2 research outputs found
Tumor Specific and Renal Excretable Star-like Triblock Polymer–Doxorubicin Conjugates for Safe and Efficient Anticancer Therapy
Efficient
tumor accumulation and body clearance are two paralleled
requirements for ideal nanomedicines. However, it is hard for both
to be met simultaneously. The inefficient clearance often restrains
the application of drug delivery systems (DDSs), especially for high-dosage
administration. In this study, the star-like and block structures
are combined to enhance the tumor specific targeting of the parent
structures and obtain additional renal excretion property. The influences
of polymer architectures and chemical compositions on the physicochemical
and biological properties, particularly the simultaneous achievement
of tumor accumulation and renal clearance, have been investigated.
Among the tested conjugates, an eight-arm triblock star polymer based
on polyÂ(ethylene glycol) (PEG) and polyÂ(<i>N</i>-(2-hydroxyl)
methacrylamide) (PHPMA) is found to simultaneously fulfill the requirements
of superior tumor accumulation and efficient renal clearance due to
the appropriate micelle size and reversible aggregation process. On
the basis of this conjugate, 60 mg/kg of Dox equivalent (much higher
than the maximum tolerated dose (MTD) of Dox) can be administered
to efficiently suppress tumor growth without causing any obvious toxicity.
This work provides a new approach to design polymer–drug conjugates
for tumor specific application, which can simultaneously address the
efficacy and safety concerns
DataSheet_1_Downregulation of T-cell cytotoxic marker IL18R1 promotes cancer proliferation and migration and is associated with dismal prognosis and immunity in lung squamous cell carcinoma.docx
Immunotherapy can improve the survival of patients with advanced lung squamous cell carcinoma (LUSC). T cytotoxic cells are one of the main members of the immune microenvironment. Herein, we aimed to identify the roles of T-cell cytotoxic markers interleukin 18 (IL18) receptor 1 (IL18R1) in the LUSC progression using bioinformatics, clinical tissue specimen, and cell experiment. We assessed the association between the IL18R1 expression and immune infiltration and IL18R1-related competing RNA network. The IL18R1 expression was downregulated in the LUSC tissues. The IL18R1 expression downregulation was associated with diagnosis and short overall survival and disease-specific survival, and it was also an independent risk factor for dismal survival time in LUSC. IL18R1-related nomograms predicted the survival time of patients with LUSC. IL18R1 overexpression inhibited the proliferation, migration, and invasion of LUSC cells. The IL18R1 expression was significantly associated with the microenvironment (stromal, immune, and estimate scores), immune cells (such as the T cells, cytotoxic cells, CD8 T cells), and immune cell markers (such as the CD8A, PD-1, and CTLA4) in LUSC. AC091563.1 and RBPMS-AS1 downregulation was positively associated with the IL18R1 expression, negatively associated with the miR-128-3p expression, and associated with short disease-specific survival and progression in LUSC. In conclusion, IL18R1 was significantly downregulated and associated with the prognosis and immune microenvironment. IL18R1 overexpression inhibits the growth and migration of cancer cells in LUSC. Furthermore, AC091563.1 and RBPMS-AS1 might compete with IL18R1 to bind miR-128-3p for participating in LUSC progression. These results showed that IL18R1 is a biomarker for evaluating the prognosis of patients with LUSC.</p