20 research outputs found
Vγ9Vδ2 T cells do not inhibit HIV replication in MoDC.
<p>MoDC were infected with HIV<sub>BAL</sub> and cultured with purified γδT cells stimulated or not stimulated with IPP. Before culture with γδT cells (DC+HIV T0), and after 5 days HIV p24 protein was tested in the supernatants by ELISA. Results from seven independent experiments are shown as Box and Whiskers: the box encompasses the interquartile range of individual measurements, the horizontal bar-dividing line indicates the median value, and the whiskers represents maximum and minimum values.</p
Vγ9Vδ2 T cells fail to induce CD86 and HLA-DR up-regulation on HIV-infected MoDC.
<p>MoDC were infected with HIV<sub>BAL</sub> and cultured with purified γδT cells for 5 days. Then, MoDC phenotype were evaluated by flow cytometry. (A) Representative histogram plots of one out of seven independent experiments showing CD86 expression on MoDC in the indicated conditions. (B) Induction of CD86 expression on MoDC by activated Vγ9Vδ2 T cells (fold of increase: IPP stimulated/not stimulated). (C) Representative histogram plots of one out of four independent experiments showing HLA-DR expression on MoDC in the indicated conditions. (D) HLA-DR expression on MoDC (mfi) in the indicated conditions. Results are shown as Box and Whiskers: the box encompasses the interquartile range of individual measurements, the horizontal bar-dividing line indicates the median value, and the whiskers represents maximum and minimum values.</p
HIV-infected MoDC fail to down-regulate CCR5.
<p>MoDC were infected with HIV<sub>BAL</sub> and cultured with purified γδT cells for 5 days. MoDC phenotype were evaluated by flow cytometry. (A) Representative histogram plots of one out of seven independent experiments showing CCR5 expression on MoDC in the indicated conditions. (B) Percentage of CCR5+ MoDC cultured with Vγ9Vδ2 T cells in the indicated conditions. Results are shown as Box and Whiskers: the box encompasses the interquartile range of individual measurements, the horizontal bar-dividing line indicates the median value, and the whiskers represents maximum and minimum values.</p
Effects of HIV-infected MoDC on Vγ9Vδ2 T cells proliferation.
<p>MoDC were infected with HIV<sub>BAL</sub> and cultured with CFDA-SE labeled Vγ9Vδ2 T cells. After 5 days, Vγ9Vδ2 T cells proliferation and activation was evaluated by flow cytometry. (A) Representative histogram plots of one out of seven independent experiments showing Vγ9Vδ2 T cells proliferation. (B) CD69 expression on Vγ9Vδ2 T cells in the indicated conditions. Vγ9Vδ2 T cells labeled with CFDA-SE were stimulated with IPP in the presence of MoDC infected or not with HIV<sub>BAL.</sub> After 5 days, Vγ9Vδ2 T cells proliferation was evaluated by flow cytometry. (C) Representative histogram plots of one out of seven independent experiments showing Vγ9Vδ2 T cells proliferation. (D) Percentage of proliferating Vγ9Vδ2 T cells upon IPP stimulation in the indicated conditions. (E) CD69 expression (mean fluorescence intensity, mfi) on IPP stimulated Vγ9Vδ2 T cells cultured with HIV infected or uninfected MoDC. Results are shown as Box and Whiskers: the box encompasses the interquartile range of individual measurements, the horizontal bar-dividing line indicates the median value, and the whiskers represents maximum and minimum values.</p
HIV-infected MoDC inhibit Vγ9Vδ2 T cells cytokines production.
<p>Vγ9Vδ2 T cells were stimulated with IPP in the presence of MoDC infected with HIV<sub>BAL.</sub> After 5 days, cytokines released in the supernatants were evaluated by a multiplex immunoassay. (A) IFN-γ, (B) TNF-α, and (C) MIP1-β production, in seven independent experiments, are shown as Box and Whiskers: the box encompasses the interquartile range of individual measurements, the horizontal bar-dividing line indicates the median value, and the whiskers represents maximum and minimum values.</p
HIV infection modulates Vδ1 and Vδ2 T-cells functionality.
<p>CD107a expression was analyzed in peripheral and in mucosal compartments in 3 P-HIV, 3 C-HIV patients and 3 HD by flow cytometry (Panel A). Basal IFNγ production by Vδ2 T-cells was analyzed in peripheral and in mucosal compartments in 4 P-HIV, 4 C-HIV patients and 4 HD by flow cytometry (Panel B). Data were considered significant with a P<0.05.</p
Vδ1 and Vδ2 T-cells in peripheral and mucosal compartments in HIV-patients and HD.
<p>Vδ1 and Vδ2 T-cells frequency was analyzed by flow cytometry in peripheral (Panels A-B) and in mucosal (Panels C-D) compartments of 15 P-HIV, 14 C-HIV patients and 35 HD. Data were considered significant with a p<0.05. Center line represents median; box represents interquartile range (IQR); whiskers represent range.</p
Clinical Characteristic of Patients at the Time of Blood Sampling.
<p><sup>a</sup>patients with >200 CD4 cells/mm<sup>3</sup>;</p><p><sup>b</sup>ND: Not Detected;</p><p><sup>c</sup>patients with <200 CD4 cells/mm<sup>3</sup>.</p><p>Clinical Characteristic of Patients at the Time of Blood Sampling.</p
Vδ1 T-cells differentiation in peripheral and mucosal compartments from HIV-patients and HD.
<p>The frequency of Naïve (CD45RA+CD27+, hatched bars), Central Memory (CD45RA-CD27+, white bars), Effector Memory (CD45RA-CD27-, grey bars) and Terminally differentiated (CD45RA+CD27-, black bars) Vδ1 T-cells cells was analysed in peripheral (Panel A) and mucosal (Panel B) compartments in 15 P-HIV, 14 C-HIV patients and 35 HD by flow cytometry. Data were considered significant with a P<0.05.</p
Clinical features of enrolled subjects.
<p>HD, healthy donors; NT, not tested;</p><p>* HIV therapy: pt1: lamivudine, zidovudine, nevirapine; pt2: atazanavir, ritonavir, tenofovir, emtricitabine; pt3: ritonavir, darunavir, tenofovir, emtricitabina.</p><p>Clinical features of enrolled subjects.</p