Millennials Making Meanings: Social Constructions of Sexual Harassment regarding Gender and Power by Generation Y

The term sexual harassment was brought to light by legal scholar Catharine MacKinnon during the second wave feminist movement in the 1970s, and has since changed in its meaning over the past four decades, influencing policy, legal action, and the way we, as a society, treat this social problem. Millennials, or those born between 1980 and 2000, will be the next generation of working adults that will influence the way sexual harassment is understood and defined both legally and socially. The Millennial generation is typically considered liberal and socially conscious, prompting the research question of “How do Millennials socially construct sexual harassment in terms of gender and power?” Eighteen semi-structured interviews with adult Millennials up to age 33 were conducted. Analysis was informed by feminist theory, social constructionism, and critical race theory. Results showed while Millennials are quick to speak about inclusion of men as targets of sexual harassment, they did so at the cost of frankly discussing that women are targeted more often than men. Combined with the ability to discuss individual causes of sexual harassment compared to the structural, this led to my findings of“gender-blind” sexual harassment attitudes describing postfeminist beliefs among Millennials

Garden of Truth: The Prostitution and Trafficking of Native Women in Minnesota

Explores mental health needs of Native women working as prostitutes; their experience of sexual or physical violence and homelessness; use of shelters, rape crisis centers, and substance abuse treatment; and role of culture. Makes policy recommendations

Feasibility of Using Saltcedar as a Filler in Injection-Molded Polyethylene Composites

Saltcedar (Tamarix ramosissima) was investigated for use as a filler in wood-plastic composites (WPCs). The mineral content, water-soluble extractive content, and thermal stability of saltcedar flour were compared with those of a commercial pine wood flour. The wood flours were compounded with plastic, and the viscosities of the composite melts containing the two species were compared. Injection-molded composites produced from the compounded material were evaluated for mechanical performance and weatherability. Saltcedar flour had more minerals and water-soluble extractives than pine flour, which resulted in lower thermal stability, but also lower melt viscosity when compounded with high-density polyethylene. Injection-molded WPCs made from unextracted saltcedar performed similarly to those made from pine in accelerated weathering tests, but their mechanical properties were generally lower. The flexural modulus of elasticity increased when extracted wood flour was used, especially for the saltcedar composites. However, color stability and flexural strength changed little. Producing WPCs from these composites is possible, although economically feasible applications that use the advantageous properties of these species and that can tolerate or address the less desirable ones need to be identified and demonstrated

Waste-Wood-Derived Fillers for Plastics

Filled thermoplastic composites are stiffer, stronger, and more dimensionally stable than their unfilled counterparts. Such thermoplastics are usually provided to the end-user as a precompounded, pelletized feedstock. Typical reinforcing fillers are inorganic materials like talc or fiberglass, but materials derived from waste wood, such as wood flour and recycled paper fiber, are also effective as fillers. The goal of this project was to generate commercial interest in using waste-wood–paper-derived fillers (WPFs) to reinforce thermoplastics. The research strategy was twofold: developmental research and outreach. Specific objectives were (1) to improve wastepaper fiber preparation, feeding, and compounding methods, and optimize composite performance, and (2) to communicate to end-product manufacturers the advantages of WPF thermoplastics. The research was led and supported by the Forest Products Laboratory (FPL), with input from a consortium of 15 fiber suppliers and plastics manufacturers. Additional funding was provided by the Wisconsin Department of Natural Resources. Equipment was leased and installed at FPL. Eight general purpose formulations were developed—they included extrusion and injection molding grades of both polyethylene and polypropylene, reinforced with WPFs. An information packet containing performance data, appropriate processing conditions, sample pellets, sample parts, and a questionnaire was sent to nearly 500 commercial plastics manufacturers in Wisconsin, Illinois, and Michigan. In response to requests for in-house trials, FPL researchers conducted nearly 18 site visits. The researchers ensured proper handling of the material, provided consultation, and gathered information about processing and performance. The trials went very well, and parts were successfully manufactured at all facilities. Products included automobile trim components and housings, vacuum cleaner parts, paint brush handles, bicycle parts, cosmetic cases, and other household items. Great interest has been shown in the use of WPF thermoplastics; one consortium member is establishing a 4 million kg/yr (9 million lb/yr) facility. Total market demand is conservatively expected to exceed 45 million kg/yr (100 million lb/yr)

Organotypic cocultures as skin equivalents: A complex and sophisticated in vitro system

To assess the role of genes required for skin organogenesis, tissue regeneration and homeostasis, we have established in vitro skin equivalents composed of primary cells or cell lines, respectively. In these organotypic cocultures keratinocytes generate a normal epidermis irrespective of the species and tissue origin of fibroblasts. The combination of cells derived from mouse and human tissues facilitates the identification of the origin of compounds involved in epidermal tissue reconstitution and thus the precise analysis of growth regulatory mechanisms

Limiting the Persistence of a Chromosome Break Diminishes Its Mutagenic Potential

To characterize the repair pathways of chromosome double-strand breaks (DSBs), one approach involves monitoring the repair of site-specific DSBs generated by rare-cutting endonucleases, such as I-SceI. Using this method, we first describe the roles of Ercc1, Msh2, Nbs1, Xrcc4, and Brca1 in a set of distinct repair events. Subsequently, we considered that the outcome of such assays could be influenced by the persistent nature of I-SceI-induced DSBs, in that end-joining (EJ) products that restore the I-SceI site are prone to repeated cutting. To address this aspect of repair, we modified I-SceI-induced DSBs by co-expressing I-SceI with a non-processive 3′ exonuclease, Trex2, which we predicted would cause partial degradation of I-SceI 3′ overhangs. We find that Trex2 expression facilitates the formation of I-SceI-resistant EJ products, which reduces the potential for repeated cutting by I-SceI and, hence, limits the persistence of I-SceI-induced DSBs. Using this approach, we find that Trex2 expression causes a significant reduction in the frequency of repair pathways that result in substantial deletion mutations: EJ between distal ends of two tandem DSBs, single-strand annealing, and alternative-NHEJ. In contrast, Trex2 expression does not inhibit homology-directed repair. These results indicate that limiting the persistence of a DSB causes a reduction in the frequency of repair pathways that lead to significant genetic loss. Furthermore, we find that individual genetic factors play distinct roles during repair of non-cohesive DSB ends that are generated via co-expression of I-SceI with Trex2

Treg-selective IL-2 starvation synergizes with CD40 activation to sustain durable responses in lymphoma models

BACKGROUND: Roughly half of all diffuse large B-cell lymphomas (DLBCLs) are infiltrated by large numbers of regulatory T-cells (Tregs). Although the presence of 'effector' Tregs in particular is associated with an inferior prognosis in patients on standard rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP) immunochemotherapy, the role of this cell type during lymphoma initiation and progression is poorly understood. METHODS: Here, we use tissue microarrays containing prospectively collected DLBCL patient specimens, as well as data from publicly available cohorts to explore the mutational landscape of Treg-infiltrated DLBCL. We further take advantage of a model of MYC-driven lymphoma to mechanistically dissect the contribution of Tregs to lymphoma pathogenesis and to develop a strategy of Treg-selective interleukin-2 (IL-2) starvation to improve immune control of MYC-driven lymphoma. RESULTS: We find that all genetic DLBCL subtypes, except for one characterized by co-occurring MYD88/CD79 mutations, are heavily infiltrated by Tregs. Spectral flow cytometry and scRNA-sequencing reveal the robust expression of functional and immunosuppressive markers on Tregs infiltrating MYC-driven lymphomas; notably, we find that intratumoral Tregs arise due to local conversion from naïve CD4$^{+}$ precursors on tumor contact. Treg ablation in Foxp3$^{iDTR}$ mice, or by antibody-mediated Treg-selective blockade of IL-2 signaling, strongly reduces the lymphoma burden. We identify lymphoma B-cells as a major source of IL-2, and show that the effects of Treg depletion are reversed by the simultaneous depletion of Foxp3-negative CD4$^{+}$ T-cells, but not CD8$^{+}$ T-cells or natural killer (NK) cells. The inhibition of ATP hydrolyzation and adenosine production by Tregs at least partly phenocopies the effects of Treg depletion. Treg depletion further synergizes with pro-apoptotic CD40 activation to sustain durable responses. CONCLUSION: The combined data implicate Tregs as a potential therapeutic target in DLBCL, especially in combination with other immunotherapies

Identification of Hypoxia-Induced Genes in Human SGBS Adipocytes by Microarray Analysis

Hypoxia in adipose tissue is suggested to be involved in the development of a chronic mild inflammation, which in obesity can further lead to insulin resistance. The effect of hypoxia on gene expression in adipocytes appears to play a central role in this inflammatory response observed in obesity. However, the global impact of hypoxia on transcriptional changes in human adipocytes is unclear. Therefore, we compared gene expression profiles of human Simpson-Golabi-Behmel syndrome (SGBS) adipocytes under normoxic or hypoxic conditions to detect hypoxia-responsive genes in adipocytes by using whole human genome microarrays. Microarray analysis showed more than 500 significantly differentially regulated mRNAs after incubation of the cells under low oxygen levels. To gain further insight into the biological processes, hypoxia-regulated genes after 16 hours of hypoxia were classified according to their function. We identified an enrichment of genes involved in important biological processes such as glycolysis, response to hypoxia, regulation of cellular component movement, response to nutrient levels, regulation of cell migration, and transcription regulator activity. Real-time PCR confirmed eight genes to be consistently upregulated in response to 3, 6 and 16 hours of hypoxia. For adipocytes the hypoxia-induced regulation of these genes is shown here for the first time. Moreover in six of these eight genes we identified HIF response elements in the proximal promoters, specific for the HIF transcription factor family members HIF1A and HIF2A. In the present study, we demonstrated that hypoxia has an extensive effect on gene expression of SGBS adipocytes. In addition, the identified hypoxia-regulated genes are likely involved in the regulation of obesity, the incidence of type 2 diabetes, and the metabolic syndrome

A Journey to the West: The Ancient Dispersal of Rice Out of East Asia.

Funder: Max Planck Institute for the Science of Human HistoryRice is one of the most culturally valued and widely grown crops in the world today, and extensive research over the past decade has clarified much of the narrative of its domestication and early spread across East and South Asia. However, the timing and routes of its dispersal into West Asia and Europe, through which rice eventually became an important ingredient in global cuisines, has remained less clear. In this article, we discuss the piecemeal, but growing, archaeobotanical data for rice in West Asia. We also integrate written sources, linguistic data, and ethnohistoric analogies, in order to better understand the adoption of rice outside its regions of origin. The human-mediated westward spread of rice proceeded gradually, while its social standing and culinary uses repeatedly changing over time and place. Rice was present in West Asia and Europe by the tail end of the first millennium BC, but did not become a significant crop in West Asia until the past few centuries. Complementary historical, linguistic, and archaeobotanical data illustrate two separate and roughly contemporaneous routes of westward dispersal, one along the South Asian coast and the other through Silk Road trade. By better understanding the adoption of this water-demanding crop in the arid regions of West Asia, we explore an important chapter in human adaptation and agricultural decision making

RELICS: Strong Lens Models for Five Galaxy Clusters From the Reionization Lensing Cluster Survey

Strong gravitational lensing by galaxy clusters magnifies background galaxies, enhancing our ability to discover statistically significant samples of galaxies at z>6, in order to constrain the high-redshift galaxy luminosity functions. Here, we present the first five lens models out of the Reionization Lensing Cluster Survey (RELICS) Hubble Treasury Program, based on new HST WFC3/IR and ACS imaging of the clusters RXC J0142.9+4438, Abell 2537, Abell 2163, RXC J2211.7-0349, and ACT-CLJ0102-49151. The derived lensing magnification is essential for estimating the intrinsic properties of high-redshift galaxy candidates, and properly accounting for the survey volume. We report on new spectroscopic redshifts of multiply imaged lensed galaxies behind these clusters, which are used as constraints, and detail our strategy to reduce systematic uncertainties due to lack of spectroscopic information. In addition, we quantify the uncertainty on the lensing magnification due to statistical and systematic errors related to the lens modeling process, and find that in all but one cluster, the magnification is constrained to better than 20% in at least 80% of the field of view, including statistical and systematic uncertainties. The five clusters presented in this paper span the range of masses and redshifts of the clusters in the RELICS program. We find that they exhibit similar strong lensing efficiencies to the clusters targeted by the Hubble Frontier Fields within the WFC3/IR field of view. Outputs of the lens models are made available to the community through the Mikulski Archive for Space TelescopesComment: Accepted to Ap