3 research outputs found
Heterogeneity of hypothalamic pro-opiomelanocortin-expressing neurons revealed by single-cell RNA sequencing
Arcuate proopiomelanocortin (POMC) neurons are critical nodes in the control of body weight. Often characterized simply as direct targets for leptin, recent data suggest a more complex architecture.
Using single cell RNA sequencing, we have generated an atlas of gene expression in murine POMC neurons.
Of 163 neurons, 118 expressed high levels of with little/no Agrp expression and were considered “canonical” POMC neurons (P). The other 45/163 expressed low levels of and high levels of (AP). Unbiased clustering analysis of P neurons revealed four different classes, each with distinct cell surface receptor gene expression profiles. Further, only 12% (14/118) of P neurons expressed the leptin receptor () compared with 58% (26/45) of AP neurons. In contrast, the insulin receptor () was expressed at similar frequency on P and AP neurons (64% and 55%, respectively).
These data reveal arcuate POMC neurons to be a highly heterogeneous population. Accession Numbers: GSE92707.This work was supported by the UK Medical Research Council (MRC) Metabolic Disease Unit (MRC_MC_UU_12012/1 & MRC_MC_UU_12012/5), a Wellcome Trust Strategic Award (100574/Z/12/Z), and the Helmholtz Alliance ICEMED
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Heterogeneity of hypothalamic pro-opiomelanocortin-expressing neurons revealed by single-cell RNA sequencing
Arcuate proopiomelanocortin (POMC) neurons are critical nodes in the control of body weight. Often characterized simply as direct targets for leptin, recent data suggest a more complex architecture.
Using single cell RNA sequencing, we have generated an atlas of gene expression in murine POMC neurons.
Of 163 neurons, 118 expressed high levels of with little/no Agrp expression and were considered “canonical” POMC neurons (P). The other 45/163 expressed low levels of and high levels of (AP). Unbiased clustering analysis of P neurons revealed four different classes, each with distinct cell surface receptor gene expression profiles. Further, only 12% (14/118) of P neurons expressed the leptin receptor () compared with 58% (26/45) of AP neurons. In contrast, the insulin receptor () was expressed at similar frequency on P and AP neurons (64% and 55%, respectively).
These data reveal arcuate POMC neurons to be a highly heterogeneous population. Accession Numbers: GSE92707.This work was supported by the UK Medical Research Council (MRC) Metabolic Disease Unit (MRC_MC_UU_12012/1 & MRC_MC_UU_12012/5), a Wellcome Trust Strategic Award (100574/Z/12/Z), and the Helmholtz Alliance ICEMED