Bitter Compounds Decrease Gastric Emptying and Influence Intestinal Nutrient Transport

The effect of bitter tasting compounds on gastric emptying and nutrient transport from the intestine was studied using in vivo and ex vivo models. Sixteen pigs were fed a diet containing the bitter compound phenylthiocarbamide (PTC). The animals were euthanized 45 minutes postprandially and gastric contents were measured to quantify the gastric retention. Additionally, freshly isolated small intestines were mounted into modified Ussing chambers to study the effects of PTC on ex vivo nutrient transport. In summary, bitter compounds decreased the gastric emptying in vivo and increased the nutrient transport ex vivo. Further, cell culture studies identified that bitter compounds might exert their action through stimulating the secretion of the intestinal hormone cholecystokinin (CCK) from the enteroendocrine cells by increasing the intracellular calcium concentrations. Altogether, these data suggest that bitter compounds regulate feed intake and nutrient transport

Dietary oil composition differentially modulates intestinal endotoxin transport and postprandial endotoxemia

Background: Intestinal derived endotoxin and the subsequent endotoxemia can be considered major predisposing factors for diseases such as atherosclerosis, sepsis, obesity and diabetes. Dietary fat has been shown to increase postprandial endotoxemia. Therefore, the aim of this study was to assess the effects of different dietary oils on intestinal endotoxin transport and postprandial endotoxemia using swine as a model. We hypothesized that oils rich in saturated fatty acids (SFA) would augment, while oils rich in n-3 polyunsaturated fatty acids (PUFA) would attenuate intestinal endotoxin transport and circulating concentrations. Methods: Postprandial endotoxemia was measured in twenty four pigs following a porridge meal made with either water (Control), fish oil (FO), vegetable oil (VO) or coconut oil (CO). Blood was collected at 0, 1, 2, 3 and 5 hours postprandial and measured for endotoxin. Furthermore, ex vivo ileum endotoxin transport was assessed using modified Ussing chambers and intestines were treated with either no oil or 12.5% (v/v) VO, FO, cod liver oil (CLO), CO or olive oil (OO). Ex vivo mucosal to serosal endotoxin transport permeability (Papp) was then measured by the addition of fluorescent labeled-lipopolysaccharide. Results: Postprandial serum endotoxin concentrations were increased after a meal rich in saturated fatty acids and decreased with higher n-3 PUFA intake. Compared to the no oil control, fish oil and CLO which are rich in n-3 fatty acids reduced ex vivo endotoxin Papp by 50% (P \u3c 0.05). Contrarily, saturated fatty acids increased the Papp by 60% (P = 0.008). Olive and vegetable oils did not alter intestinal endotoxin Papp. Conclusion: Overall, these results indicate that saturated and n-3 PUFA differentially regulate intestinal epithelial endotoxin transport. This may be associated with fatty acid regulation of intestinal membrane lipid raft mediated permeability

Postprandial serum endotoxin in healthy humans is modulated by dietary fat in a randomized, controlled, cross-over study

Background: High-fat diets may contribute to metabolic disease via postprandial changes in serum endotoxin and inflammation. It is unclear how dietary fat composition may alter these parameters. We hypothesized that a meal rich in n-3 (ω3) fatty acids would reduce endotoxemia and associated inflammation but a saturated or n-6 (ω6) fatty acid-rich meal would increase postprandial serum endotoxin concentrations and systemic inflammation in healthy adults. Methods: Healthy adults (n = 20; mean age 25 ± 3.2 S.D. years) were enrolled in this single-blind, randomized, cross-over study. Participants were randomized to treatment and reported to the laboratory, after an overnight fast, on four occasions separated by at least one week. Participants were blinded to treatment meal and consumed one of four isoenergetic meals that provided: 1) 20 % fat (control; olive oil) or 35 % fat provided from 2) n-3 (ω3) (DHA = 500 mg; fish oil); 3) n-6 (ω6) (7.4 g; grapeseed oil) or 4) saturated fat (16 g; coconut oil). Baseline and postprandial blood samples were collected. Primary outcome was defined as the effect of treatment meal on postprandial endotoxemia. Serum was analyzed for metabolites, inflammatory markers, and endotoxin. Data from all 20 participants were analyzed using repeated-measures ANCOVA. Results: Participant serum endotoxin concentration was increased during the postprandial period after the consumption of the saturated fat meal but decreased after the n-3 meal (p  0.05). There was no treatment meal effect on participant postprandial serum biomarkers of inflammation. Postprandial serum triacylglycerols were significantly elevated following the n-6 meal compared to the n-3 meal. Non-esterified fatty acids were significantly increased after consumption of the saturated fat meal compared to other treatment meals. Conclusions: Meal fatty acid composition modulates postprandial serum endotoxin concentration in healthy adults. However, postprandial endotoxin was not associated with systemic inflammation in vivo. Trial registration: This study was retrospectively registered at clinicaltrials.gov as NCT02521779 on July 28, 2015

Effects of Heat Stress and Plane of Nutrition on Production and Metabolism in Growing Pigs

We heat-stressed growing pigs and evaluated the differential effects of environmental hyperthermia and reduced feed intake. Our results indicate that hyperthermia directly effects production and metabolism and many of these are independent of reduced nutrient intake. Identifying how and why heat stress alters metabolism and physiology are prerequisites in developing ameliorating strategies to prevent the reduction in performance and lost income typically observed during the warm summer months

How has selection for residual feed intake (RFI) affected the grow-finish pig’s ability to cope with stress?

Feed is the largest cost in pork production; therefore, improving feed efficiency can increase producer profitability. Furthermore improved feed efficiency can support industry competitiveness, decrease the demand on global feed resources, and complement environmental sustainability. Genetically, selective breeding for residual feed intake (RFI) shows promise in meeting these increased demands. However, it is important to balance the benefits of feed efficiency selection with the pig’s ability to cope with stress and its welfare. Therefore, this factsheet will discuss physiological and behavioral stress research on swine selected on the basis of RFI

Performance and Carcass Composition of Yorkshire Pigs Selected for Low Residual Feed Intake under Ad Libitum and Restricted Feeding

Growth performance and carcass composition of 40 Yorkshire pigs (74.8±9.9 kg or 164.9±21.8 lbs), 20 pigs from a line selected for low residual feed intake for 5 generations and 20 pigs from a control line, was observed while fed on either an ad libitum or NRC maintenance (weight-stasis) basis over a 6 week period. The aim of the latter diet treatment was to keep pigs at a constant weight for six weeks. In the ad libitum treatment, there was no difference in initial (p \u3c 0.49) or final body weights (p \u3c 0.65) but the low residual feed intake line consumed 9% less feed compared to the control (p \u3c 0.08). Similarly, there was no difference in LEA (p \u3c 0.57) but the low residual feed intake line had slightly less backfat compared to the control (p \u3c 0.21). These same results were found from chemical analysis of the carcass, as there was no difference in protein percentage (p \u3c 0.60), but the ad libitum low residual feed intake pigs had a slightly lower fat percentage (p \u3c 0.21). For the weight stasis treatment, the low residual feed intake pigs weighed 3.5% more than the control (p \u3c 0.08), despite attempts to maintain a static body weight, and consumed 7.6% less feed overall (p \u3c 0.09). Both lines had a decrease in backfat; however, the low residual feed intake line had an increase in loin eye area while the control line had a decrease. No differences were observed in chemical carcass composition between the two lines on the weight stasis treatment. These data show that the low residual feed intake line is more efficient, with only slight differences in carcass composition