Substitution patterns in the Neanderthal and <i>Streptomyces</i> rRNA genes.

<p>Substitution frequencies inferred from the (A) Neanderthal and (B) Streptomyces small and large subunit rRNA gene contigs. Complementary substitutions ratios are reported together giving six groups in total. Vertical bars indicate the estimated level of sequencing errors. Coverage of sequence reads for the (C, D) small subunit and (E, F) large subunit rRNA gene sequences from (C, E) Metazoa and (D, F) Streptomycetales.</p

Schematic overview of the analysis workflow.

<p>Schematic overview of the analysis workflow.</p

Phylogeny of rRNA gene sequences.

<p>The longest rRNA consensus sequence assembled from the Neanderthal data and assigned to Streptmycetales is shown in red (SSU_Streptomycetales C11). The rRNA gene sequences amplified from the cave sediment are shown in green colour. <i>Streptomyces coelicolor</i>, used as a reference in the alignment is shown in blue. The PCR-amplified sequences from the cave sediment are shown in green. “ACT primers clone A5_G07” refer to amplifications with the actinobacterial-specific primers, while the four sequences obtained from the universal primers 27f and 1492r are referred to as “universal primers clone u2_C02, 05, 08 and 09″. The phylogeny was inferred using the maximum likelihood method. Numbers refer to bootstrap support values higher than 75%.</p

Phylogeny of microbial collagenases.

<p>Collagenase consensus sequences are coloured in red (Contigs 108, 111–113). The actinobacterial clade is highlighted in yellow and subfamilies M09A and B are indicated. The <i>Streptomyces</i> reference sequences from the MEROPS M09 family are shown in green and the family holotypes in blue. MEROPS references displayed with species names and (arbitrary) collagenase copy number. The phylogeny was inferred using the maximum likelihood method. Numbers refer to bootstrap support values higher than 75%.</p

Phylogeny of prolyl aminopeptidases.

<p>Propyl aminopeptidase consensus sequences are coloured red (Contigs C1103–C1106). The clade containing contig C1104 and two <i>Streptomyces</i> species is shown in yellow. The <i>Streptomyces</i> reference sequences from MEROPS S33 family are shown in green and family holotypes in blue. MEROPS id is indicated for each sequence. The phylogeny was inferred using the maximum likelihood method. Numbers refer to bootstrap support values higher than 75%.</p

Food Patterns of Polish Older People

Abstract Food patterns of Polish older people were separated and described. The research included 422 people aged 65+ years, living in 5 geographical locations. Participants of the study were selected in quota sampling. Criteria for recruitment included sex, age (65-74 or 75+ years) and family status (living alone or living with other people). Respondents were asked questions about consumption of 55 food products. The factor analysis allowed for separating 21 food patterns. They included from 1 to 3 groups of products, intake of which was mutually dependant. Big number of separated food patterns and small number of products forming joint food patterns speak in advocacy of relatively smali reciprocal relationship between different food items consumed by the seniors in Poland