Photo-crosslinked alginate hydrogels support enhanced matrix accumulation by nucleus pulposus cells in vivo

SummaryObjectiveIntervertebral disc (IVD) degeneration is a major health concern in the United States. Replacement of the nucleus pulposus (NP) with injectable biomaterials represents a potential treatment strategy for IVD degeneration. The objective of this study was to characterize the extracellular matrix (ECM) assembly and functional properties of NP cell-encapsulated, photo-crosslinked alginate hydrogels in comparison to ionically crosslinked alginate constructs.MethodsMethacrylated alginate was synthesized by esterification of hydroxyl groups with methacrylic anhydride. Bovine NP cells were encapsulated in alginate hydrogels by ionic crosslinking using CaCl2 or through photo-crosslinking upon exposure to long-wave UV light in the presence of a photoinitiator. The hydrogels were evaluated in vitro by gross and histological analysis and in vivo using a murine subcutaneous pouch model. In vivo samples were analyzed for gene expression, ECM localization and accumulation, and equilibrium mechanical properties.ResultsIonically crosslinked hydrogels exhibited inferior proteoglycan accumulation in vitro and were unable to maintain structural integrity in vivo. In further studies, photo-crosslinked alginate hydrogels were implanted for up to 8 weeks to examine NP tissue formation. Photo-crosslinked hydrogels displayed temporal increases in gene expression and assembly of type II collagen and proteoglycans. Additionally, hydrogels remained intact over the duration of the study and the equilibrium Young's modulus increased from 1.24±0.09kPa to 4.31±1.39kPa, indicating the formation of functional matrix with properties comparable to those of the native NP.ConclusionsThese findings support the use of photo-crosslinked alginate hydrogels as biomaterial scaffolds for NP replacement

Dynamic Hydrostatic Pressure Promotes Differentiation of Human Dental Pulp Stem Cells

The masticatory apparatus absorbs high occlusal forces, but uncontrolled parafunctional or orthodontic forces damage periodontal ligament (PDL), cause pulpal calcification, pulp necrosis and tooth loss. Morphology and functional differentiation of connective tissue cells can be controlled by mechanical stimuli but effects of uncontrolled forces on intra-pulpal homeostasis and ability of dental pulp stem cells (DPSCs) to withstand direct external forces are unclear. Using dynamic hydrostatic pressure (HSP), we tested the hypothesis that direct HSP disrupts DPSC survival and odontogenic differentiation. DPSCs from four teenage patients were subjected to HSP followed by assessment of cell adhesion, survival and recovery capacity based on odontogenic differentiation, mineralization and responsiveness to bone morphogenetic protein-2 (BMP-2). HSP down-regulated DPSC adhesion and survival but promoted differentiation by increasing mineralization, in vivo hard tissue regeneration and BMP-2 responsiveness despite reduced cell numbers. HSP-treated DPSCs displayed enhanced odontogenic differentiation, an indication of favorable recovery from HSP-induced cellular stress

Susceptibility amplitude ratio for generic competing systems

We calculate the susceptibility amplitude ratio near a generic higher character Lifshitz point up to one-loop order. We employ a renormalization group treatment with $L$ independent scaling transformations associated to the various inequivalent subspaces in the anisotropic case in order to compute the ratio above and below the critical temperature and demonstrate its universality. Furthermore, the isotropic results with only one type of competition axes have also been shown to be universal. We describe how the simpler situations of $m$-axial Lifshitz points as well as ordinary (noncompeting) systems can be retrieved from the present framework.Comment: 20 pages, no figure

Energetic particle influence on the Earth's atmosphere

This manuscript gives an up-to-date and comprehensive overview of the effects of energetic particle precipitation (EPP) onto the whole atmosphere, from the lower thermosphere/mesosphere through the stratosphere and troposphere, to the surface. The paper summarizes the different sources and energies of particles, principally galactic cosmic rays (GCRs), solar energetic particles (SEPs) and energetic electron precipitation (EEP). All the proposed mechanisms by which EPP can affect the atmosphere are discussed, including chemical changes in the upper atmosphere and lower thermosphere, chemistry-dynamics feedbacks, the global electric circuit and cloud formation. The role of energetic particles in Earth’s atmosphere is a multi-disciplinary problem that requires expertise from a range of scientific backgrounds. To assist with this synergy, summary tables are provided, which are intended to evaluate the level of current knowledge of the effects of energetic particles on processes in the entire atmosphere

Hazard assessment of vegetated slopes

The hazard assessment of vegetated slopes are reviewed and discussed in terms of the stability of the slope both with and without vegetation, soil erosion and the stability of the vegetated slope from windthrow and snow loading. Slope stability can be determined by using either limit equilibrium or finite element stability analysis methods. The limit equilibrium methods are extended to incorporate the vegetation parameters that are important for the stability of a vegetated slope. The factors that contribute to soil erosion are reviewed and the techniques for assessing and measuring the rate of soil erosion are presented. The assessment of windthrow hazards are comprehensively discussed and a mechanistic model called ForestGALES is introduced which has flexibility for testing many different forest management scenarios. The hazards presented by snow loading on forested slopes are briefly reviewed

Hillslope processes: Mass wasting, slope stability and erosion

This chapter describes the dominant types of processes present on hillslopes where both gravity and running water are active. The impact of natural hillslope processes is important and is currently strongly influenced by human activity due to land use change and vegetation removal, and is becoming even greater due to climate change. Both the fundamentals of erosion and slope stability are discussed in this chapter with respect to processes, causes and impacts. To fully appreciate the role of vegetation in the remediation of adverse slope processes, the fundamentals of these slope processes are addressed. In the first part, the role of mass movements is discussed. The definitions used and physical principles underlying mass movements are explained and keys and diagnostic parameters are given to explain how to recognize certain types of mass movements in the field. The causes of mass movement are described, amongst which deforestation, adverse hydrological conditions or slope undercutting, are summarized. The main types of mass movements i.e. falls, slides and flows are then separately discussed, giving full details with regard to their causes, processes and consequences, as well as a first glimpse to the solutions to slope failure problems, which will be addressed in more detail elsewhere in the book. The second part addresses erosion processes. Accelerated erosion is considered as one of the greatest problems of land degradation as it removes the fertile topsoil at high rates. Mankind, who is removing the original vegetation for agricultural purposes, is causing this problem. Again the general principles behind soil erosion are illustrated, giving attention to the causes and the different soil erosion processes such as sheet erosion, rill and gully erosion, piping and tunnel erosion as well as tillage erosion

How vegetation reinforces soil on slopes

Once the instability process e.g. erosion or landslides has been identified on a slope, the type of vegetation to best reinforce the soil can then be determined. Plants improve slope stability through changes in mechanical and hydrological properties of the root-soil matrix. The architecture of a plants root system will influence strongly these reinforcing properties. We explain how root morphology and biomechanics changes between species. An overview of vegetation effects on slope hydrology is given, along with an update on the use of models to predict the influence of vegetation on mechanical and hydrological properties of soil on slopes. In conclusion, the optimal root system types for improving slope stability are suggested