s seminal ideas carried out a journey to/from Europe whose “rebound effect” on the American context is useful to evaluate. The widely attested influence of Emerson on Nietzsche sets an expressive example of this outward/inwards journey; other plain indications suggest now to reconsider the Emerson-Bergson relation and the peculiar outcomes that this relation produced in James’ pragmatism. In this paper, the controversy between William James and Henri Bergson is considered as from the topic of individuality and the very similar way in which the two philosophers theorized its temporalistic and pluralistic constitution. Both of them have been deeply influenced by Emerson, even though in different measure and sometimes with different – if not diverging – philosophical results. Nevertheless, just their common reference to Emerson can contribute to a better understanding of their controversial relation and of the consequences of this relation on the development of James’ ethical views

The Universal Meanings of Common Discourse

A critical and aware return to pragmatism entails a preliminary focus upon the possibility of productive communication and a possible exchange among fields of research often far apart in terms of methods and spheres of application. This difficulty is felt all the more strongly if we refer to the contested intellectual legacy of George H. Mead, one often divided between opposing and conflicting fields of investigation. In this paper, I propose a reinterpretation of his thought that I believe could operate transversally. To do so, I interpret Mead’s idea of human creativity in a more radical way compared with some previous interpretations. This way, it is possible to see the constructive role of the relation between inter-subjective and intra-subjective communication as constitutive moments in an ethical self-construction of human beings. Finally, the peculiarity of human creativity, in all its complexity, shows itself as the starting point for responsible, normatively oriented conduct. Moreover, a new anthropological paradigm, also emerging from Mead’s suggestions, could become a source of inspiration for a renewed pattern of civic life and democratic citizenship

The gustin (CA6) gene polymorphism, rs2274333 (A/G), as a mechanistic link between PROP tasting and fungiform taste papilla density and maintenance

Taste sensitivity to PROP varies greatly among individuals and is associated with polymorphisms in the bitter receptor gene TAS2R38, and with differences in fungiform papilla density on the anterior tongue surface. Recently we showed that the PROP non-taster phenotype is strongly associated with the G variant of polymorphism rs2274333 (A/G) of the gene that controls the salivary trophic factor, gustin. The aims of this study were 1) to investigate the role of gustin gene polymorphism rs2274333 (A/G), in PROP sensitivity and fungiform papilla density and morphology, and 2) to investigate the effect of this gustin gene polymorphism on cell proliferation and metabolic activity. Sixty-four subjects were genotyped for both genes by PCR techniques, their PROP sensitivity was assessed by scaling and threshold methods, and their fungiform papilla density, diameter and morphology were determined. In vitro experiments examined cell proliferation and metabolic activity, following treatment with saliva of individuals with and without the gustin gene mutation, and with isolated protein, in the two iso-forms. Gustin and TAS2R38 genotypes were associated with PROP threshold (p=0.0001 and p=0.0042), but bitterness intensity was mostly determined by TAS2R38 genotypes (p<0.000001). Fungiform papillae densities were associated with both genotypes (p<0.014) (with a stronger effect for gustin; p=0.0006), but papilla morphology was a function of gustin alone (p<0.0012). Treatment of isolated cells with saliva from individuals with the AA form of gustin or direct application of the active iso-form of gustin protein increased cell proliferation and metabolic activity (p<0.0135). These novel findings suggest that the rs2274333 polymorphism of the gustin gene affects PROP sensitivity by acting on fungiform papilla development and maintenance, and could provide the first mechanistic explanation for why PROP super-tasters are more responsive to a broad range of oral stimul

Gastro-intestinal parasites of pigs in Sardinia: a copromicroscopical investigation

This paper illustrates a copromicroscopical investigation carried out in Sardinia to update epidemiological data on diffusion of gastro-intestinal parasites in swine. Results obtained lead to suggest the employment of copromicroscopic exam to monitorate parasites diffusion in swine breedings in order to set up correct prophylactic and therapeutically intervents

Identification of two new repetitive elements and chromosomal mapping of repetitive DNA sequences in the fish Gymnothorax unicolor (Anguilliformes: Muraenidae)

Muraenidae is a species-rich family, with relationships among genera and species and taxonomy that have not been completely clarified. Few cytogenetic studies have been conducted on this family, and all of them showed the same diploid chromosome number (2n=42) but with conspicuous karyotypic variation among species. The Mediterranean moray eel Gymnothorax unicolor was previously cytogenetically studied using classical techniques that allowed the characterization of its karyotype structure and the constitutive heterochromatin and argyrophilic nucleolar organizer regions (Ag-NORs) distribution pattern. In the present study, we describe two new repetitive elements (called GuMboI and GuDdeI) obtained from restricted genomic DNA of G. unicolor that were characterized by Southern blot and physically localized by in situ hybridization on metaphase chromosomes. As they are highly repetitive DNA sequences, they map in heterochromatic regions. However, while GuDdeI was localized in the centromeric regions, the GuMboI fraction was distributed on some centromeres and was co-localized with the nucleolus organizer region (NOR). Comparative analysis with other Mediterranean species such as Muraena helena pointed out that these DNA fractions are species-specific and could potentially be used for species discrimination. As a new contribution to the karyotype of this species, we found that the major ribosomal genes are localized on acrocentric chromosome 9 and that the telomeres of each chromosome are composed of a tandem repeat derived from a poly-TTAGGG DNA sequence, as it occurs in most vertebrate species. The results obtained add new information useful in comparative genomics at the chromosomal level and contribute to the cytogenetic knowledge regarding this fish family, which has not been extensively studied

Transcutol® p containing slns for improving 8-methoxypsoralen skin delivery

Topical psoralens plus ultraviolet A radiation (PUVA) therapy consists in the topical application of 8-methoxypsoralen (8-MOP) followed by the skin irradiation with ultraviolet A radiation. The employment of classical 8-MOP vehicles in topical PUVA therapy is associated with poor skin deposition and weak skin permeability of psoralens, thus requiring frequent drug administration. The aim of the present work was to formulate solid lipid nanoparticles (SLNs) able to increase the skin permeation of 8-MOP. For this purpose, the penetration enhancer Transcutol® P (TRC) was added to the SLN formulation. SLNs were characterized with respect to size, polydispersity index, zeta potential, entrapment efficiency, morphology, stability, and biocompatibility. Finally, 8-MOP skin diffusion and distribution within the skin layers was investigated using Franz cells and newborn pig skin. Freshly prepared nanoparticles showed spherical shape, mean diameters ranging between 120 and 133 nm, a fairly narrow size distribution, highly negative ζ potential values, and high entrapment efficiency. Empty and loaded formulations were almost stable over 30 days. In vitro penetration and permeation studies demonstrated a greater 8-MOP accumulation in each skin layer after SLN TRC 2% and TRC 4% application than that after SLN TRC 0% application. Finally, the results of experiments on 3T3 fibroblasts showed that the incorporation of TRC into SLNs could enhance the cellular uptake of nanoparticles, but it did not increase their cytotoxicity

Evolution of satellite DNA sequences in two tribes of Bovidae: a cautionary tale

Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experiments showed completely coherent results: the Bovini probe Bt1 hybridized only to members of the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1 hybridized only to members of the Caprini tribe and not to members of Bovini. Hybridization signals were detected in the heterochromatic regions of every acrocentric autosome, except for two pairs of autosomes from Capra hircus that did not show hybridization to probe Om1. No signal was detected on X and Y chromosomes or on bi-armed autosomes. Remarkably, probe Om1 showed almost 100% homology with a bacterial sequence reported in Helicobacter pylor

Some remarks on the use of TaqI to detect highly repetitive DNA sequences in human chromosomes

In the attempt to conclude investigation of the action of restriction endonucleases on eukaryote chromosomes, we carried out a series of experiments digesting in situ human metaphase chromosomes with AluI/TaqI followed by Giemsa staining. We focused on the centromeric regions of chromosomes1, 2 and 16 and noted that those areas appeared as intensely stained blocks after AluI digestion, but were dramatically reduced in size or completely destroyed after subsequent TaqI treatment. These results permitted us to draw some conclusions on the highly repetitive DNA composition of these regions, in terms of alphoid and classical satellite DNAs