1 research outputs found
<sup>13</sup>C NMR and LC-MS Profiling of Stilbenes from Elicited Grapevine Hairy Root Cultures
Resveratrol and related oligostilbenes
are defense molecules produced
by grapevine in response to stresses including various elicitors or
signal molecules. Together with their prominent role <i>in planta,</i> these compounds have been the center of much attention in recent
decades due to their pharmacological properties. The cost-effective
production of resveratrol derivatives such as viniferins or more structurally
complex stilbene oligomers remains a challenging task. In this study,
the chemical diversity of stilbenes produced by <i>Vitis vinifera</i> Pinot Noir hairy roots was investigated after elicitation for 4
days with a mixture of methyl jasmonate (100 μM) and cyclodextrins
(50 mM). Two crude extracts obtained from the culture medium and from
the hairy roots were fractionated by centrifugal partition chromatography.
The fractions were chemically investigated by two complementary identification
approaches involving a <sup>13</sup>C NMR-based dereplication method
and liquid chromatography coupled to mass spectrometry (LC-MS). In
total, groups of 21 and 18 molecules, including flavonoids and stilbenes,
were detected in the culture medium and root extracts, respectively.
These included resveratrol monomers, dimers, trimers, and a tetramer,
thus highlighting the ability of elicited hairy root culture systems
to synthesize a wide diversity of secondary metabolites of pharmaceutical
significance. The main compounds were unambiguously identified as <i>trans</i>-resveratrol, ε-viniferin, <i>trans</i>-piceatannol, pallidol, scirpusin A, eriodictyol, naringenin, vitisin
B, and maackin