Summary of vaccine responses.

<p>Summary of vaccine responses.</p

Multivariable model of seroconversion within those vaccinated in risk groups (chronic disease and/or immunocompromised).

<p>Multivariable model of seroconversion within those vaccinated in risk groups (chronic disease and/or immunocompromised).</p

Distribution of fold responses pre to post Pandemrix™ vaccination by 2009 seasonal influenza vaccination status.

<p>Distribution of fold responses pre to post Pandemrix™ vaccination by 2009 seasonal influenza vaccination status.</p

Summary of cohort for analysis.

<p>Summary of cohort for analysis.</p

Mortality Attributable to Influenza in England and Wales Prior to, during and after the 2009 Pandemic

<div><p>Very different influenza seasons have been observed from 2008/09–2011/12 in England and Wales, with the reported burden varying overall and by age group. The objective of this study was to estimate the impact of influenza on all-cause and cause-specific mortality during this period. Age-specific generalised linear regression models fitted with an identity link were developed, modelling weekly influenza activity through multiplying clinical influenza-like illness consultation rates with proportion of samples positive for influenza A or B. To adjust for confounding factors, a similar activity indicator was calculated for Respiratory Syncytial Virus. Extreme temperature and seasonal trend were controlled for. Following a severe influenza season in 2008/09 in 65+yr olds (estimated excess of 13,058 influenza A all-cause deaths), attributed all-cause mortality was not significant during the 2009 pandemic in this age group and comparatively low levels of influenza A mortality were seen in post-pandemic seasons. The age shift of the burden of seasonal influenza from the elderly to young adults during the pandemic continued into 2010/11; a comparatively larger impact was seen with the same circulating A(H1N1)pdm09 strain, with the burden of influenza A all-cause excess mortality in 15–64 yr olds the largest reported during 2008/09–2011/12 (436 deaths in 15–44 yr olds and 1,274 in 45–64 yr olds). On average, 76% of seasonal influenza A all-age attributable deaths had a cardiovascular or respiratory cause recorded (average of 5,849 influenza A deaths per season), with nearly a quarter reported for other causes (average of 1,770 influenza A deaths per season), highlighting the importance of all-cause as well as cause-specific estimates. No significant influenza B attributable mortality was detected by season, cause or age group. This analysis forms part of the preparatory work to establish a routine mortality monitoring system ahead of introduction of the UK universal childhood seasonal influenza vaccination programme in 2013/14.</p></div

Number of influenza A attributable all-cause deaths (95% Confidence Interval) by primary ICD-10 cause of death and influenza season with the dominant circulating influenza A subtype^*.

*<p>Bold figures correspond to significant estimates.</p>1<p>Acute Myocardial Infarction.</p

Number of all- cause influenza-attributable A and B deaths (95% Confidence Interval) and rate per 100,000 population by age group, influenza type and influenza season^*.

*<p>Bold figures correspond to significant estimates.</p

Proportion of all-cause deaths attributable to influenza A by age group and season when assessing coded causes of death.

<p>Proportion of all-cause deaths attributable to influenza A by age group and season where significant (corresponding confidence intervals not crossing 0). The number of influenza A-attributable deaths correspond to the following datasets analysed: all-cause deaths (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079360#pone-0079360-g003" target="_blank">Figure 3a</a>), cardiorespiratory deaths (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079360#pone-0079360-g003" target="_blank">Figure 3b</a>), respiratory deaths (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079360#pone-0079360-g003" target="_blank">Figure 3c</a>) and pneumonia and influenza deaths (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079360#pone-0079360-g003" target="_blank">Figure 3d</a>). Dominant influenza A subtype by season is indicated.</p

Additional file 1: of Elucidating the impact of the pneumococcal conjugate vaccine programme on pneumonia, sepsis and otitis media hospital admissions in England using a composite control

Identification of cases, comorbidities and control conditions; additional analyses of the incidence of cases and control conditions; sensitivity analysis; further analyses on the increased incidence of non-specific sepsis. (DOCX 260 kb

Number of cardiorespiratory coded influenza-attributable A and B deaths (95% Confidence Interval) and rate per 100,000 population by age group, influenza type and influenza season^*.

*<p>Bold figures correspond to significant estimates.</p