Relationships among the blood-feeding inhibition (BFI), the deterrence and the personal protection as measured in experimental hut trials.

<p>See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0170732#sec002" target="_blank">Methods</a> for details on the mathematical relationship among BFI, deterrence and personal protection. Values of BFI and deterrence from studies cited in the review by Strode <i>et al</i>. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0170732#pone.0170732.ref006" target="_blank">6</a>] have been plotted when both indicators can be extracted from Fig 2 and Table 12 of this review article.</p

Specific detection of <i>Plasmodium</i> DNA by real-time PCR in the artificial target mixtures.

<p>Footnote: Validation of real-time PCR on artificial mixed targets. Plasmids constructs are: Pf, Po and Pm for <i>P. falciparum, P. ovale</i> and <i>P. malariae</i> respectively. Corresponding detection systems primers/probe are shown as FAL, MAL, OVA and Plasmo. Data are cycle threshold (Ct) values.</p

Comparison of real-time PCR and ELISA-CSP for <i>Plasmodium spp</i> detection in a blind panel of mosquito samples.

<p>Footenote: A total of 43 and 22 positive samples were detected by real-time PCR in <i>An. gambiae</i> and <i>An. funestus</i> respectively. Real-time PCR did not confirm the ELISA-CSP results on 11 samples (9 in <i>An. gambiae</i> and 2 in <i>An. funestus</i>). ELISA –CSP was considered as a gold standard and the agreement between the two methods was “excellent” (κ = 0.8 and P<0.05 by Chi-square test).</p

Observed and expected proportions of mosquito counts according to Poisson, ZIP, NPMP and NB distributions.

<p>Observed and expected proportions of mosquito counts according to Poisson, ZIP, NPMP and NB distributions.</p

Primers and probes used for the detection and identification of <i>Plasmodium</i> species.

<p>Footenote:</p>a<p>Primers and probe sequences are as previously published <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052719#pone.0052719-Shokoples1" target="_blank">[7]</a>.</p>b<p>Probe sequence modified as previously published <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052719#pone.0052719-Diallo1" target="_blank">[26]</a>.</p>c<p>Primers sequences are as previously published <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052719#pone.0052719-Dana1" target="_blank">[27]</a>.</p>d<p>Primers sequences are as designed in this study.</p>e<p>TAMRA, 6-carboxytetramethylrhodamine; MGBNFQ, minor groove binding nonfluorescent quencher.</p

Absolute and relative quantification of <i>Plasmodium</i> DNA in mosquitoes.

<p>This figure shows a not significant difference was observed in the <i>P. falciparum</i> densities between the two <i>Anopheles</i> species (P-value = 0, 2197).</p

Upwind flight and localization indicators for the three genotypes in relation to treatment (Mean±95% Confidence Interval).

<p>***p<0.001, **p≤0.01, *p≤0.05, ns = not significant.</p

Estimations of the relationships between mosquito density and various geographical and environmental factors in OKT region according to the conditional NPMP model.

*<p>Difference between the mean value over all surveys and the value at a given survey</p