5 research outputs found
Basic Procedures in Administration of Zoning Regulations : Roles of Enforcement Officer and Board of Adjustment
The Zoning Enforcement Officer, who often has the title of Building Inspector, is the key person in the enforcement of the zoning regulations. If he or she does not thoroughly understand and carry out his or her duties, most of the effectiveness of zoning will be lost. The Zoning Enforcement Officer should read and become familiar with the zoning provisions. The officer should keep track of all amendments enacted by the legislative body, taking care to note them on his or her copy of the regulations, including, when necessary, the zoning district map. The officer should attend the meetings of the Board of Adjustment in order to understand the actions of the Board.Reviewed October 1993
Basic Procedures in Administration of Zoning Regulations Appeals
This guide has been prepared as a training aid for city and county officials and citizens. It illustrates the basic sequence of events in situations commonly involved with administration of local zoning regulations. It indicates the normal place and function of the various officials and bodies in the different kinds of procedures
Basic Procedures in Administration of Zoning Regulations : Making Applications
This guide has been prepared as a training aid for city and county officials and citizens. It illustrates the basic sequence of events in situations commonly involved with administration of local zoning regulations. It indicates the normal place and function of the various officials and bodies in the different kinds of procedures.Reviewed October 1993
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2′-O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography
The genome of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) coronavirus has a capping modification at the 5′-untranslated region (UTR) to prevent its degradation by host nucleases. These modifications are performed by the Nsp10/14 and Nsp10/16 heterodimers using S-adenosylmethionine as the methyl donor. Nsp10/16 heterodimer is responsible for the methylation at the ribose 2′-O position of the first nucleotide. To investigate the conformational changes of the complex during 2′-O methyltransferase activity, we used a fixed-target serial synchrotron crystallography method at room temperature. We determined crystal structures of Nsp10/16 with substrates and products that revealed the states before and after methylation, occurring within the crystals during the experiments. Here we report the crystal structure of Nsp10/16 in complex with Cap-1 analog (m7GpppAm2′-O). Inhibition of Nsp16 activity may reduce viral proliferation, making this protein an attractive drug target