Additional file 1 of Potential novel biomarkers in small intestine for obesity/obesity resistance revealed by multi-omics analysis

Supplementary Material

Characterization and Comparison of Dissolved Organic Matter Signatures in Steam-Assisted Gravity Drainage Process Water Samples from Athabasca Oil Sands

Steam-assisted gravity drainage (SAGD) process water contains high concentrations of dissolved organic and inorganic matter. A wide range of analytical techniques including electrospray ionization mass spectrometry, gas chromatography–mass spectrometry, Fourier transform infrared spectrometry, and fluorescence spectrophotometry have been utilized for the identification and measurement of dissolved organic matter (DOM) in oil sands process-affected water. The composition of DOM in the SAGD water is relatively complex, and thus one plausible method for its analysis is the fractionation of DOM into hydrophilic and hydrophobic portions using suitable resin columns and the characterization of these fractions using standard analytical methods. Comparing the fractionation and characterization of the SAGD produced water from different plant sites can provide considerable insight into better management, recycle, and reuse of this process water. Also, a detailed knowledge of the chemical composition of the SAGD produced water provides guidelines for identifying the constituents that are responsible for scaling and fouling at various stages of the SAGD process. This study aims at developing a systematic approach for the fractionation and characterization methods of SAGD process water samples

Integrated Omic Analysis of a Guinea Pig Model of Heart Failure and Sudden Cardiac Death

Here, we examine key regulatory pathways underlying the transition from compensated hypertrophy (HYP) to decompensated heart failure (HF) and sudden cardiac death (SCD) in a guinea pig pressure-overload model by integrated multiome analysis. Relative protein abundances from sham-operated HYP and HF hearts were assessed by iTRAQ LC–MS/MS. Metabolites were quantified by LC–MS/MS or GC–MS. Transcriptome profiles were obtained using mRNA microarrays. The guinea pig HF proteome exhibited classic biosignatures of cardiac HYP, left ventricular dysfunction, fibrosis, inflammation, and extravasation. Fatty acid metabolism, mitochondrial transcription/translation factors, antioxidant enzymes, and other mitochondrial procsses, were downregulated in HF but not HYP. Proteins upregulated in HF implicate extracellular matrix remodeling, cytoskeletal remodeling, and acute phase inflammation markers. Among metabolites, acylcarnitines were downregulated in HYP and fatty acids accumulated in HF. The correlation of transcript and protein changes in HF was weak (<i>R</i>² = 0.23), suggesting post-transcriptional gene regulation in HF. Proteome/metabolome integration indicated metabolic bottlenecks in fatty acyl-CoA processing by carnitine palmitoyl transferase (<i>CPT1B</i>) as well as TCA cycle inhibition. On the basis of these findings, we present a model of cardiac decompensation involving impaired nuclear integration of Ca²⁺ and cyclic nucleotide signals that are coupled to mitochondrial metabolic and antioxidant defects through the CREB/PGC1α transcriptional axis