Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil

A Gram-positive, spore-forming actinomycete strain (HKI0641T) was isolated from a soil sample collected in the Black Forest, Germany. During a screening for antimicrobial natural products this bacterium was identified as a producer of the antibiotic telomycin. Morphological characteristics and chemotaxonomic data suggested that the strain belongs to the genus Micromonospora. The peptidoglycan contains meso-diaminopimelic acid, and the fatty acid profile consists predominantly of anteiso-C15:0, iso-C15:0, iso-C16:0 and C16:0. MK-10(H4), MK-10(H2) and MK-10 were identified as the major menaquinones. To determine the taxonomic positioning of strain HKI0641T, we computed a binary tanglegram of two rooted phylogenetic trees that were based upon 16S rRNA and gyrB gene sequences, respectively. The comparative analysis of the two common classification methods strongly supported the phylogenetic affiliation with the genus Micromonospora, but it also revealed discrepancies in the assignment at the level of the genomic species. 16S rRNA gene sequence analysis identified M. coxensis DSM 45161T (99.1%) and M. marina DSM 45555T (99.0%) as the nearest taxonomic neighbours, whereas the gyrB sequence of strain HKI0641T indicated a closer relationship to M. aurantiaca DSM 43813T (95.1%). By means of DNA-DNA hybridization experiments, it was possible to resolve this issue and to clearly differentiate strain HKI0641T from other Micromonospora species. The type strains of the aforementioned Micromonospora species could be further distinguished from strain HKI0641T by several phenotypic properties, such as colony colour, NaCl tolerance and the utilization of carbon sources. The isolate was therefore assigned to a new species, for which the name Micromonospora schwarzwaldensis sp. nov. is proposed. The type strain is HKI0641T (= DSM 45708T = CIP 110415T).Fil: Vela Gurovic, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Hans-Knöll-Institute; AlemaniaFil: Müller, Sebastian. Hans-Knöll-Institute; AlemaniaFil: Domin, Nicole. Hans-Knöll-Institute; AlemaniaFil: Seccareccia, Ivana. Hans-Knöll-Institute; AlemaniaFil: Nietzsche, Sandor. University Hospital Jena; AlemaniaFil: Martin, Karin. Hans-Knöll-Institute; AlemaniaFil: Nett, Markus. Hans-Knöll-Institute; Alemani

Complete genome sequence of the filamentous gliding predatory bacterium Herpetosiphon aurantiacus type strain (114-95T)

Herpetosiphon aurantiacus Holt and Lewin 1968 is the type species of the genus Herpetosiphon, which in turn is the type genus of the family Herpetosiphonaceae, type family of the order Herpetosiphonales in the phylum Chloroflexi. H. aurantiacus cells are organized in filaments which can rapidly glide. The species is of interest not only because of its rather isolated position in the tree of life, but also because Herpetosiphon ssp. were identified as predators capable of facultative predation by a wolf pack strategy and of degrading the prey organisms by excreted hydrolytic enzymes. The genome of H. aurantiacus strain 114-95T is the first completely sequenced genome of a member of the family Herpetosiphonaceae. The 6,346,587 bp long chromosome and the two 339,639 bp and 99,204 bp long plasmids with a total of 5,577 protein-coding and 77 RNA genes was sequenced as part of the DOE Joint Genome Institute Program DOEM 2005

Transposition of a Ds element from a plasmid into the plant genome in Nicotiana plumbaginifolia protoplasts derived cells

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Some features about transposition of the maize element dissociation in Nicotiana plumbaginifolia

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Seedling lethality in Nicotiana plumbaginifolia conferred by Ds transposable element insertion into a plant-specific gene

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Herpetopanone, a diterpene from Herpetosiphon aurantiacus discovered by isotope labeling

The genome of the predatory bacterium Herpetosiphon aurantiacus 114-95T harbors a number of biosynthesis genes, including four terpene cyclase genes. To identify the terpenes biosynthesized from H. aurantiacus 114-95T, we fed the strain with 13C-labeled glucose and, subsequently, searched for characteristic mass shifts in its metabolome. This approach led to the discovery of a new natural product, of which the isotope pattern is indicative for a diterpene originating from the methylerythritol phosphate pathway. After large-scale fermentation of H. aurantiacus 114-95T, the putative diterpene was isolated in sufficient quantity to enable NMR-based structure elucidation. The compound, for which the name herpetopanone is proposed, features a rare octahydro-1H-indenyl skeleton. Herpetopanone bears resemblance to cadinane-type sesquiterpenes from plants, but is structurally entirely unprecedented in bacteria. Based on its molecular architecture, a possible biosynthetic pathway is postulated

Statistical molecular fragmentation: which parameters influence the branching ratios?

International audienceOur recently developed statistical model for molecular fragmentation (SMF) is used to study the decomposition of propane and to predict thermodynamically controlled yields of products as a function of excitation energy deposited into propane

Économie politique de la santé. Un exemple exemplaire

Du 9 au 12 juin 2015, la Revue de la régulation a été le partenaire privilégié du colloque international Recherche & Régulation « La théorie de la régulation à l’épreuve des crises » qui s’est tenu à Paris, à l’université Paris Diderot et à l’INALCO. Le colloque a réuni plus de 200 participants d’horizons très divers, avec notamment de fortes délégations venues d’Asie et notamment de Chine et du Japon. La revue aura l’occasion de revenir sur les contenus qui ont été présentés durant ce colloque, aux thématiques marquées par la dimension internationale. La Revue de la régulation publie dans le présent numéro un dossier thématique intitulé « Économie politique de la santé. Un exemple exemplaire », coordonné et introduit par Philippe Batifoulier et Jean-Paul Domin. >> lire la suite From 9 to 12 June 2015, the Revue de la régulation was the privileged partner of the International Conference on Research & Regulationon the theme of “The Theory of Regulation in Times of Crisis” which was held in Paris at Paris Diderot University and at the INALCO. The conference was attended by more than 200 delegates from highly varied horizons, with a particularly large contingent from Asia, notably from China and Japan. The research presented at this conference, the subjects of which were extremely international in nature, will be commented on in this review at a later date. This issue of the Régulation Review is devoted to a special feature entitled “The Political Economy of Health care. An exemplary example”, coordinated and presented by Philippe Batifoulier and Jean-Paul Domin. >> read mor