3CCD and infrared (IR) imaging. Top) Representative grayscale 3CCD images of a hind limb, and Middle) representative grayscale infrared images of a hind limb at (1) baseline, (2) maximum ischemia, (3) 10 minutes post-reperfusion, and (4) 30 minutes post-reperfusion.

<p>Bottom left) Profile of R-B values, derived from 3CCD imaging, over the course of 3.5 hours of ischemia and 30 minutes of reperfusion. Bottom right) Profile of IR values and corresponding mean leg temperature over the course of 3.5 hours of ischemia and 30 minutes of reperfusion. The time points mentioned previously (1–4) are noted.</p

Functional outcomes in sham and experimental ischemia groups.

<p>A) Mean drop foot scores for all experimental groups. A drop foot score of 0 meant that no neuropathy was observed post-operatively. A drop foot score of 1 was consistent with neuropathy in the affected limb for at least one day post-operatively. A drop foot score of 2 was consistent with neuropathy in the affected limb for at least three days post-operatively. A drop foot score of 3 was indicative of neuropathy in the affected limb beyond 5 days post-operatively. B) Mean Tarlov scores for all experimental groups. The dashed line at 5.0 is reflective of normal locomotion. The asterisks indicate statistically significant differences when compared to sham and 3.5 hour occlusion animals—** = p-value < 0.01, *** = p-value < 0.001.</p

Outcome based comparison of various metrics.

<p>Values are means +/- the standard deviation for animals that 1) recovered full locomotion, and 2) did not recover full locomotion. Differences approaching statistical significance (p-value ≈ 0.1) are indicated by (†). Statistically significant differences (p-value<0.05) are indicated by an asterisk (*).</p

Profiles of operative 3CCD and IR imaging values.

<p>Bar plots of mean R-B values derived from 3CCD imaging (A) and mean leg temperatures in °F (B) for all experimental groups at baseline, maximum ischemia, 10 minutes post-reperfusion, and 30 minutes post-reperfusion. Error bars = SEM (standard error of the mean). 3_5O = 3.5 hour occlusion; 3_5T = 3.5 hour tourniquet; 4_7O = 4.7 hour occlusion; 4_7T = 4.7 hour tourniquet.</p

Mean pathology scores for all experimental groups.

<p>Mean total pathology scores for all experimental groups: A) organs (kidneys and liver), and B) sciatic nerve and skeletal muscle. 3_5O = 3.5 hour occlusion; 3_5T = 3.5 hour tourniquet; 4_7O = 4.7 hour occlusion; 4_7T = 4.7 hour tourniquet.</p

Correlation of imaging parameters with blood chemistries.

<p>Pearson's correlation coefficients (ρ) for parameters involving blood chemistries. IR = infrared imaging; Rpf = Rpfusion; 3CCD = 3CCD imaging; PORH = post-occlusive reactive hyperemia; D = day.</p