Ku55933 and NU7441 altered CaP cell lines cell cycle and increased apoptosis.

<p><b>LNCaP (A&C)</b> and <b>PC3 (B&D)</b> cells were incubated with Ku55933 (10 µM) and/or NU7441 (1 µM) before being exposed to the indicated doses of IR or doxorubicin for 48 h and then stained with PI and analysed directly on FACscan. Data is a representative three independent experiments. <b>LNCaP (E)</b> and <b>PC3 (F)</b> cells were treated as described above and stained with FITC-conjugated anti caspase 3 antibody before direct analysis on FACScan. Results are the mean of three independent experiments ± SD.</p

Ku55933 and NU7441 increased DNA DSB in CaP cell lines.

<p><b>LNCaP (A&C)</b> and <b>PC3 (B&D)</b> cells were treated with 10 Gy or 1 µM doxorubicin following 1 h incubation with Ku55933 (10 µM) and/or NU7441 (1 µM) and stained with FITC-conjugated H2AX antibody before being analysed directly on FACscan. Results are the mean of at least 5 independent experiments±SEM. <b>PC3 (E)</b> cells were treated as above for 24 h and analysed using comet assay, 50 cells were counted per arm treatment. Results are the mean of 3 independent experiments ± SEM.</p

Ku55933 and/or NU7441 sensitised CaP cells to IR or doxorubicin.

<p><b>LNCaP (A&B)</b> or <b>PC3 (C&D)</b> cells were seeded in different densities and left to attach before 1 h incubation with varying concentration of Ku55933 or NU7441 prior to irradiation 1 Gy or 10 nM doxorubicin treatment for 24 h (<i>P</i><0.001). <b>LNCaP (E&F)</b> or <b>PC3 (G&H)</b> cells were seeded in different densities and left to attach before 1 h incubation with Ku55933 (10 µM) or NU7441 (1 µM) prior to treatment with varying IR doses or doxorubicin concentration for 24 h (<i>P</i><0.001). All results are the mean of three independent experiments ± SEM.</p

Comparison between the characterisation results from immunoflourescent microscopy (IF) and Imagestream^X (IS^X).

<p>Comparison between the characterisation results from immunoflourescent microscopy (IF) and Imagestream^X (IS^X).</p

Brightfield images of PCO 162 demonstrating the effects of selective seeding.

<p>A: Epithelial cell growth from supernatant removed 30 minutes after initial plating (×20 magnification); B: Cells adhering during the initial 30 minute incubation were almost entirely fibroblastic (×10 magnification).</p

Growth curves for 6 representative PCO cultures (PCO143, 146, 149, 164,167 and 168).

<p>The mean and SD of 6 repeats for each time point is plotted, normalised to day 1.</p

Antigen expression in primary cultures.

<p>PCO 158 A: Immunohistochemical detection of CA125 in FFPE tissue; B: Immunofluourescent detection of CA125 with Alexaflour596 from corresponding primary culture</p

HR DNA repair status of PCO culture with corresponding sensitivity to 10 µM Rucaparib.

<p>*Cell survival following 10 µM Rucaprib. Resistant (R)  =  more than 70% survival; Sensitive (S)  =  less than 70% survival.</p

Figure 6

<p>A: ImagestreamX cell plot of representative cells demonstrating the three major cellular sub-populations within ascitic fluid, ; i) CK positive only, ii) CK and CA125 positive and iii) EpCAM, CK and CA125 positive. B: Sub-populations identified in the epithelial cell population in EOC ascitic fluid (n = the total number of epithelial cells identified in 10 ml of ascites).</p

Primary culture outcomes following transport.

<p>Primary culture outcomes following transport.</p